HURP and KIF18A do not co-localize on peripheral K-fibers.

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HURP and KIF18A do not co-localize on peripheral K-fibers. HURP and KIF18A do not co-localize on peripheral K-fibers. (A) Shown are example metaphase-arrested cells treated with KIF18A siRNA and transfected with GFP-WT or GFP-sNL1 before fixation and staining with anti-HURP antibodies. Single z-slices of off-axis cells are shown to highlight peripheral K-fibers. White box denotes zoomed region. White dashed lines in zoomed images show line scan locations. Line scans are representative of 19 scans from 8 GFP-WT cells and 21 scans from 9 GFP-sNL1 cells. Green, GFP-KIF18A variant; pink, HURP. See source data for additional line scan examples. (B) Model for the role of KIF18A's neck linker in K-fiber end accumulation. KIF18A WT is ideally suited to navigate obstacles, such as HURP or TACC3, on K-fibers. Conversely, shortening KIF18A's neck linker reduces this navigation ability and limits accumulation to the outside of peripheral K-fibers. KT, kinetochore protein complex. Source data are available for this figure. Heidi LH Malaby et al. LSA 2019;2:e201800169 © 2019 Malaby et al.