ILK knockdown decreases mTOR signaling in PKD kidneys.

Slides:

Advertisements

Similar presentations

From: CXCL10 Is Required to Maintain T-Cell Populations and to Control Parasite Replication during Chronic Ocular Toxoplasmosis Invest. Ophthalmol. Vis.

Advertisements

Prednisone decreases podocyte apoptosis in experimental FSGS

Figure S1 a b c d DAPI Lgr β-catenin Merge

Histomorphology in a case of mixed (cellular and humoral) acute kidney allograft rejection (patient 10). Histomorphology in a case of mixed (cellular and.

Inhibition of ILK by QLT-0267 suppresses type I and type III collagen expression and ameliorates interstitial collagen deposition and renal fibrosis after.

Inhibition of ILK activity does not affect normal kidney structure and nephrin/ILK interaction in vivo. Inhibition of ILK activity does not affect normal.

Impaired lymphatic valve protrusion in Tie2Cre;Piezo1cKO mice.

Klotho reduction causes macrophage invasion into the CP

PD-1 and LAG-3 expression in MSI and MSS colorectal cancer specimens.

Sunitinib plus rMVA–CEA–TRICOM vaccine decreased tumor burden and increased intratumoral infiltration of T lymphocytes in the MC38-CEA colon carcinoma.

INT-767 treatment prevents the increase in renal inflammation and oxidative stress in diabetic DBA/2J mice. INT-767 treatment prevents the increase in.

Expression of IL-1α in goblet cells and their destruction during DSS-induced colitis. Expression of IL-1α in goblet cells and their destruction during.

INT-767 treatment prevents renal lipid accumulation in diabetic DBA/2J mice. INT-767 treatment prevents renal lipid accumulation in diabetic DBA/2J mice.

Ang II induces translocation of α-ENaC toward the apical membrane.

Renal microvascular injury 6 months after induction of JG cell-specific Gsα knockout. Renal microvascular injury 6 months after induction of JG cell-specific.

Fluorescence-stained images and respective bright-field images of mouse spleen tissue sections area investigated using Raman spectroscopy. Fluorescence-stained.

Pioglitazone protects against aging-related renal injury.

Alveolar lymphangiogenesis is a feature of idiopathic pulmonary fibrosis (IPF). a) Tissue sections reacted with anti-D2-40 (brown) and anti-CD34 (red)

(red) & endothelium (green) PMCA4 (green) & DAPI (blue)

Detection and tracking of individual SNAP-tagged proteins on the surface of living cells. Detection and tracking of individual SNAP-tagged proteins on.

Yongji Wang, Megan L. Borchert, Hector F. DeLuca Kidney International

CD169+ macrophages mediate Lm translocation to the splenic T cell zones. CD169+ macrophages mediate Lm translocation to the splenic T cell zones. (A) Confocal.

Elysse C. Filipe et al. BTS 2018;3:38-53

Isabelle Plaisance et al. BTS 2016;j.jacbts

KIF1Bβ promotes DHX9 nuclear localization.

Marker identification and quantification by StrataQuest, and confocal analysis of EV-containing tissue sections. Marker identification and quantification.

The increased cytosolic and nuclear β-catenin in the tubular epithelial cells of 7-d obstructed kidneys is suppressed by recombinant sFRP4. The increased.

Inhibition of VEGFA or macrophage-specific ablation of Vegfa from TIE2hi/VEGFAhi TMEM macrophages reduces vascular permeability and tumor cell intravasation.

Only anti-CD133 antibodies recognizing the CD133/1 or the CD133/2 epitopes can identify human renal progenitors Maria L. Angelotti, Elena Lazzeri, Laura.

Effectively regenerating livers transiently accumulate proliferative IGF2BP3-positive cells. Effectively regenerating livers transiently accumulate proliferative.

Fig. 3 CSF1 is expressed in human melanoma.

B cells infiltrate mouse and human pancreatic neoplasia and promote growth of KrasG12D-PDEC in vivo. B cells infiltrate mouse and human pancreatic neoplasia.

HDAC4N increases with Braak stage in CA2 hippocampal pyramidal cells.

Synaptopodin expression is also in cells on Bowman’s capsule in juvenile human kidney. Synaptopodin expression is also in cells on Bowman’s capsule in.

Wild-type PC1 promotes ciliary localization of endogenous PC2 in ADPKD hepatoblasts. Wild-type PC1 promotes ciliary localization of endogenous PC2 in ADPKD.

ILK knockdown increases the survival of PKD mice.

VEGFC treatment improves kidney histology and survival in Cys1cpk/cpk mice. VEGFC treatment improves kidney histology and survival in Cys1cpk/cpk mice.

PINCH-1 forms a complex with integrin-linked kinase (ILK) at focal adhesion sites. PINCH-1 forms a complex with integrin-linked kinase (ILK) at focal adhesion.

Representative photomicrographs of immunohistochemical staining for Wilms’ tumor 1 (WT1) and cleaved caspase-3 in mouse kidney sections at 10, 20, and.

Distribution of splenic T cells, B cells, and NK cells in NSG hL-7xhIL-15 humanized mice. Distribution of splenic T cells, B cells, and NK cells in NSG.

Neutralization and knockdown of TGFβ2 results in fewer nephrons ex vivo. Neutralization and knockdown of TGFβ2 results in fewer nephrons ex vivo. (A,B)

Detection of nuclear foci by FISH on frozen sections of diaphragm muscle prepared from 2-month-old DMSXL mice. Detection of nuclear foci by FISH on frozen.

Fig. 2. Sufficient rate of recombination in embryonic NSCs and NPCs of Emx1cre knock-in mice.(A) Breeding schemes to generate mice with the Emx1cre locus.

Significant pathological changes and activation of Nrf2 pathway in the glomeruli of human diabetic nephropathy patients. Significant pathological changes.

Nerve conduction slowing and DRG sensory neuron and nuclear atrophy in 16-week-old diabetic mice. Nerve conduction slowing and DRG sensory neuron and nuclear.

CD169+ macrophages mediate the transport of bacteria to T cell zones by trans-infecting CD8α+ DCs. CD169+ macrophages mediate the transport of bacteria.

Exo70 is recruited to the plasma membrane at sites of mechanical wounding. Exo70 is recruited to the plasma membrane at sites of mechanical wounding. NRK.

Gα12 is not required for hepatic cystogenosis induced by Pkd1 knockout

DNM inhibition affects ITGB1 activity and Rab11 cell distribution in human MKs. (A-B) Quantification of active ITGB1 at the surface of MKs. Representative.

Prophase nuclear movements in wild-type, rec8 and rec7 mutant cells.

Characterization of NEX-CRE mice and analysis of Itgb1 expression by flow cytometry. a–n , Z/EG reporter mice carrying a CRE-inducible GFP transgene were.

CENP-F staining in prometaphase and metaphase spermatocytes from B6 and Rb/+ mice. CENP-F staining in prometaphase and metaphase spermatocytes from B6.

Histone crotonylation in kidney tubular cells.

The absence of host Fbln5 results in increased apoptosis and decreased proliferation in Pan02 tumors. The absence of host Fbln5 results in increased apoptosis.

Detection of phosphorylated Smad2/3 (pSmad2/3) in the skin of wild-type and transgenic animals during the first hair cycle. Detection of phosphorylated.

PTZ-induced c-Fos and Arc proteins in hippocampal dentate gyrus

IKKβ protects adipocytes from HFD-induced cell death.

Identification of a novel subset of mDA neurons in the VTA that expresses Neurod6, OTX2, CALBINDIN1, ALDH1A1, and Grp. Identification of a novel subset.

Proprioceptive sensory neurons and muscle spindles are marked reduced in adult Swl/+ mice. Proprioceptive sensory neurons and muscle spindles are marked.

In vivo thrombosis of tumor vasculature.

A His/Pro-rich fragment of HRG is present in human tissue.

Fig. 2 IRF8 is expressed in CD68+ macrophages after SCI.

Expression of XCR1 in normal ovarian surface epithelium and the IOSE and borderline EOC cell lines. Expression of XCR1 in normal ovarian surface epithelium.

Β-catenin knockout inhibits synovial sarcoma (SS) tumor growth in SYT–SSX2 transgenic mice. β-catenin knockout inhibits synovial sarcoma (SS) tumor growth.

PC loss in Nhe6-null mouse cerebellum, female and male.

Expression of PCNA, K10, and K5 in skin lesions from Stat3+/−:HPV8 and Stat3+/+:HPV8 mice. Expression of PCNA, K10, and K5 in skin lesions from Stat3+/−:HPV8.

Expression of Rapgef2 and Rapgef6 in the E13.5 and E15.5 brains.

Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization in 231BR3 cells. Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization.

CD36 expression in tissue adjacent and distal to the tumor.

PEGPH20 depletes HA and decompresses intratumoral vessels.

Presentation transcript:

ILK knockdown decreases mTOR signaling in PKD kidneys. ILK knockdown decreases mTOR signaling in PKD kidneys. Representative sections from (A) WT, (B) Ilk+/+;Pkd1fl/fl;Pkhd1-Cre (Ilk+/+ PKD), (C) Ilkfl/+;Pkd1fl/fl;Pkhd1-Cre (Ilk+/−PKD), and (D) Ilkfl/fl;Pkd1fl/fl;Pkhd1-Cre (Ilk−/− PKD) mice were stained with an antibody to P-S6 (red). Tissues were also stained for DBA (green) to detect CD and the DAPI nuclear stain (blue). Arrows indicate cytoplasmic P-S6 in CD cells. All images were taken at the same magnification. Scale bar=5 µm. (E) Percentage of cytosolic P-S6–positive cells, normalized to the total nuclei in DBA-positive CDs. Data are mean±SEM for kidneys from three mice per group. **P<0.01 compared with WT, ##P<0.01 compared with Ilk+/+ PKD. Archana Raman et al. JASN 2017;28:2708-2719 ©2017 by American Society of Nephrology