Physiologic activation of nuclear factor kappa-B in the endometrium during the menstrual cycle is altered in endometriosis patients Reinaldo González-Ramos,

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Physiologic activation of nuclear factor kappa-B in the endometrium during the menstrual cycle is altered in endometriosis patients Reinaldo González-Ramos, M.D., Ph.D., Jocelyn Rocco, B.Sc., Candy Rojas, B.Sc., Hugo Sovino, M.D., Andrea Poch, B.Sc., Paulina Kohen, B.Sc., Carlos Alvarado-Díaz, B.Sc., Luigi Devoto, M.D. Fertility and Sterility Volume 97, Issue 3, Pages 645-651 (March 2012) DOI: 10.1016/j.fertnstert.2011.12.006 Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Electrophoretic mobility shift assay of nuclear factor (NF) κB. (A) Healthy women endometrial samples. (B) Endometriosis patients endometrial samples. Lanes 1–8 = nuclear extracts of endometrial samples from different patients; C+ = positive control sample. Fertility and Sterility 2012 97, 645-651DOI: (10.1016/j.fertnstert.2011.12.006) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 2 p65-DNA binding immunodetection assay. The absorbance of the p65 protein–oligonucleotide immunodetected complexes was measured relative to positive control sample (C+). Control endometrial samples were obtained from healthy women, and patient endometrial samples were obtained from endometriosis patients. Box plots show the median, 25th and 75th percentiles, and range. ∗P<.05. Fertility and Sterility 2012 97, 645-651DOI: (10.1016/j.fertnstert.2011.12.006) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 3 p65 immunohistochemistry. (A) Menstrual endometrium. (B) Proliferative endometrium. (C) Secretory endometrium. (C−) Negative control sample. (D) p65 expression level score (ELS) on endometrial epithelial cells (EEC) and endometrial stromal cells (ESC) in healthy women endometrium. ∗P<.05. (E) p65 ELS on EEC and ESC in endometriosis patients endometrium. ∗∗P<.05. Fertility and Sterility 2012 97, 645-651DOI: (10.1016/j.fertnstert.2011.12.006) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions