Hairpin & reporter systems.

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Hairpin & reporter systems. Hairpin & reporter systems. (A) Positions and sense/antisense orientation of dsRNA and reporter sequences relative to mRNAs. Stem loop structures indicate situations at stem termini. (B) Reporter system performance using various “neutral” co-expressed proteins. 3T3 cells were transfected with MosIR and luciferase reporters and one of EGFP-, RFP-, and LacZ-expressing plasmids. Luciferase activities were analyzed 48 h after transfection. Firefly and Renilla graphs show raw luminescence values from one transfection experiment performed in triplicates, the right graph depicts data from four independent experiments performed in triplicates. Error bars = SD. Tomas Demeter et al. LSA 2019;2:e201800289 © 2019 Demeter et al.