Quantitative real-time PCR and the (1 → 3)-β-d-glucan assay for differentiation between Pneumocystis jirovecii pneumonia and colonization  Y. Matsumura,

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Quantitative real-time PCR and the (1 → 3)-β-d-glucan assay for differentiation between Pneumocystis jirovecii pneumonia and colonization  Y. Matsumura, Y. Ito, Y. Iinuma, K. Yasuma, M. Yamamoto, A. Matsushima, M. Nagao, S. Takakura, S. Ichiyama  Clinical Microbiology and Infection  Volume 18, Issue 6, Pages 591-597 (June 2012) DOI: 10.1111/j.1469-0691.2011.03605.x Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 P. jirovecii copy numbers (a) and β-d-glucan levels (b) according to patient group. In the box and whisker plots, the boxes contain 50% of the sample data, with the median value indicated by a horizontal bar. The whiskers contain 1.5 × the interquartile range. (a) The copy numbers were significantly higher in the definite PCP group when compared with the colonized group in both BAL and IS samples. The copy numbers were not significantly different between the BAL and IS samples in the definite and probable PCP groups (p 0.57 and p 0.29), but were significantly different in the colonized group (p 0.02). (b) The β-d-glucan levels were significantly higher in both the definite and probable PCP groups when compared with the colonized group. Clinical Microbiology and Infection 2012 18, 591-597DOI: (10.1111/j.1469-0691.2011.03605.x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Receiver-operator characteristic curves of P. jirovecii copy numbers and β- d-glucan levels for the diagnosis of PCP. (a) The definite PCP group was compared with the colonized group. The arrows indicate cut-off values of 15.6 pg/mL for β-d-glucan level and 1300 copies/mL for copy number. (b) The probable PCP group was compared with the colonized group. The arrows indicate cut-off values of 6.0 pg/mL for β-d-glucan level and 340 copies/mL for copy number. Clinical Microbiology and Infection 2012 18, 591-597DOI: (10.1111/j.1469-0691.2011.03605.x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions