Quantification of MHC-I, β2m, and T-cell subsets.

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Quantification of MHC-I, β2m, and T-cell subsets. Quantification of MHC-I, β2m, and T-cell subsets. A, representative staining in triplicate of MHC-I expression in 5 patients with CLM, with calculated mean percentage surface expression ± SE. For the same tumors, example of β2m staining of one of the triplicate cores is shown. Correlation of MHC-I and β2m expression is shown. Solid and dotted lines represent median and terciles, respectively (MHC-I, 32%, 48%, and 65%; β2m, 13%, 21%, and 33%). B, quantification of intratumoral CD3 (n = 154), CD4 (n = 155), and CD8 (n = 155) T cells. One dot represents one metastasis (cells/core, mean of replicates). Bars represent medians (109, 9, and 52 cells/core, respectively). One value is out of scale (CD8 = 502). C, within the 52 tumors found to express the highest level of MHC-I (upper tercile), 31 displayed high CD3 infiltration, 80.6% of which represented tumors detected to have high CD4 and/or CD8 infiltration (25 of 31, medians used as cutoff points). D, dot plot representing the absence of a correlation between MHC-I expression and CD3 infiltration. Using the highest tercile as cutoff for high MHC-I expression (broken Y-axis, 65%) and the median count for high CD3 infiltration (broken X-axis, 109 cells/core), four groups are defined: MHC-IhiCD3hi (n = 31, red); MHC-IloCD3hi (n = 46, blue); MHC-IhiCD3lo (n = 19, yellow); and MHC-IloCD3lo (n = 58, black). Spearman r used for correlation analysis. Simon Turcotte et al. Cancer Immunol Res 2014;2:530-537 ©2014 by American Association for Cancer Research