The nature and origin of binucleate cells in human preimplantation embryos: relevance to placental mesenchymal dysplasia L. Xanthopoulou, J.D.A. Delhanty,

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Presentation transcript:

The nature and origin of binucleate cells in human preimplantation embryos: relevance to placental mesenchymal dysplasia L. Xanthopoulou, J.D.A. Delhanty, A. Mania, T. Mamas, P. Serhal, S.B. SenGupta, A. Mantzouratou Reproductive BioMedicine Online Volume 22, Issue 4, Pages 362-370 (April 2011) DOI: 10.1016/j.rbmo.2011.01.001 Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Nuclear abnormalities seen in human preimplantation embryos. Reproductive BioMedicine Online 2011 22, 362-370DOI: (10.1016/j.rbmo.2011.01.001) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Fluorescence in-situ hybridization results from two binucleate blastomeres from two different embryos from the same preimplantation genetic screening patient using probes for chromosomes 13 (green), 18 (aqua) and 21 (orange). (A) Diploid signals with tetrasomy 18. (B) Overall diploid signals with trisomy 18. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Reproductive BioMedicine Online 2011 22, 362-370DOI: (10.1016/j.rbmo.2011.01.001) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Mononucleate biopsied blastomere from a preimplantation genetic screening case found to be haploid for the chromosomes tested after three rounds of fluorescence in-situ hybridization, probed for: (A) chromosomes 13 (green), 18 (aqua) and 21 (orange); (B) chromosomes 15 (orange), 16 (yellow, a cohybridization of the centromeric probe for chromosome 16 in orange and green) and 22 (green); and (C) chromosomes X (green), Y (orange) and 18 (aqua). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Reproductive BioMedicine Online 2011 22, 362-370DOI: (10.1016/j.rbmo.2011.01.001) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

Figure 4 Results from cases of preimplantation genetic diagnosis for monogenic disorders. Figure shows maternal alleles, paternal alleles and alleles present in the biopsied cells. (A) In this case the biopsied cell was haploid containing only the paternal genome. (B) Here the biopsied cell contained a copy of the maternal genome and two copies of the paternal genome. Reproductive BioMedicine Online 2011 22, 362-370DOI: (10.1016/j.rbmo.2011.01.001) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions