TRYPSINIZATION

Trypsinization Trypsinization is defined as the process of dissociating a cell using trypsin. Trypsin is a proteolytic enzyme which breaks down proteins and digests adherent cells in the vessel in which they are cultured.

Types of Trypsinization Warm Trypsinization Cold Trypsinization The whole content is then placed on ice and soaked in cold trypsin for 4-6 hours to allow the penetration of enzymes in tissue. The tissue is incubated at 36.50C for 20-30 minutes. No stirring or centrifugation is required . Gives higher yield of viable cells with improved survival after 24 h culture. The trypsin is removed by centrifugation after 3-4 hours during which the complete disaggregation of tissues takes place. The tissue pieces are treated with warm trypsin (36.50c). Centrifugation is required. Lesser yield.