Introduction to the NGC Chromatographic System

Protein purification systems – yesterday & today Simplified, a chromatography system is just: Pumps Valves (Columns) Detector(s) Fraction Collector

NGC System Capabilities Technical Overview Open platform compatible with all medium pressure chromatography columns 10 ml/min or 100 ml/min system pumps Buffer inlet module and buffer blending module for scouting and automated buffer preparation Flexible sample loading options via sample loop (static or dynamic) and a dedicated sample pump all without the need to re-plumb the system, a C96 autosampler can also be connected to the inject valve Column switching module enables up to 5 columns be connected. Contains an internal bypass function for system priming, and reverse flow functionality for affinity chromatography elution and column cleaning Single wavelength detector (255/280nm), or multi-wavelength detector enabling the simultaneous measurement of 4 wavelengths (UV & Vis). Both detectors include an integrated conductivity monitor. pH module with integrated calibration port and bypass port Flexible fraction collection options from 96 well plates, to a range of tubes and large volume bottles Bulletins 6286, 6326, and 6608

A chromatography platform that aligns to your needs Quest™ & Quest™ Plus Workhorse system for the purification of proteins peptides and nucleic acids Scout™ & Scout™ Plus All purpose purification system with buffer scouting Discover ™ Versatile automated purfication system for advanced method development Yes, it does look similar to GE

Expandable system that grows with you Get what you need today with a single, scalable platform that grows and adapts to your throughput and purification needs over time Tier Expansion

Plug-n-Play Modular Design A truly modular design Plug-n-play modules can be easily moved within the system New modules can be added to expand system capabilities as your purification needs change Plug-n-Play Modules Note system must be switched off before modules are removed

ChromLab Software Highlights Touchscreen Intuitive Method Templates Column Library Scouting Multi-D Import GE Unicorn SW and Bio-Rad DuoFlow files Analysis made easy One click peak integration Easy overlay Fraction heat map Concentration determination User Management Edition and Security Edition FREE Bulletin 6675

The Heart of the System - Pumps Two versions available, 10 and 100 mL/min Buffer blending module allows for double flow and simplified buffer preparation ANYONE INTERESTED IN 100 ML/MIN PUMPS IS LIKELY INTERESTED IN BBV – 180 ML/MIN IS PREFERRED OVER 100 ML/MIN BECAUSE YOU ARE NOT REDLINING THE PUMPS – SAME FOR 18 ML/MIN ON 10 ML/MIN PUMPS

Inlets – Great for Automation A buffer inlet valve provides automated buffer switching.

Sample Loading – Volume is Key Volumes >10 ml use a sample loading pump with air sensor. Smaller volumes should be done with loops.

Column Switching Valve Allows reverse flow CSV can also serve as a “loop multiplier” allowing for up to 5 sample loops

Detectors – The “eyes” of the system Single wavelength for labs doing simple protein OR DNA purification Multi-Wavelength is great for labs that: Purify Protein/DNA complexes Purify a fluorescently labeled protein Purify a protein with no signal at 280nm Human Eyes Superman’s Eyes

Outlet – Large Volume Fractionation Useful for labs doing large volume work Able to save the “flow through” in one large container rather using the BioFrac

NGCTM system capabilities System Pump 10 ml/min, 25.2 MPa 100 ml/min, 10 MPa Single-WL Detector 255/280nm BioFracTM Fraction Collector Unlimited Options 180 x 12mm tubes 168 x 1.5ml tubes 120 x 15mm tubes 24 x 30mm tubes 4 x 96, 48-, 24- or 12-position micro-plates 20 x unlimited vol. adaptor Outlet valve Sample Inj. Valve No re-plumbing Column Valve Up to 5 col. Int. Bypass Reverse Flow Pre- & Delta Col. Pressure Conductivity Monitor 0.01 – 999 mS/cm Sample Pump Ideal for Affinity Integrated 100 ml/min, 10 MPa Int. Press. Sensor Buffer Inlet Valve (A/B) Up to 8 Inlets each Multi-WL Detector 190-800nm (UV/Vis) Up to 4 simultaneous 2, 5 & 10 mm Path lengths Buffer Blending Valve pH Scouting Doubles pump flow with gradient Air Sensor Protects Column Up to 8 Inlets (7 Buffers, 1 Sample) pH Probe & Valve In situ calibration Integrated bypass

Standout features Point-to-Plumb™ Pay for only what you need Infinitely configurable Tier expansion Columns mount anywhere Modules can go anywhere Free software—for life! Ten-second module swapping Highly intuitive software