Curcumin Selectively Induces Apoptosis in Cutaneous T-Cell Lymphoma Cell Lines and Patients’ PBMCs: Potential Role for STAT-3 and NF-κB Signaling Chunlei.

Slides:

Advertisements

Similar presentations

IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,

Advertisements

MicroRNA-21 Expression in CD4+ T Cells Is Regulated by STAT3 and Is Pathologically Involved in Sézary Syndrome Leslie van der Fits, Marloes S. van Kester,

Neurotrophins and Their Receptors Stimulate Melanoma Cell Proliferation and Migration Francesca Truzzi, Alessandra Marconi, Roberta Lotti, Katiuscia.

Curcumin (diferuloylmethane) down-regulates the constitutive activation of nuclear factor–κB and IκBα kinase in human multiple myeloma cells, leading to.

NKp46-Specific Expression on Skin-Resident CD4+ Lymphocytes in Mycosis Fungoides and Sézary Syndrome Pierre Schneider, Louis-François Plassa, Philippe.

Aurora Kinase A Is Upregulated in Cutaneous T-Cell Lymphoma and Represents a Potential Therapeutic Target Daniel Humme, Ahmed Haider, Markus Möbs, Hiroshi.

Yongping Shao, Kaitlyn Le, Hanyin Cheng, Andrew E. Aplin

Droxinostat, a Histone Deacetylase Inhibitor, Induces Apoptosis in Hepatocellular Carcinoma Cell Lines via Activation of the Mitochondrial Pathway and.

Impaired Activation of the Nrf2-ARE Signaling Pathway Undermines H2O2-Induced Oxidative Stress Response: A Possible Mechanism for Melanocyte Degeneration.

Constitutive and tumor necrosis factor-α-induced activation of nuclear factor-κB in adenomyosis and its inhibition by andrographolide Bin Li, M.S., Ming.

Role of hypoxia-inducible factor-1 alpha in the regulation of plasminogen activator activity in rat knee joint chondrocytes G. Zhu, Y. Tang, X. Liang,

Interferon-γ Protects from Staphylococcal Alpha Toxin-Induced Keratinocyte Death through Apolipoprotein L1 Anne M. Brauweiler, Elena Goleva, Donald Y.M.

Thorbjørn Krejsgaard, Ulrik Ralfkiaer, Erik Clasen-Linde, Karsten W

KIR3DL2/CpG ODN Interaction Mediates Sézary Syndrome Malignant T Cell Apoptosis Bouchra Ghazi, Nicolas Thonnart, Martine Bagot, Armand Bensussan, Anne.

Insulin-Like Growth Factor-I Enhances Transforming Growth Factor-β-Induced Extracellular Matrix Protein Production Through the P38/Activating Transcription.

Cytokine Effects Induced by the Human Autoallergen α-NAC

Human Keratinocytes Express Functional CD14 and Toll-Like Receptor 4

IFNλ Stimulates MxA Production in Human Dermal Fibroblasts via a MAPK-Dependent STAT1-Independent Mechanism Adewonuola A. Alase, Yasser M. El-Sherbiny,

Resistance to Activation-Induced Cell Death and Bystander Cytotoxicity Via the Fas/Fas Ligand Pathway Are Implicated in the Pathogenesis of Cutaneous.

Wnt5a/β-Catenin Signaling Drives Calcium-Induced Differentiation of Human Primary Keratinocytes Tanja Popp, Dirk Steinritz, Andreas Breit, Janina Deppe,

Overexpression of mutated IκBα inhibits vascular smooth muscle cell proliferation and intimal hyperplasia formation Brian S Zuckerbraun, MD, Carol A.

Avicin D Selectively Induces Apoptosis and Downregulates p-STAT-3, bcl-2, and Survivin in Cutaneous T-Cell Lymphoma Cells Chunlei Zhang, Baoqiang Li,

Valentina Manfé, Edyta Biskup, Peter Johansen, Maria R

Meghan Bliss-Moreau, Cristian Coarfa, Preethi H

Arsenite-Mediated Promotion of Anchorage-Independent Growth of HaCaT Cells through Placental Growth Factor Ichiro Yajima, Mayuko Y. Kumasaka, Shoko Ohnuma,

Histamine Contributes to Tissue Remodeling via Periostin Expression

Akio Horiguchi, Mototsugu Oya, Ken Marumo, Masaru Murai

Interleukin-22 Is Frequently Expressed in Small- and Large-Cell Lung Cancer and Promotes Growth in Chemotherapy-Resistant Cancer Cells Sebastian Kobold,

NF-κB Inhibition through Proteasome Inhibition or IKKβ Blockade Increases the Susceptibility of Melanoma Cells to Cytostatic Treatment through Distinct.

Inhibition of PI3K-AKT-mTOR Signaling Sensitizes Melanoma Cells to Cisplatin and Temozolomide Tobias Sinnberg, Konstantinos Lasithiotakis, Heike Niessner,

The Selective Protein Kinase C β Inhibitor Enzastaurin Induces Apoptosis in Cutaneous T-Cell Lymphoma Cell Lines through the AKT Pathway Christiane Querfeld,

Bernd Rebholz, Kai Kehe, Thomas Ruzicka, Rudolf A. Rupec

Poly(I:C)-Treated Human Langerhans Cells Promote the Differentiation of CD4+ T Cells Producing IFN-γ and IL-10 Laetitia Furio, Hermine Billard, Jenny.

Leslie van der Fits, Jacoba J. Out-Luiting, Cornelis P

SiRNA Knockdown of Ribonucleotide Reductase Inhibits Melanoma Cell Line Proliferation Alone or Synergistically with Temozolomide Jonathan E. Zuckerman,

Transcriptional Regulation of ATP2C1 Gene by Sp1 and YY1 and Reduced Function of its Promoter in Hailey–Hailey Disease Keratinocytes Hiroshi Kawada,

Apoptosis Induction by SAHA in Cutaneous T-Cell Lymphoma Cells Is Related to Downregulation of c-FLIP and Enhanced TRAIL Signaling Nadya Al-Yacoub, Lothar.

Histamine Enhances the Production of Granulocyte-Macrophage Colony-Stimulating Factor via Protein Kinase Cα and Extracellular Signal-Regulated Kinase.

Cyclooxygenase-2 Inhibitor Enhances Whereas Prostaglandin E2Inhibits the Production of Interferon-Induced Protein of 10 kDa in Epidermoid Carcinoma A431

All-Trans-Retinoic Acid Induces Interleukin-8 via the Nuclear Factor-κB and p38 Mitogen-Activated Protein Kinase Pathways in Normal Human Keratinocytes

P38 Mitogen-Activated Protein Kinase Mediates Dual Role of Ultraviolet B Radiation in Induction of Maturation and Apoptosis of Monocyte-Derived Dendritic.

Kellie J. White, Vincent J. Maffei, Marvin Newton-West, Robert A

MiR-223 Regulates Cell Growth and Targets Proto-Oncogenes in Mycosis Fungoides/Cutaneous T-Cell Lymphoma Laura Y. McGirt, Clare M. Adams, Devin A. Baerenwald,

Chi-Hyun Park, Youngji Moon, Chung Min Shin, Jin Ho Chung

TNF-α and IFN-γ Are Potential Inducers of Fas-Mediated Keratinocyte Apoptosis through Activation of Inducible Nitric Oxide Synthase in Toxic Epidermal.

The IL-6 Trans-Signaling-STAT3 Pathway Mediates ECM and Cellular Proliferation in Fibroblasts from Hypertrophic Scar Sutapa Ray, Xiaoxi Ju, Hong Sun,

PPARδ Is a Type 1 IFN Target Gene and Inhibits Apoptosis in T Cells

Mark A. Rovedo, Nancy L. Krett, Steven T. Rosen

Gang Li, Jason A. Bush, Vincent C. Ho

Green Tea Polyphenol Epigallocatechin-3-Gallate Suppresses Collagen Production and Proliferation in Keloid Fibroblasts via Inhibition of the STAT3-Signaling.

IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,

Volume 19, Issue 10, Pages (October 2011)

IFN-α Enhances IL-22 Receptor Expression in Keratinocytes: A Possible Role in the Development of Psoriasis Mikiko Tohyama, Lujun Yang, Yasushi Hanakawa,

Bcl-2 Reduced and Fas Activated by the Inhibition of Stem Cell Factor/KIT Signaling in Murine Melanocyte Precursors Satoko Kimura, Tamihiro Kawakami,

Collagen Synthesis Is Suppressed in Dermal Fibroblasts by the Human Antimicrobial Peptide LL-37 Hyun Jeong Park, Dae Ho Cho, Hee Jung Kim, Jun Young.

Selective Induction of Apoptosis by Histone Deacetylase Inhibitor SAHA in Cutaneous T- Cell Lymphoma Cells: Relevance to Mechanism of Therapeutic Action

Society for Investigative Dermatology 2010 Meeting Minutes

Amy K. Gira, Andrew P. Kowalczyk, Yue Feng, Robert A. Swerlick

Dimethylfumarate Specifically Inhibits the Mitogen and Stress-Activated Kinases 1 and 2 (MSK1/2): Possible Role for its Anti-Psoriatic Effect Borbala.

IL-17A Upregulates Keratin 17 Expression in Keratinocytes through STAT1- and STAT3- Dependent Mechanisms Xiaowei Shi, Liang Jin, Erle Dang, Ting Chang,

1α,25-Dihydroxyvitamin D3 Stimulates Activator Protein 1 DNA-Binding Activity by a Phosphatidylinositol 3-Kinase/Ras/MEK/Extracellular Signal Regulated.

STAT5a/PPARγ Pathway Regulates Involucrin Expression in Keratinocyte Differentiation Xiuju Dai, Koji Sayama, Yuji Shirakata, Yasushi Hanakawa, Kenshi.

Myeloid Differentiation Factor 88 Regulates Basal and UV-Induced Expressions of IL-6 and MMP-1 in Human Epidermal Keratinocytes Youngae Lee, Hyunjung.

Nan-Lin Wu, Te-An Lee, Te-Lung Tsai, Wan-Wan Lin

Protein Kinase C-Dependent Upregulation of miR-203 Induces the Differentiation of Human Keratinocytes Enikö Sonkoly, Tianling Wei, Elizabeth Pavez Loriè,

Galectin-3 Protects Keratinocytes from UVB-Induced Apoptosis by Enhancing AKT Activation and Suppressing ERK Activation Jun Saegusa, Daniel K. Hsu, Wei.

Low FAS/CD95 Expression by CTCL Correlates with Reduced Sensitivity to Apoptosis that Can Be Restored by FAS Upregulation Jianqiang Wu, Minakshi Nihal,

Deon G. Uffort, Elizabeth A. Grimm, Julie A. Ellerhorst

Bcl-2 and bcl-xL Antisense Oligonucleotides Induce Apoptosis in Melanoma Cells of Different Clinical Stages Robert A. Olie, Christoph Hafner, Renzo Küttel,

Roland Houben, Sonja Ortmann, Astrid Drasche, Jakob Troppmair, Marco J

Presentation transcript:

Curcumin Selectively Induces Apoptosis in Cutaneous T-Cell Lymphoma Cell Lines and Patients’ PBMCs: Potential Role for STAT-3 and NF-κB Signaling Chunlei Zhang, Baoqiang Li, Xiang Zhang, Parul Hazarika, Bharat B. Aggarwal, Madeleine Duvic Journal of Investigative Dermatology Volume 130, Issue 8, Pages 2110-2119 (August 2010) DOI: 10.1038/jid.2010.86 Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Effect of curcumin on cell viability in CTCL cell lines. Cells (5 × 104) were aliquoted into a 96-well culture plate and grown in medium with or without 5, 10, or 20μM curcumin for 24 or 48hours. Cell viability was determined by comparing untreated controls with treated groups using the CellTiter 96 AQueous One Solution Cell Proliferation Assay performed in triplicate. Each point represents mean±SD of triplicate determinations. *Significantly different from control values (n=3; P<0.05). CTCL, cutaneous T-cell lymphoma. Journal of Investigative Dermatology 2010 130, 2110-2119DOI: (10.1038/jid.2010.86) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Effect of curcumin on apoptosis induction in CTCL cell lines. Three cell lines were treated with or without 5, 10, or 20μM curcumin for 24 or 48hours. Annexin V binding was carried out using the Annexin V-FITC Detection Kit. (a) Curcumin induced a concentration- and time-dependent apoptosis in the HH cell line. (b) Each point represents the percentage (mean±SD of triplicate determinations) of both annexin V+PI− and annexin V+PI+ cells. *Significantly different from control values (n=3; P<0.05). CTCL, cutaneous T-cell lymphoma; FITC, fluorescein isothiocyanate; PI, propidium iodide. Journal of Investigative Dermatology 2010 130, 2110-2119DOI: (10.1038/jid.2010.86) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Effect of curcumin on the expression of STAT-3/p-STAT-3 proteins and STAT-3 mRNA in CTCL cell lines and in patients’ PBMCs. CTCL cells were treated with or without 10μM curcumin for 24hours. (a) Expression of STAT-3 and p-STAT-3 proteins was evaluated by western blots. The protein levels were quantified and expression levels of STAT-3 protein from untreated control cells were used as 100%. (b) Expression of STAT-3 mRNA was examined by quantitative real-time PCR assays. The expression levels of normalized STAT-3 mRNA from untreated control cells were used as 100%. The results were expressed as mean±SD in three independent experiments. *Significantly different from control values (n=3; P<0.05). CTCL, cutaneous T-cell lymphoma; PBMC, peripheral blood mononuclear cell; STAT, signal transducer and activator of transcription. Journal of Investigative Dermatology 2010 130, 2110-2119DOI: (10.1038/jid.2010.86) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Effect of curcumin on the expression of apoptosis-related proteins and mRNAs in CTCL cell lines and in patients’ PBMCs. CTCL cells were treated with or without 10μM curcumin for 24hours. (a) Expression of apoptosis-related proteins was evaluated by western blots. The protein levels were quantified and expression levels of bcl-2 and survivin proteins from untreated control cells were used as 100%. (b) Expression of bcl-2 and survivin mRNAs was examined by quantitative real-time PCR assay. The expression levels of normalized bcl-2 and survivin mRNAs from untreated control cells were used as 100%. The results were expressed as mean±SD in three independent experiments. *Significantly different from control values (n=3; P<0.05). CTCL, cutaneous T-cell lymphoma; PBMC, peripheral blood mononuclear cell. Journal of Investigative Dermatology 2010 130, 2110-2119DOI: (10.1038/jid.2010.86) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Curcumin inhibited DNA binding of NF-κB and IκB-α phosphorylation in CTCL cell lines and in patients’ PBMCs. CTCL cells were treated with or without 10μM curcumin for 24hours. (a) DNA-binding activity of NF-κB was determined by EMSA using biotin-labeled NF-κB probe. (b) Expression of p-IκB-α and IκB-α proteins was evaluated by western blots. The protein levels were quantified and the ratio of p-IκB-α and IκB-α protein expression from untreated control cells was used as 100%. CTCL, cutaneous T-cell lymphoma; EMSA, electrophoretic mobility-shift assay; NF-κB, nuclear factor-κB; PBMC, peripheral blood mononuclear cell. Journal of Investigative Dermatology 2010 130, 2110-2119DOI: (10.1038/jid.2010.86) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 The autophagy inhibitor protected curcumin-induced cell growth inhibition in CTCL cell lines. Three CTCL cell lines were pretreated with or without 10μM chloroquine (CQ) for 24hours, followed by 10μM curcumin for 48hours. Cell viability was measured using the CellTiter 96 AQueous One Solution Cell Proliferation Assay performed in triplicate. Each point represented mean±SD of triplicate determinations. CTCL, cutaneous T-cell lymphoma. Journal of Investigative Dermatology 2010 130, 2110-2119DOI: (10.1038/jid.2010.86) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions