Analysis and interpretation of difficult samples—The interaction of a DNA quantification kit with the right STR assay may facilitate processing of critical.

Slides:



Advertisements
Similar presentations
The most efficient STR loci in forensic genetics in population of central Poland R. Jacewicz, M. Jedrzejczyk, J. Berent Forensic Science International:
Advertisements

A comparison of AmpFlSTR Identifiler™ Kit versus AmpFlSTR Identifiler Plus™ Kit in challenging bone samples by using normal and increased PCR cycle number 
DNA persistence in soft tissue comparing vodka and absolute ethanol
A comparison of AmpFlSTR Identifiler™ Kit versus AmpFlSTR Identifiler Plus™ Kit in challenging bone samples by using normal and increased PCR cycle number 
On the effect of shooting distance, ballistic model construction, doping and weapon type on the simultaneous analysis of DNA and RNA from backspatter.
A. Verzeletti, V. Cortellini, S. Cisana, S. Pretto, N. Cerri, F
The visualization and quantification of cell nuclei in telogen hair roots by fluorescence microscopy, as a pre-DNA analysis assessment  Tom Boonen, Kathy.
Considering a performance test of forensic genomics system on massively parallel sequencing technology  P. Sukawutthiya, T. Sathirapatya, K. Vongpaisarnsin 
From sub-source to source: Interpreting results of biological trace investigations using probabilistic models  Wessel T. Oosterman, Bas Kokshoorn, Petra.
S. Severini, M. Lancia, S. Massetti, A. Coletti, L. Carlini, E
N. Cerri, S. Cisana, V. Cortellini, A. Verzeletti, A. Agostino, F
An evaluation of miniSTR markers for casework applications
Performance of the RapidHIT™200
A. Barbaro, P. Cormaci, G. Falcone 
The Investigator® ESSplex Plus Kit: Fast, sensitive, and robust amplification of the European Standard Set of loci  Mario Scherer, Daniel Müller, Sebastian.
Development and validation of the new Investigator® Argus X-12 QS Kit
Investigator® Quantiplex Kit: For reliable quantification of human DNA in forensic samples  Francesca Di Pasquale, Stefan Cornelius, Margaretha König,
Forensic identification of 12 mammals species based on size variation of mitochondrial cytochrome b gene using multiplex PCR assay  M. Črček, K. Drobnič 
Probabilistic modelling for DNA mixture analysis
C. Davies, J. Thomson, F. Kennedy 
The effect of increased cycle numbers using the Quantifiler® Duo DNA Quantification Kit (AB) on the detection of minute amounts of male DNA in mixtures.
Considering a performance test of forensic genomics system on massively parallel sequencing technology  P. Sukawutthiya, T. Sathirapatya, K. Vongpaisarnsin 
DNA typing of trace DNA recovered from different areas of sandals found at a homicide crime scene investigation: A comparative study  Samuel T.G. Ferreira,
Stine Frisk Fredslund, Helle Smidt Mogensen, Niels Morling 
Establishment of Italian national DNA database and the central laboratory: Some aspects  R. Biondo, F. De Stefano  Forensic Science International: Genetics.
Yan Liu, Li Li, Chengtao Li, Zhenmin Zhao 
Quantification of trace amounts of human and non-human mitochondrial DNA (mtDNA) using SYBR Green and real time PCR  Shanan S. Tobe, Adrian Linacre  Forensic.
A custom software solution for forensic mtDNA analysis of MiSeq data
R. Alghafri, S. Alhammadi, K. Amiri 
Population data for SE33 locus in United Arab Emirates Arab population
Development of a SNP-STRs multiplex for forensic identification
Combining DNA evidence for greater match information
Oskar Hansson, Peter Gill 
J. Hedman, L. Albinsson, L. Norén, R. Ansell 
A. Verzeletti, V. Cortellini, S. Cisana, S. Pretto, N. Cerri, F
Genetic structure of Moroccan population using 15 STRs of NGM kit
P. Dario, A. R. Oliveira, T. Ribeiro, M. J. Porto, J. Costa Santos, D
Insertion/deletion polymorphisms: A multiplex assay and forensic applications  Rui Pereira, Christopher Phillips, Cíntia Alves, António Amorim, Ángel Carracedo,
Ronny Decorte, Chi Fung Liu, Nancy Vanderheyden, Jean-Jacques Cassiman 
DIP–STR: A new marker for resolving unbalanced DNA mixtures
A review of low template STR analysis in casework using the DNA SenCE post-PCR purification technique  T. Gross, J. Thomson, S. Kutranov  Forensic Science.
A comparison of AmpFlSTR Identifiler™ Kit versus AmpFlSTR Identifiler Plus™ Kit in challenging bone samples by using normal and increased PCR cycle number 
Improving sample analysis and interpretation using QIAGEN’s latest Investigator® STR multiplex PCR assays with a novel quality sensor  M. Scherer, M.
Introducing a latent variable approach for finding populations in a forensic DNA database  Maarten Kruijver  Forensic Science International: Genetics.
Determination of siblings: A special case report from Halle
C.J. Bruijning-van Dongen, K. Slooten, W. Burgers, W. Wiegerinck 
Direct STR typing from bullet casings
Developmental validation of a fully automated genotyping assay capable of detecting length and sequence variation in the CODIS STR loci  David D. Duncan,
Testing the behavior of GlobalFiler® PCR amplification kit with degraded and/or inhibited biological samples  V. Bogas, M. Carvalho, F. Corte-Real, M.J.
Development of the Investigator STR GO
M. Hara, T. Masuda, A. Takada, T. Miyazaki, H. Suzuki, A. Kido, K
Semen detection: A retrospective overview from 2010
Application of less primer method to commercial kits
Linda Albinsson, Johannes Hedman, Ricky Ansell 
DNA persistence in soft tissue comparing vodka and absolute ethanol
Kpop: A Python package for population genetics analysis
DNA recovery from a 44-year old umbilical cord
Monique Wesselink, Irene Kuiper 
Effects of the most common methods for the enhancement of latent fingerprints on DNA extraction from forensic samples  S. Gino, M. Omedei  Forensic Science.
Development and validation of the Investigator® Quantiplex Pro Kit for qPCR-based examination of the quantity and quality of human DNA in forensic samples 
Li Wang, P.M. Schneider, M.A. Rothschild, P. Bai, W. Liang, Lin Zhang 
Improved performance for forensic casework: Extraction and isolation updates for the Maxwell® 16 instrument  M. Lindner, P.V. Mandrekar, J. Bessetti,
Genetic data for the locus SE33 in a Southern Italy population with AmpFlSTR NGM SElect™ PCR amplification kit  A. Barbaro, P. Cormaci, A. La Marca  Forensic.
Development of a 24-plex Y chromosomal STR loci typing system
Statistical aspects of familial searching
Andreas O. Tillmar, Helena Nilsson, Gunilla Holmlund 
Fast PCR amplification using AmpFlSTR Identifiler: Second report
First application of the Investigator DIPplex indels typing kit for the analysis of ancient DNA samples  C. Hollard, F. Mendisco, C. Keyser, E. Crubézy,
Discrepancies between forensic DNA databases
A.M. Ölçen, G. Filoğlu, H. Altunçul, S. Erdem, Ö. Bülbül 
Presentation transcript:

Analysis and interpretation of difficult samples—The interaction of a DNA quantification kit with the right STR assay may facilitate processing of critical trace material  F. Di Pasquale, S. Cornelius, M. König, M. Scherer, A. Prochnow, R. Peist  Forensic Science International: Genetics Supplement Series  Volume 5, Pages e407-e408 (December 2015) DOI: 10.1016/j.fsigss.2015.09.161 Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Quality Sensor information. (A) 500pg control DNA 9948 was amplified. (B) 15μl template containing 500pg Control DNA 9948 was amplified, resulting in a final hematin concentration of 900μM. Forensic Science International: Genetics Supplement Series 2015 5, e407-e408DOI: (10.1016/j.fsigss.2015.09.161) Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions