(A) Observed significant protein fold‐changes during the fed–fasting transition in C57BL/6J (B6) and 129Sv (S9) mice fed with a normal diet (T0). (A) Observed significant protein fold‐changes during the fed–fasting transition in C57BL/6J (B6) and 129Sv (S9) mice fed with a normal diet (T0). Significance threshold was set at adjusted P‐value of 0.05. (B) Scatter plot of P‐values associated with the observed changes when comparing both mouse strains fed in normal diet—i.e., 0 weeks (T0, x axis); and after 12 weeks of high‐fat diet (T2, y axis). The plot is divided into three main parts: (i) proteins with a different fasting‐response pattern at normal diet (blue dots); (ii) proteins with a different fasting response among strains only after 12 weeks of high‐fat diet (green dots); and (iii) proteins with a different fasting response both in normal diet and after 12 weeks of high‐fat diet (red dots). (C) Protein abundance changes in the fed–fasting transition of selected proteins at 0 (T0) and 12 (T2) weeks of high‐fat diet for each mouse strain (B6, blue; S9, pink). Error bars correspond to standard errors. Eduard Sabidó et al. Mol Syst Biol 2013;9:681 © as stated in the article, figure or figure legend