Fig. 2 CD32+ cells have a distinctively different phenotype compared to CD32− cells. CD32+ cells have a distinctively different phenotype compared to CD32−

Slides:

Advertisements

Similar presentations

Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy Erik Wambre, PhD, Jonathan.

Advertisements

Pregnancy persistently affects memory T cell populations

High-dimensional analysis of lymphoid CD4+ T cells identified distinct TFH cell subsets in HIV+ patients and HCs. High-dimensional analysis of lymphoid.

Dominant IL-21 expression in TFH cells correlate with B cell pathology in HIV-infected LNs. Dominant IL-21 expression in TFH cells correlate with B cell.

Identification of combination treatment–responsive effector/memory tumor-infiltrating CD8+ T cell population. Identification of combination treatment–responsive.

Fig. 1. TP is highly expressed in myeloma.

Selective expansion of polyfunctional pathogen-specific CD4+ T cells in HIV-1–infected patients with immune reconstitution inflammatory syndrome by Yolanda.

Fig. 4. Primary human metastatic melanomas contain CCL21-expressing LECs, and expression of VEGFC positively correlates with hallmarks of tumor inflammation.

Clonal expansion and TCR-independent differentiation shape the HIV-specific CD8+ effector-memory T-cell repertoire in vivo by Dirk Meyer-Olson, Brenna.

Fig. 5. Correlation between CD34+CD45RA−CD90+ cell dose, engraftment success, and onset of neutrophil/platelet recovery in nonhuman primates. Correlation.

Fig. 4. Liver HBV mRNA paired-end sequencing reads in HBeAg-positive and HBeAg-negative chimpanzees. Liver HBV mRNA paired-end sequencing reads in HBeAg-positive.

Volume 147, Issue 1, Pages e7 (July 2014)

Fig. 1 Increasing UCB cell dose impairs short-term progenitor cell engraftment. Increasing UCB cell dose impairs short-term progenitor cell engraftment.

Fig. 3. Frequencies of amino acids at critical PGT121 and contact sites in the SHIV-SF162P3 challenge stock. Frequencies of amino acids at critical.

Volume 20, Issue 12, Pages (September 2017)

Fig. 6. Increased efficacy of immunotherapy in lymphangiogenic B16 melanomas depends on CCR7 signaling before therapy and local activation and expansion.

Fig. 5 A competent Fc is required for the antitumor immune response.

rSema4A increases the relative number of CD4+CD25+ HuT cells.

Figure 2 Brain-infiltrating immune cells mainly consist of CD8+ memory T cells Immunofluorescence staining of brain-infiltrating immune cells. Brain-infiltrating.

Dot plots of trisomic versus fetal fractions for cohorts 1 and 2

T-cell receptor diversity is selectively skewed in T-cell populations of patients with Wiskott-Aldrich syndrome Junfeng Wu, MD, Dawei Liu, MS, Wenwei.

Fig. 2. Best model fits. Best model fits. Illustration of the best model fits for the (A) basic, (B) continuous, and (C) cluster models. See Table 1 and.

Current model of CD8+ T-cell central and effector memory generation for mouse and human T cells. Current model of CD8+ T-cell central and effector memory.

Fig. 3 In situ vaccination with CpG and anti-OX40 is therapeutic in a spontaneous tumor model. In situ vaccination with CpG and anti-OX40 is therapeutic.

Fig. 4. Actin polymerization rhythms are required for circadian regulation of adhesion and wound-healing efficacy by fibroblasts. Actin polymerization.

Fig. 7 BRD0705 impairs colony formation in AML cell lines and patient cells and shows in vivo efficacy in multiple AML mouse models. BRD0705 impairs colony.

Conformational ensembles of apo and ligated FimH variants in solution

TCF1 expression marks self-renewing human CD8+ T cells

Fig. 4. Analysis of T cell responses according to treatment and in peptide-treated C-peptide responders and nonresponders. Analysis of T cell responses.

Immunological phenotypes of CD4+ T cells preferentially infected by HIV. PBMCs exposed to HIV-1BaL (MOI 0.01) were cultured for 10 d. Immunological phenotypes.

Fig. 5 Local gel scaffold for T cell memory response.

Fig. 1 HIV transcription profiles in freshly isolated CD4+ T cells and PBMCs from HIV-infected patients on ART suggest blocks to HIV transcriptional elongation,

Volume 38, Issue 1, Pages (January 2013)

Kevin G. Haworth, Christina Ironside, Zachary K. Norgaard, Willimark M

Fig. 1 Effect of preinfection β7Hi CD45RA−CD4+ T cell frequency on HIV acquisition risk in CAPRISA 004 study. Effect of preinfection β7Hi CD45RA−CD4+ T.

FIP200 loss links to poor autophagy and high apoptosis in naïve T cells in tumor. FIP200 loss links to poor autophagy and high apoptosis in naïve T cells.

Fig. 3. VEGFR-3 signaling increases infiltration of naïve T cells in a CCR7-dependent manner. VEGFR-3 signaling increases infiltration of naïve T cells.

Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy Erik Wambre, PhD, Jonathan.

Fig. 2. Efficient HDR in primary T cells leading to introduction of a GFP expression cassette within the CCR5 locus. Efficient HDR in primary T cells leading.

Fig. 2 Activation of freshly isolated CD4+ T cells from HIV-infected patients on ART results in successive increases in elongated, polyadenylated, and.

Fig. 7 Vaccine-induced influenza-specific B cells are not maintained in peripheral blood. Vaccine-induced influenza-specific B cells are not maintained.

Vaccination induces activation of cTFH cells and transient ASCs

IIV induces CD21hiCD27+ and CD21loCD27+ influenza-specific B cells

Fig. 3 Activation of freshly isolated CD4+ T cells from HIV-infected patients on ART selectively reverses baseline blocks to splicing, elongation, and.

Fig. 5 Extended local release of R848 increases the number of innate and adaptive antitumor immune cells and cytokines. Extended local release of R848.

Statistical evaluation of CD56+ NK cell subset data.

Correlation of reovirus RNA/protein with proliferating tumor cells

VH usage of cross-reactive B cells induced by H5N1 or H7N9 vaccination

CD4-TEMRA cells show marked clonal expansion.

Donor and recipient BAL T cells are phenotypically and functionally memory T cells. Donor and recipient BAL T cells are phenotypically and functionally.

Differential expression of TRM markers by donor- and recipient-derived T cells with time. Differential expression of TRM markers by donor- and recipient-derived.

Blood Tfr cells are lymphoid tissue–derived Tfr precursors.

Fig. 4. Peanut-specific TH2A cells are specifically targeted during immunotherapy. Peanut-specific TH2A cells are specifically targeted during immunotherapy.

MP cells are generated from naïve cells in the periphery.

CD8+ T-cell subsets. CD8+ T-cell subsets. There are a number of different CD8+ T-cell subsets. Naïve, T stem cell (TSCM) and T central memory (TCM) cells.

CD4-CTL precursor cells in the TEMRA subset.

CD4-TEMRA cells are heterogeneous across donors.

MP cells, but not pathogen-elicited effector CD4+ T lymphocytes, rapidly produce IFN-γ during T. gondii infection independently of pathogen antigens. MP.

CD4+CLA+CD103+ T cells constitute a unique cell population in human blood. CD4+CLA+CD103+ T cells constitute a unique cell population in human blood. (A)

T-CTLs are CD8+ TEMRA cells that can be expanded by IL-15 and IL-2 through selective proliferation and can be induced by LCs. T-CTLs are CD8+ TEMRA cells.

Shared phenotype of CD4+CLA+CD103+ T cells from human blood and skin.

MR1Ts recognized by the hpMR1+EC tetramer are more likely to be TRAV1-2−. MR1Ts recognized by the hpMR1+EC tetramer are more likely to be TRAV1-2−. PBMCs.

by Zaza M. Ndhlovu, Samuel W

A phenotypically and functionally distinct human TH2 cell subpopulation is associated with allergic disorders by Erik Wambre, Veronique Bajzik, Jonathan.

CD4-CTL effectors share TCR clonotypes with CD4-CTL precursors.

Blood Tfr cells are immature but are not committed in the thymus.

Molecular Therapy - Nucleic Acids

IL-9–expressing TH cells are highly enriched in CCR4+/CCR8+ effector memory TH cells. IL-9–expressing THcells are highly enriched in CCR4+/CCR8+effector.

Comparison of MLTC responses to tumor/peptide stimuli at day 18 and day 34 of growth. Comparison of MLTC responses to tumor/peptide stimuli at day 18 and.

Correlation of TAN phenotypes and TIL effector function.

Presentation transcript:

Fig. 2 CD32+ cells have a distinctively different phenotype compared to CD32− cells. CD32+ cells have a distinctively different phenotype compared to CD32− cells. (A) Distribution of CD32+ HLA-DR− within cell subsets in viremic, ART-treated HIV+ and HIV− donors. In the Barcelona cohort, subsets were defined as naïve (CD45RO− CD27+), TTD (CD45RO− CD27−), effector memory T cells (TEM) (CD45RO+ CD27−), and central memory T cells (TCM)/transitional memory T cells (TTM) (CD45RO+ CD27+). In the Philadelphia cohort, subsets were defined as naïve (CD45RA+ CD27+ CCR7+ CD95−), stem memory T cells (TSCM) (CD45RA+ CD27+ CCR7+ CD95+), TTD (CD45RA+ CD27−), TEM (CD45RA− CD27−), TCM (CD45RA− CD27+ CCR7+), and TTM (CD45RA− CD27+ CCR7−). (B) Frequencies of TH subsets within memory CD32+ CD4+ T cells (top) and their distribution within HLA-DR+/− TH2 CD32+ CD4+ T cells (bottom). Not subset refers to those cells that did not fall into the defined TH subsets. (C and D) Frequency of CD38+ (C) and Ki67+ (D) cells in CD32/HLA-DR–expressing memory CD4+ T cells. Representative examples are shown for each marker with overlaid plots showing CD32+ cells (red dots in plots and solid red lines in histograms) over total memory cells (black dots in plots and dotted lines in histograms). (E) Heat map showing the frequency of all measured phenotypic markers in CD32+ and CD32− cells. CD32p, memory CD32+ CD4+ T cells; CD32n, memory CD32− CD4+ T cells. (F) PCA showing the distribution of CD32+ and CD32− cell subsets. In all graphs, a line indicates the median of the group. All data were analyzed using a Friedman test (paired, nonparametric) and corrected for multiple comparisons with Dunnett’s posttest. n = 6 for HIV− controls, n = 12 for HIV+ ART+ individuals, and n = 8 for HIV+ viremic individuals. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. Mohamed Abdel-Mohsen et al., Sci Transl Med 2018;10:eaar6759 Published by AAAS