Figure 2 P2Y12 expression is upregulated in M2-polarized human microglia(A, B) Using TaqMan quantitative real-time PCR, P2Y12 expression was measured in.

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Figure 2 P2Y12 expression is upregulated in M2-polarized human microglia(A, B) Using TaqMan quantitative real-time PCR, P2Y12 expression was measured in M1- and M2-polarized human microglia (both adult and fetal). P2Y12 expression is upregulated in M2-polarized human microglia(A, B) Using TaqMan quantitative real-time PCR, P2Y12 expression was measured in M1- and M2-polarized human microglia (both adult and fetal). Compared to unpolarized (Unpol.) and M1-polarized cells, M2 microglia had significantly elevated P2Y12 expression. Error bars represent mean ± SEM; n = 5/condition for microglia, n = 3/condition for macrophages; *p < 0.05, ***p < 0.001 compared to unpolarized. (C) Using flow cytometry, gating on the live human microglia population, mean fluorescence intensity of P2Y12 was increased in M2-polarized (red line) adult microglia (D) and fetal microglia (E) compared to resting conditions (blue line). Shaded histogram represents isotype control. Craig S. Moore et al. Neurol Neuroimmunol Neuroinflamm 2015;2:e80 © 2015 American Academy of Neurology