Sequence dependence of Met4 recruitment.

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Sequence dependence of Met4 recruitment. Sequence dependence of Met4 recruitment. (A–E) The median PBM probe fluorescence intensities for GST‐tagged Met4 binding to 685 Met32 sites (A, B) and 673 Cbf1 sites (C–E) in the presence of different 6xHis‐tagged proteins are shown: (A) Met4 binding to Met32 sites assayed in the presence of Met32; (B) Met4 binding to Met32 sites by itself; (C) Met4 binding to Cbf1 sites assayed in the presence of Cbf1; (D) Met4 binding to Cbf1 sites in the presence of Met28; (E) Met4 binding to Cbf1 sites in the presence of Met28 and Cbf1. X‐axis coordinates are the PBM/SPR‐determined Kd values for Met32 and Cbf1 binding to the respective sites. Cartoons in each panel represent the hypothesis being tested. (F) The plot from (E) with Cbf1 sites identified in the promoters of Met4 regulon genes highlighted according to Met4 regulon Class designations of Lee et al (2010) is shown. (G) Ratio of PBM fluorescence values for the Met4/Met28/Cbf1 experiment (E) over the Met4/Cbf1 experiment (C). Individual sites are colored as in (F). Met4 ‘recruitment sites’ are indicated as sites having a ratio >5.0. (H) Overlap of Cbf1 sites identified in upstream promoter region of Met4 regulon genes and Met4 recruitment sites in (G). Promoter regions are defined as 1500 bp upstream of TSS or until next coding region. Significance of observed overlap is calculated using Fisher's one‐tail exact test (hypergeometric distribution). Trevor Siggers et al. Mol Syst Biol 2011;7:555 © as stated in the article, figure or figure legend