Luteinizing hormone depletes ascorbic acid in preovulatory follicles

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Luteinizing hormone depletes ascorbic acid in preovulatory follicles Michael M Guarnaccia, M.D., M Takami, M.D., Ervin E Jones, Ph.D., M.D., Sandra L Preston, M.S., Harold R Behrman, Ph.D. Fertility and Sterility Volume 74, Issue 5, Pages 959-963 (November 2000) DOI: 10.1016/S0015-0282(00)01550-8

Figure 1 Depletion of ascorbic acid in ovaries of preovulatory rats. LH or saline was injected at 10:00 a.m. into rats and the ovaries removed at the indicated times for analysis of ascorbic acid content (see Materials and Methods). The 0-h control group represents saline-injected animals from which ovaries were removed 1 h after injection. Mean ± SEM (n = 5 animals per group). ∗P<.05. Guamaccia. LH depletes follicle ascorbic acid. Fertil Steril 2000. Fertility and Sterility 2000 74, 959-963DOI: (10.1016/S0015-0282(00)01550-8)

Figure 2 Depletion of ascorbic acid in preovulatory follicles. LH or saline was injected at 10:00 a.m. into rats, the ovaries were removed, and the preovulatory follicles dissected on an ice-cold microscope stage, pooled, and frozen for later analysis of ascorbic acid (see Materials and Methods). The 0-h control group represents saline-treated rats killed immediately after injection. ∗P<.05 (vs. 0 h). Guamaccia. LH depletes follicle ascorbic acid. Fertil Steril 2000. Fertility and Sterility 2000 74, 959-963DOI: (10.1016/S0015-0282(00)01550-8)

Figure 3 Depletion of ascorbic acid in isolated follicles by LH and menadione. Preovulatory follicles were isolated and incubated with either hCG (20 IU/mL) or menadione (mendione; 100 μM) for 3 h (see Material and Methods). Follicles were removed and the ascorbic acid content determined. ∗P<.05 (vs. control). Guamaccia. LH depletes follicle ascorbic acid. Fertil Steril 2000. Fertility and Sterility 2000 74, 959-963DOI: (10.1016/S0015-0282(00)01550-8)

Figure 4 Oocyte ascorbic acid accumulation by cumulus-enclosed and denuded oocytes. Denuded oocytes (DO) or cumulus-enclosed oocytes (CEO) were isolated and incubated in the absence (open bars) or presence (solid bars) of ascorbic acid (150 μM) for 3 h, and the oocytes were subsequently isolated from OCC under ice-cold conditions before analysis by HPLC (see Materials and Methods). Mean ± SEM of 3 experiments. ND = not detectable. Guamaccia. LH depletes follicle ascorbic acid. Fertil Steril 2000. Fertility and Sterility 2000 74, 959-963DOI: (10.1016/S0015-0282(00)01550-8)