Quantitative real-time PCR and the (1 → 3)-β-d-glucan assay for differentiation between Pneumocystis jirovecii pneumonia and colonization Y. Matsumura,

Slides:

Advertisements

Similar presentations

Models for the organisation of hospital infection control and prevention programmes B. Gordts Clinical Microbiology and Infection Volume 11, Pages

Advertisements

Laboratory diagnosis and biosafety issues of biological warfare agents

The plasma level of soluble urokinase receptor is elevated in patients with Streptococcus pneumoniae bacteraemia and predicts mortality P. Wittenhagen,

C.-S. Lee, J.-H. Lee Clinical Microbiology and Infection

P.-Y. Lévy Clinical Microbiology and Infection

Detection of intestinal protozoa in paediatric patients with gastrointestinal symptoms by multiplex real-time PCR L. Maas, J.W. Dorigo-Zetsma, C.J. de.

R. Dumke, H. von Baum, P.C. Lück, E. Jacobs

L. Boyanova Clinical Microbiology and Infection

C. Steininger, M. Redlberger, W. Graninger, M. Kundi, T. Popow-Kraupp

Lipooligosaccharide locus class of Campylobacter jejuni: sialylation is not needed for invasive infection P. Ellström, B. Feodoroff, M.-L. Hänninen,

High sensitivity and specificity of the C6-peptide ELISA on cerebrospinal fluid in Lyme neuroborreliosis patients N.D. van Burgel, A. Brandenburg, H.J.

Gut bacterial microbiota and obesity

Approach to diagnosis of infective endocarditis

C-reactive protein as a marker of infection in critically ill patients

A.G. Koek, S.M. Bruisten, M. Dierdorp, A.P. van Dam, K. Templeton

Mycobacterial peritonitis: difference between non-tuberculous mycobacteria and Mycobacterium tuberculosis C.-C. Shu, J.-T. Wang, J.-Y. Wang, C.-J. Yu,

E. Bronska, J. Kalmusova, O. Dzupova, V. Maresova, P. Kriz, J. Benes

C.-S. Lee, J.-H. Lee Clinical Microbiology and Infection

Clinical characteristics, outcome and the role of viral load in nontransplant patients with Epstein--Barr viraemia T. Martelius, V.-J. Anttila Clinical.

Development of a real-time PCR targeting the yidC gene for the detection of Mycoplasma hominis and comparison with quantitative culture C. Férandon,

Real-time PCR assay-based strategy for differentiation between active Pneumocystis jirovecii pneumonia and colonization in immunocompromised patients

R. Cantón Clinical Microbiology and Infection

Performance of Focus ELISA tests for herpes simplex virus type 1 (HSV-1) and HSV-2 antibodies among women in ten diverse geographical locations R. Ashley-Morrow,

A prospective comparison of galactomannan in bronchoalveolar lavage fluid for the diagnosis of pulmonary invasive aspergillosis in medical patients under.

An eye-catching acanthocephalan

Comparison of microscopy, real-time PCR and a rapid immunoassay for the detection of Giardia lamblia in human stool specimens T. Schuurman, P. Lankamp,

Potential risk factors for infection with Candida spp

Climate and genotypes of Pneumocystis jirovecii

Vector control: a cornerstone in the malaria elimination campaign

Sofie Arens, Jan Verhaegen, Ludo Verbist

Pneumocystis jirovecii multilocus genotyping in pooled DNA samples: a new approach for clinical and epidemiological studies F. Esteves, J. Gaspar, B.

Leucocyte esterase, glucose and C-reactive protein in the diagnosis of prosthetic joint infections: a prospective study E. De Vecchi, F. Villa, M. Bortolin,

Detection of human metapneumovirus and respiratory syncytial virus by duplex real-time RT-PCR assay in comparison with direct fluorescent assay P. Jokela,

Laboratory diagnosis and biosafety issues of biological warfare agents

CMI editorial report 2011 Clinical Microbiology and Infection

Detection of interleukin-2 in addition to interferon-γ discriminates active tuberculosis patients, latently infected individuals, and controls R. Biselli,

Clinical and microbiological characteristics of Nocardiosis including those caused by emerging Nocardia species in Taiwan, 1998–2008 C.-K. Tan, C.-C.

Elements of design: the knowledge on which we build

Levofloxacin in the treatment of ventilator-associated pneumonia

Close cooperation between infectious disease physicians and attending physicians can result in better management and outcome for patients with Staphylococcus.

D. E. Karageorgopoulos, J. -M. Qu, I. P. Korbila, Y. -G. Zhu, V. A

Metagenomics and probiotics

Laboratory diagnosis of Clostridium difficile disease

J. Garau Clinical Microbiology and Infection

T.M. File Clinical Microbiology and Infection

Interference between respiratory syncytial virus and rhinovirus in respiratory tract infections in children S. Karppinen, L. Toivonen, L. Schuez-Havupalo,

Antibacterial drug discovery in the 21st century

Community-acquired pneumonia: epidemiologic and clinical consideration

Abstracts cont. Clinical Microbiology and Infection

N. Jazmati, M. Hellmich, B. Ličanin, G. Plum, A.J. Kaasch

Evaluation of eight commercial tests for Mycoplasma pneumoniae antibodies in the absence of acute infection R. Nir-Paz, A. Michael-Gayego, M. Ron, C.

Real-time PCR assays compared to culture-based approaches for identification of aerobic bacteria in chronic wounds J.H. Melendez, Y.M. Frankel, A.T.

H. Leblebicioglu, C. Eroglu Clinical Microbiology and Infection

Correlation between time to positivity of blood cultures with clinical presentation and outcomes in patients with Klebsiella pneumoniae bacteraemia: prospective.

Statin use and clinical outcomes among pneumonia patients

Clinical Microbiology and Infection

European guidelines for diagnosis and management of patients with suspected herpes simplex encephalitis Miles Denton, Kevin G Kerr, Fraser Lewis Clinical.

The atypical pneumonias: clinical diagnosis and importance

J.J. Keller, M.-C. Tsai, C.-C. Lin, Y.-C. Lin, H.-C. Lin

Outcome of prosthetic knee-associated infection: evaluation of 40 consecutive episodes at a single centre R.R. Laffer, P. Graber, P.E. Ochsner, W. Zimmerli

Abstracts Clinical Microbiology and Infection

Modelling during an emergency: the 2009 H1N1 influenza pandemic

Usefulness of a quantitative real-time PCR assay using serum samples to discriminate between inactive, serologically positive and active human brucellosis

J.L. Balcázar Clinical Microbiology and Infection

Three years experience of real-time PCR for the diagnosis of Q fever

J.A. Pérez-Molina, F. Fernández Clinical Microbiology and Infection

Impact of antibiotic restrictions: the patient's perspective

High prevalence of mutators among Enterobacter cloacae nosocomial isolates and their association with antimicrobial resistance and repetitive detection

CMI readers' survey Clinical Microbiology and Infection

The future of diagnostic bacteriology

Presentation transcript:

Quantitative real-time PCR and the (1 → 3)-β-d-glucan assay for differentiation between Pneumocystis jirovecii pneumonia and colonization Y. Matsumura, Y. Ito, Y. Iinuma, K. Yasuma, M. Yamamoto, A. Matsushima, M. Nagao, S. Takakura, S. Ichiyama Clinical Microbiology and Infection Volume 18, Issue 6, Pages 591-597 (June 2012) DOI: 10.1111/j.1469-0691.2011.03605.x Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 P. jirovecii copy numbers (a) and β-d-glucan levels (b) according to patient group. In the box and whisker plots, the boxes contain 50% of the sample data, with the median value indicated by a horizontal bar. The whiskers contain 1.5 × the interquartile range. (a) The copy numbers were significantly higher in the definite PCP group when compared with the colonized group in both BAL and IS samples. The copy numbers were not significantly different between the BAL and IS samples in the definite and probable PCP groups (p 0.57 and p 0.29), but were significantly different in the colonized group (p 0.02). (b) The β-d-glucan levels were significantly higher in both the definite and probable PCP groups when compared with the colonized group. Clinical Microbiology and Infection 2012 18, 591-597DOI: (10.1111/j.1469-0691.2011.03605.x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Receiver-operator characteristic curves of P. jirovecii copy numbers and β- d-glucan levels for the diagnosis of PCP. (a) The definite PCP group was compared with the colonized group. The arrows indicate cut-off values of 15.6 pg/mL for β-d-glucan level and 1300 copies/mL for copy number. (b) The probable PCP group was compared with the colonized group. The arrows indicate cut-off values of 6.0 pg/mL for β-d-glucan level and 340 copies/mL for copy number. Clinical Microbiology and Infection 2012 18, 591-597DOI: (10.1111/j.1469-0691.2011.03605.x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions