PRG‐1R346T: loss‐of‐function mutation reduced LPA internalization due to altered O‐glycosylation PRG‐1R346T: loss‐of‐function mutation reduced LPA internalization.

Slides:

Advertisements

Similar presentations

Expression of follicular cell‐derived VEGF‐A and endothelial VEGFR is altered by different circulating TSH level Expression of follicular cell‐derived.

Advertisements

Effects of inhibiting inflammatory cell recruitment on fracture healing Treatment with CCR2 antagonist, INCB3344, led to impaired fracture healing compared.

Role of TNF in fracture healing in vivo Addition of rhTNF at the fracture site on days 0 and 1 led to augmented healing, indicated by increased % callus.

Overexpression of PAI‐1 reverses the arsenite‐mediated effect on senescence Overexpression of PAI‐1 reverses the arsenite‐mediated effect on senescence.

Morphology of white adipose tissue and muscle in S1/3 KO mice of different ages Morphology of white adipose tissue and muscle in S1/3 KO mice of different.

Loss of Hdac3 impairs Ar signaling in mouse prostate tissues and has no effect on apoptosis Loss of Hdac3 impairs Ar signaling in mouse prostate tissues.

Phosphorylation of RelA Ser311 in vivo.

Loss of USP8 or HIF1α impairs endocytic recycling required for ciliogenesis Kinetic analysis of transferrin recycling measured by flow cytometry. Loss.

GLUT3 is a direct target of TCF4/β‐catenin

The HDAC3 inhibitor suppresses SPOP‐mutated prostate cancer cell growth The HDAC3 inhibitor suppresses SPOP‐mutated prostate cancer cell growth A22Rv1.

Phosphorylation of EL1 on SLR1 is crucial for GA signalling.

PbA‐specific CD4 T‐cell responses are maximal at day 6 post‐pRBC infection PbA‐specific CD4 T‐cell responses are maximal at day 6 post‐pRBC infection Proportion.

Control of IL‐6 synthesis by ζPKC and RelA Ser311 phosphorylation.

[68Ga]Pentixafor PET imaging of MM xenografts Real‐time PCR analysis of cxcr4 transcript expression levels in HeLa (negative control) and in MM cell lines.

Differential accumulation of substrates in vivo

Disease‐causing mutations influence the association of CSPα with partner proteins Disease‐causing mutations influence the association of CSPα with partner.

ECM29 mediates palbociclib‐induced proteasomal activation and may function as a putative biomarker for palbociclib treatment efficacy in breast cancer.

HPV‐E7 enhances chemotherapy‐mediated cellular stress and CerS1/ceramide‐mediated lethal mitophagy HPV‐E7 enhances chemotherapy‐mediated cellular stress.

TGF‐β1 and Sema3A are downregulated in vivo by increasing VEGF doses Muscles were harvested 7 days after implantation of V Low, V Med, and V High clones.

EphB4 regulates VEGF‐induced phosphorylation of endothelial ERK1/2 downstream of VEGF‐R2 activation EphB4 regulates VEGF‐induced phosphorylation of endothelial.

Enhancement of basal mitophagy by USP30 depletion is dependent on PINK1 Enhancement of basal mitophagy by USP30 depletion is dependent on PINK1 Representative.

Impaired fear extinction precedes memory decline in Fmn2 mutant mice

MiR‐199a directly targets ERBB2 and ERBB3, whereas miR‐125b targets ERBB3. miR‐199a directly targets ERBB2 and ERBB3, whereas miR‐125b targets ERBB3. (A)

Stat3 promotes lung fibrosis and collagen synthesis independent of Smad3.A.Masson's trichrome stained sections of lung from gp130wt (wt), Smad3−/− (Smad3−/−),

HPV‐E7/RB axis regulates ceramide‐dependent mitophagy via E2F5 activation HPV‐E7/RB axis regulates ceramide‐dependent mitophagy via E2F5 activation AUM‐SCC‐47.

KLK7 is involved in the Aβ degradation activity

Integrated protein co‐expression and differential expression across the ALS‐FTD disease continuum Integrated protein co‐expression and differential expression.

PEX13 is required for Sindbis virophagy but not general autophagy

Characterization of oligo#5 and its derivatives

Mitochondrial ceramide is involved in HPV‐E7‐mediated lethal mitophagy

FFAT motif‐dependent recruitment of MOSPD2 in ER–endosome contacts by STARD3NL FFAT motif‐dependent recruitment of MOSPD2 in ER–endosome contacts by STARD3NL.

Nec‐1 inhibits the phosphorylation and aggregation of tau

Hdac3 deletion decreases AKT phosphorylation and tumor growth in Pten knockout prostate cancer Hdac3 deletion decreases AKT phosphorylation and tumor growth.

HMGB1 reverses mitochondrial DNA damage caused by mutant Atxn1 The mitochondrial DNA amplification assay with cerebellar tissue revealed that mitochondrial.

MOSPD2 silencing affects interorganelle contact sites organization

VAP protein knockdown abolishes STARD3‐mediated cholesterol accumulation in endosomes VAP protein knockdown abolishes STARD3‐mediated cholesterol accumulation.

ER stress response and susceptibility to apoptosis are regulated by TFEB and TFE3 ER stress response and susceptibility to apoptosis are regulated by TFEB.

IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice ARepresentative.

Validation of ADORA2A and GPR37 interaction in HEK‐293 cells and native tissue Validation of ADORA2A and GPR37 interaction in HEK‐293 cells and native.

Impact of ONX 0914 on abundance and dissemination of monocytes/macrophages during CVB3 infection Impact of ONX 0914 on abundance and dissemination of monocytes/macrophages.

Effects of ERK–GFP expression levels and cell density on ERK phosphorylation and oscillations. Effects of ERK–GFP expression levels and cell density on.

DOK7 gene therapy suppresses motor nerve terminal degeneration at the NMJ in ALS mice DOK7 gene therapy suppresses motor nerve terminal degeneration at.

HPV‐E7 targets RB for induction of ceramide‐dependent mitophagy

miR‐34a‐5p acts in PDGFRα+ cells to block lung alveolarization

Assessment of proliferation status of PDGFRα+ cells in developing mouse lungs Assessment of proliferation status of PDGFRα+ cells in developing mouse lungs.

R1441G‐LRRK2 co‐localizes with Rab10 in the periphery and Rab8 near the nucleus R1441G‐LRRK2 co‐localizes with Rab10 in the periphery and Rab8 near the.

STARD3‐mediated cholesterol accumulation in endosomes does not alter cholesterol homeostasis STARD3‐mediated cholesterol accumulation in endosomes does.

Drp1 knockdown prevents mitophagy

VAP protein knockdown abolishes STARD3‐mediated cholesterol accumulation in endosomes VAP protein knockdown abolishes STARD3‐mediated cholesterol accumulation.

Therapeutic role of TR in HFD‐established diabetic mice

Both E102Q and L95fs Sig1R variants are unstable and lose their abilities to control Ca2+ flux in Neuro2a cells Both E102Q and L95fs Sig1R variants are.

ADAM8 knockdown profoundly decreases orthotopic tumor formation, CTC spread and metastasis A–DStable ADAM8 KD (shA8) clones were characterized in vitro.

The CHCH domain is necessary for mitochondrial import of CHCHD10

Ubqln1 interacts with Ubqln4.

Mass spectrometric validation of DLG5 as a CDKL5 substrate

LXRα enhances glucose uptake and O‐GlcNAc signaling via activation of the hexosamine biosynthetic pathway (HBP) in cultured cardiomyocytes LXRα enhances.

Activation of Drp1 by E2F5‐Drp1 complex enhances HPV‐E7/ceramide‐mediated lethal mitophagy Activation of Drp1 by E2F5‐Drp1 complex enhances HPV‐E7/ceramide‐mediated.

UCN‐01 inhibits FoxO3 inactivation in human lung fibroblasts stimulated with growth factors UCN‐01 inhibits FoxO3 inactivation in human lung fibroblasts.

Therapeutic activity of gal‐encapsulated cytotoxic drugs on tumor xenografts Therapeutic activity of gal‐encapsulated cytotoxic drugs on tumor xenografts.

PKAN human neurons show altered oxidative status

Scribble PDZ1 preferentially interacts with p‐1 and p‐3 phosphorylated ligands as shown by affinity determinations (MST and ITC), NMR structure, mutational.

Impact of ONX 0914‐induced neutrophilia during CVB3 infection

Effect of D‐Asp treatment on myelination and remyelination in cerebellar organotypic slices Effect of D‐Asp treatment on myelination and remyelination.

Symptomatic rescue of double‐transgenic mice without decreased protein aggregation The expression of exogenous HMGB1‐FLAG (detected with an anti‐FLAG antibody)

Single‐molecule dynamics of the active mutant, hDIA1(R1204X), in XTC cells Single‐molecule dynamics of the active mutant, hDIA1(R1204X), in XTC cells A–DGFP‐tagged.

ADAM8 is induced by hypoxia and promotes angiogenesis ASixteen h after plating, cells were cultured under normoxic (−) or hypoxic (+, 1% O2) conditions.

Ankyrin domain residues permit activation of LRRK2 by Rab29

Absence of miR‐21 in macrophages promotes foam cell formation

The GBS hemolytic pigment/lipid toxin induces caspase 1 activation and pyroptosis THP‐1 macrophages were treated with GBS pigment, and caspase 1 activation.

Western blotting for GluR1.

Presentation transcript:

PRG‐1R346T: loss‐of‐function mutation reduced LPA internalization due to altered O‐glycosylation PRG‐1R346T: loss‐of‐function mutation reduced LPA internalization due to altered O‐glycosylation APRG‐1 and PRG‐1R346T are expressed on HEK293 cell membranes.BConventional fluorescence images of transfected and TopFluor (TF)‐LPA‐stimulated cells after washing with 0.001% SDS. Scale bar: 10 μm.C, DFACS analysis of internalized TF‐LPA of PRG‐1‐ and PRG‐1R346T‐transfected cells (n = 7 each, **P = 0.0015; t‐test).E, FIncubation with C‐17 LPA and subsequent washing as described above, led to a significant increase of TF‐LPA in PRG‐1‐expressing cells but not in PRG‐1R346T‐expressing cells. In line, C‐17 MG, a degradation product of C‐17 LPA, was significantly increased in PRG‐1‐expressing cells but not in control or PRG‐1R346T‐expressing cells (n = 4 each; Kruskal–Wallis test (for C‐17 LPA P = 0.0066; for C‐17 MAG P = 0.0048) with Dunn's multiple comparison test, *P < 0.05 for C‐17 LPA and C‐17 MAG).GQuantitative assessment of S347 phosphorylation using isotopically labeled peptides and mass spectrometry of purified wild‐type PRG‐1 and PRG‐1R346T from stable transfected HEK293 cells revealed low total phosphorylation levels for both protein variants (n = 3 measurements).HTF‐LPA uptake as analyzed by FACS was significantly decreased in S347A and S347D mutant PRG‐1 (n = 12 per condition, ***P < 0.0001; one‐way ANOVA with Bonferroni's multiple comparison test).IWestern blot (WB) of IP using a specific PRG‐1 antibody revealed decreased levels of O‐GlcNAc in PRG‐1R346T when compared to wild‐type PRG‐1.JDensitometric analysis of n = 7 WB from IP of PRG‐1‐ and PRG‐1R346T‐expressing cells revealed a significantly decreased O‐GlcNAc amount in PRG‐1R346T (unpaired t‐test, *P = 0.0107).Data information: All experiments were done in parallel. Bar diagrams represent mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. Johannes Vogt et al. EMBO Mol Med. 2016;8:25-38 © as stated in the article, figure or figure legend