Transfection of stable β-catenin (S33Y) increased nuclear β-catenin and phosphorylated Akt expression (A) and reduced the promoting effect of HG on caspase-3.

Slides:

Advertisements

Similar presentations

PP1 and PP2A inhibitors rescue α-SMA expression and Akt phosphorylation from the inhibitory effects of TSA. After serum starvation, NHLFs were pretreated.

Advertisements

Src-Family Kinases Are Activated in Non-Small Cell Lung Cancer and Promote the Survival of Epidermal Growth Factor Receptor-Dependent Cell Lines Jie.

by Kumudha Balakrishnan, William G. Wierda, Michael J

Downregulation of TRPC6 expression by sildenafil leads to reduced TRPC6–mediated Ca2+ influx into the podocyte. Downregulation of TRPC6 expression by sildenafil.

Volume 69, Issue 9, Pages (May 2006)

IL-2–mediated apoptosis of kidney tubular epithelial cells is regulated by the caspase-8 inhibitor c-FLIP Caigan Du, Qiunong Guan, Ziqin Yin, Robert.

Reduced cell motility and expression of phosphorylated FAK in a fibroblast cell line of an individual with T3257I mutation. Reduced cell motility and expression.

ARG tyrosine kinase activity is inhibited by STI571

Small-molecule inhibitor QLT-0267 suppresses ILK activity and inhibits its downstream signaling. Small-molecule inhibitor QLT-0267 suppresses ILK activity.

Both patient 1 and her family members showed a normal physiological AQP2 response to DDAVP. Immunoblotting for AQP2 was performed on urinary exosomes derived.

T cell receptor Vβ (TCR-Vβ) expression in a drug-specific T cell line (TCL) and specificity assay of a TCL of patient P1. T cell receptor Vβ (TCR-Vβ) expression.

The selective progesterone receptor modulator CDB4124 inhibits proliferation and induces apoptosis in uterine leiomyoma cells Xia Luo, Ph.D., Ping Yin,

a b MCF-7 TR2 MCF-7 TR2 (Fold change) MTT Assay , (Fold change)

Volume 60, Issue 2, Pages (August 2001)

L.-H. Weng, C.-J. Wang, J.-Y. Ko, Y.-C. Sun, Y.-S. Su, F.-S. Wang

SEMA3C silencing inhibits CRPC cell growth and induces apoptosis

MCF7z.p85WT MCF7z.p85S83μ MCF7z.p85S83A MCF7.vec MCF7z P-Akt T-Akt

NM-3 induces p21 levels and down-regulation of Cdk2 activity.

The increased cytosolic and nuclear β-catenin in the tubular epithelial cells of 7-d obstructed kidneys is suppressed by recombinant sFRP4. The increased.

Involvement of PI3K activation in TCR/CD3-dependent HIF-1α protein expression. Involvement of PI3K activation in TCR/CD3-dependent HIF-1α protein expression.

Volume 62, Issue 4, Pages (October 2002)

Box plots showing the relative difference in distribution of mtDNA copy number per cell between patients with ESRD and healthy control subjects frequency.

Representative photomicrographs of immunohistochemical staining for Wilms’ tumor 1 (WT1) and cleaved caspase-3 in mouse kidney sections at 10, 20, and.

Association of endogenous cadherins with CDO and BOC

Romidepsin reduces histone deacetylase activity, induces acetylation of histones, inhibits proliferation, and activates apoptosis in immortalized epithelial.

TC-1 silencing sensitized A549 and SPC-A-1 cells to radiation therapy

Fig. 3. Inactivation of the Wnt/β-catenin signaling pathway inhibited cell proliferation and induced apoptosis in A549 and SPC-A-1 cells. Inactivation.

Ectopic expression of wt-DAO, but not the inactive mutant, promotes senescence. Ectopic expression of wt-DAO, but not the inactive mutant, promotes senescence.

RXRa serves as a carrier for TR3 translocation initiated by 9-cis retinoic acid. RXRa serves as a carrier for TR3 translocation initiated by 9-cis retinoic.

JAK3A572V mutation causes constitutive JAK3 activity and IL-2–independent proliferation of NKTCL cells. JAK3A572V mutation causes constitutive JAK3 activity.

Cell proliferations, ROS production, and ATP levels in HASMCs cultured under high-glucose condition. Cell proliferations, ROS production, and ATP levels.

MYC is a key determinant of cancer cell survival under p300- or CBP-depletion in CBP-KO or p300-KO cells, respectively. MYC is a key determinant of cancer.

Caspase activation occurs after AIF translocation and nuclear condensation. Caspase activation occurs after AIF translocation and nuclear condensation.

The selective progesterone receptor modulator CDB4124 inhibits proliferation and induces apoptosis in uterine leiomyoma cells Xia Luo, Ph.D., Ping Yin,

The wild-type BRCA1-BARD1 EM structure resembles a clamp-like motif

Class 1 phosphatidylinositol 3-kinase (PI3K) p85 subunit dominant negative adenovirus regulates Pax2 and GAPDH abundance in NRK-52E cells. Class 1 phosphatidylinositol.

eNOS regulated IR-induced NO generation and EGFR signal activation.

Effect of GSK-3β inhibition on cell-confluence-induced apoptosis.

The P450scc construct increases functional P450scc expression resulting in elevated pregnenolone levels. The P450scc construct increases functional P450scc.

Fig. 6 RUNX/CBFB interaction inhibitor, Ro5-3335, significantly decreases mouse neurofibroma growth in vivo. RUNX/CBFB interaction inhibitor, Ro5-3335,

The effects of the FASN blocker C75 on cell growth and survival (A, E, and F), on expression/phosphorylation of PI3K and MAPK signaling proteins and on.

Β-Catenin regulation of AChR clustering requires interaction with α-catenin. β-Catenin regulation of AChR clustering requires interaction with α-catenin.

Aggregation and toxicity of α-synuclein in HEK293T cells.

Fig. 4. Effects of natriuretic peptide receptor 2 (NPR2) knockdown on cells apoptosis in mouse Leydig cells. (A) Relative mRNA expression of cell apoptosis.

A, IGFBP-3 knockdown via siRNA using real-time qRT-PCR analysis.

Induction of CDCP1 is regulated by Ras in NSCLC cells.

Imatinib mesylate inhibits PDGF-mediated ERK and Akt activation.

Lamellarin D induces cell death through a Fas-independent pathway.

Down-regulation of the erbB-2 receptor by trastuzumab decreases Akt kinase activation but not MAPK activation. Down-regulation of the erbB-2 receptor by.

CXCR4 expression levels of MDA-MB-231 cells at 48 hours posttransfection of CXCR4 siRNAs. CXCR4 expression levels of MDA-MB-231 cells at 48 hours posttransfection.

Effects of visfatin on the cell proliferation and phosphorylation of ERK, Akt, and GSK-3β proteins in HCC cells. Effects of visfatin on the cell proliferation.

Expression of versican promoted breast cancer cell self-renewal through enhanced EGFR/AKT/GSK-3β (S9P) signals. Expression of versican promoted breast.

Curcumin suppresses the expression of antiapoptotic proteins in multiple myeloma cells. Curcumin suppresses the expression of antiapoptotic proteins in.

A. A. Honokiol inhibits TNF-induced NF-κB activation, IκBα phosphorylation, and IκBα degradation. Honokiol inhibits TNF-induced activation of NF-κB. H1299.

GPC3 promotes hepatocellular carcinoma growth by stimulating the canonical Wnt pathway (A-B) GPC3 induces the stabilization of cytoplasmic β-catenin. GPC3.

ERK reactivation following EGFR TKI treatment.

MDM2 Protein Overexpression Inhibits Apoptosis of TF-1 Granulocyte-Macrophage Colony-Stimulating Factor–Dependent Acute Myeloblastic Leukemia Cells by.

WP1066 induces caspase-dependent apoptosis.

ODC protein expression in paired benign and cancer tissue obtained from patients with PCA. The protein expression was measured by immunoblot analysis in.

TBK1 signaling also promotes basal autophagy in 8988T cells.

Expression of HIF-1α and β-catenin in hypoxic HCC cell lines and HAL-treated xenografts. Expression of HIF-1α and β-catenin in hypoxic HCC cell lines and.

Induction of PARP cleavage (A) and activation of caspases (B) after treatment with a combination of TRAIL and cisplatin. Induction of PARP cleavage (A)

W. chinensis extract induces apoptosis in AR-dependent prostate cancer cells. W. chinensis extract induces apoptosis in AR-dependent prostate cancer cells.

RA-9 induces G2–M cell-cycle arrest and caspase-mediated apoptosis in ovarian cancer cells. RA-9 induces G2–M cell-cycle arrest and caspase-mediated apoptosis.

High nuclear β-catenin levels confer resistance to AKT inhibition and coordinates with increased nuclear FOXO3a to promote metastasis in colon cancer.

LY elicits hyperphosphorylation of Akt.

Mechanism by which flavopiridol induces cell cycle arrest and apoptosis in rhabdoid tumor cells. Mechanism by which flavopiridol induces cell cycle arrest.

Depletion of murine Dnmt proteins after 5-Aza-CdR treatment.

Knockdown of NR4A1 induces endoplasmic reticulum stress–mediated apoptosis by increasing ROS production in pancreatic cancer cells. Knockdown of NR4A1.

D-GM3 promotes uPAR clustering on the cell surface and activates p38 MAPK. A, uPAR expression in cells was either knocked down by treatment with 4 independent.

Presentation transcript:

Transfection of stable β-catenin (S33Y) increased nuclear β-catenin and phosphorylated Akt expression (A) and reduced the promoting effect of HG on caspase-3 and PARP activation (B), cell apoptosis (C), and reversed cell proliferation (D). Transfection of stable β-catenin (S33Y) increased nuclear β-catenin and phosphorylated Akt expression (A) and reduced the promoting effect of HG on caspase-3 and PARP activation (B), cell apoptosis (C), and reversed cell proliferation (D). Mesangial cells were subjected to transfection with wild-type or stable β-catenin cDNA. Stably transfected cell cultures were subjected to HG stress for 48 h. Immunoblotting of total caspase-3 and actin showed equal loadings and transfer for all lanes. P < 0.05, *vehicle and #HG-treated groups. Chun-Liang Lin et al. JASN 2006;17:2812-2820 ©2006 by American Society of Nephrology