Ciliary abnormalities of jck renal epithelia.

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Ciliary abnormalities of jck renal epithelia. Ciliary abnormalities of jck renal epithelia. (A) Real-time PCR analysis of Nek8, Pkd1, and Pkd2 mRNA expression in WT and jck kidney. The expression levels were normalized to Rps12 housekeeping gene. The results are shown as the mean and SD of three different animals. (B) Differential ciliary distribution of Nek8 and polycystins in jck and WT renal epithelia. The cilia are marked with antibody to acetylated tubulin (ac. tubulin). Note punctate ciliary expression of Nek8 in WT epithelia and loss of ciliary localization in cystic epithelial cells. Polycystin-1 (PC-1) and PC-2 are expressed mainly at the base of cilia in WT cells, whereas their expression is enhanced along the cilia in jck cells. (C) Scanning electron micrographs of renal epithelial cells in WT and jck kidney. Primary cilia are significantly lengthened in cystic kidneys. Laurie A. Smith et al. JASN 2006;17:2821-2831 ©2006 by American Society of Nephrology