Protective effect of 20-hydroxyeicosatetraenoic acid (20-HETE) on glomerular protein permeability barrier Ellen T. Mccarthy, R.A.M. Sharma, Mukut Sharma,

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Presentation transcript:

Protective effect of 20-hydroxyeicosatetraenoic acid (20-HETE) on glomerular protein permeability barrier Ellen T. Mccarthy, R.A.M. Sharma, Mukut Sharma, M.D. Kidney International Volume 67, Issue 1, Pages 152-156 (January 2005) DOI: 10.1111/j.1523-1755.2005.00065.x Copyright © 2005 International Society of Nephrology Terms and Conditions

Figure 1 Puromycin aminonucleoside (PAN) increases Palb of isolated glomeruli. Isolated glomeruli were incubated with PAN (5μg/mL) for various time periods, and Palb was measured. Values for Palb are expressed as mean ± SEM (N = number of glomeruli). *P < 0.05 vs. control; **P < 0.01 vs. control; #P < 0.001 vs. control. Palb at 15 minutes vs. 30 and 60 minutes = NS. Kidney International 2005 67, 152-156DOI: (10.1111/j.1523-1755.2005.00065.x) Copyright © 2005 International Society of Nephrology Terms and Conditions

Figure 2 20-hydroxyeicosatetraenoic acid (20-HETE) protects glomerular permeability barrier from increased Palb caused by puromycin aminonucleoside (PAN). Isolated glomeruli were preincubated with 20-HETE (10 to 100 nmol/L) followed by further incubation for 15 minutes after adding PAN (5μg/mL), and Palb was measured. Values for Palb are expressed as mean ± SEM (N = number of glomeruli). #P < 0.05 vs. PAN; *P < 0.02 vs. PAN. Kidney International 2005 67, 152-156DOI: (10.1111/j.1523-1755.2005.00065.x) Copyright © 2005 International Society of Nephrology Terms and Conditions

Figure 3 Glomeruli from rats treated with clofibrate are protected from increased Palb caused by puromycin aminonucleoside (PAN). Rats were treated with clofibrate (500mg/kg/day, intraperitoneally) or vehicle (olive oil, 1mL/kg/day, intraperitoneally) for 3 days. Glomeruli were isolated and incubated with PAN (5μg/mL) or saline for 15 minutes. Values for Palb are expressed as mean ± SEM (N = number of glomeruli). *P < 0.001 vs. control. Kidney International 2005 67, 152-156DOI: (10.1111/j.1523-1755.2005.00065.x) Copyright © 2005 International Society of Nephrology Terms and Conditions

Figure 4 Glomeruli from rats treated with clofibrate show increased expression of cytochrome P450 (CYP450 4A). Experimental rats were treated with clofibrate (500mg/kg/day, intraperitoneally) and control rats with vehicle (olive oil, 1mL/kg/day, intraperitoneally) for 3 days. Isolated glomeruli were homogenized in a lysis buffer. P450 4A expression was determined by Western blot analysis as described in the Methods section. Kidney International 2005 67, 152-156DOI: (10.1111/j.1523-1755.2005.00065.x) Copyright © 2005 International Society of Nephrology Terms and Conditions