Potential role of vacuolar H+–adenosine triphosphatase in neointimal formation in cultured human saphenous vein Hajime Otani, MDa, Hideyasu Ohmiya, MDa,

Slides:

Advertisements

Similar presentations

Orally administered penicillamine is a potent inhibitor of neointimal and medial thickening in porcine saphenous vein–carotid artery interposition grafts

Advertisements

Expression of molecular mediators of apoptosis and their role in the pathogenesis of lower-extremity varicose veins Enrico Ascher, MD, Theresa Jacob,

Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff Akio Koyama, MD, Kimihiro.

Wound healing around and within saphenous vein bypass grafts

Mesenchymal stem cells attenuate angiotensin II-induced aortic aneurysm growth in apolipoprotein E-deficient mice Ryotaro Hashizume, MD, Aika Yamawaki-Ogata,

Autocrine nerve growth factor is essential for cell survival and viral maturation in HHV-8–infected primary effusion lymphoma cells by Francesca Pica,

Antisense oligonucleotide to proto-oncogene c-myb inhibits the formation of intimal hyperplasia in experimental vein grafts Gregory J. Fulton, MB, FRCSI,

Celiprolol reduces the intimal thickening of autogenous vein grafts via an enhancement of nitric oxide function through an inhibition of superoxide production

Ezetimibe reduces intimal hyperplasia in rabbit jugular vein graft

Fariba Chalajour, MD, Laura A

Reconstruction of pulmonary artery with porcine small intestinal submucosa in a lamb surgical model: Viability and growth potential Lorenzo Boni, MD,

The cyclolignan picropodophyllin attenuates intimal hyperplasia after rat carotid balloon injury by blocking insulin-like growth factor-1 receptor signaling

Volume 117, Issue 5, Pages (November 1999)

Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff Akio Koyama, MD, Kimihiro.

Volume 124, Issue 1, Pages (January 2003)

Decellularization reduces immunogenicity of sheep pulmonary artery vascular patches Eric J. Lehr, MD, PhD, Gina R. Rayat, PhD, Brian Chiu, MD, Thomas.

Blood-derived smooth muscle cells as a target for gene delivery

Volume 114, Issue 2, Pages (February 1998)

Use of Brilliant Blue FCF during vein graft preparation inhibits intimal hyperplasia Michael J. Osgood, MD, Kevin Sexton, MD, Igor Voskresensky, MD, Kyle.

Breast arterial calcification in chronic kidney disease: absence of smooth muscle apoptosis and osteogenic transdifferentiation W. Charles O'Neill, Amy.

Adventitial delivery of platelet-derived endothelial cell growth factor gene prevented intimal hyperplasia of vein graft Mitsuteru Handa, MD, Wei Li,

Increased connexin43 expression in human saphenous veins in culture is associated with intimal hyperplasia Sébastien Déglise, MD, David Martin, MS, Hervé.

Protection of rat spinal cord from ischemia with dextrorphan and cycloheximide: Effects on necrosis and apoptosis Hiroyuki Kato, MD, PhDa c, Georgios.

Vida K. Stark, MS, Thomas F. Warner, MD, John R. Hoch, MD

Vein adaptation to arterialization in an experimental model

Arterial heparan sulfate proteoglycans inhibit vascular smooth muscle cell proliferation and phenotype change in vitro and neointimal formation in vivo

Origin of endothelial cells that line expanded polytetrafluoroethylene vascular grafts sodded with cells from microvascularized fat Stuart K. Williams,

Removal of Stem Cell Factor or Addition of Monoclonal Anti-c-KIT Antibody Induces Apoptosis in Murine Melanocyte Precursors Masaru Ito, Yoko Kawa, Mitsuhiro.

International Journal of Cardiology

Long-term reduction of medial and intimal thickening in porcine saphenous vein grafts with a polyglactin biodegradable external sheath Vikram Vijayan,

Effect of microRNA-145 to prevent vein graft disease in rabbits by regulation of smooth muscle cell phenotype Motoaki Ohnaka, MD, Akira Marui, MD, PhD,

Inhibitory effect of photooxidation on intimal and medial thickening of saphenous vein Jyotirmay Chanda , MD, Ryosei Kuribayashi, MD, Ke-Xiang Liu, MD,

Alterations in wall tension and shear stress modulate tyrosine kinase signaling and wall remodeling in experimental vein grafts Tam T.T. Huynh, MD, Mark.

Contractile smooth muscle cell apoptosis early after saphenous vein grafting Evelio Rodriguez, MD, Erica H Lambert, BS, Michael G Magno, PhD, John D Mannion,

Paclitaxel coating of the luminal surface of hemodialysis grafts with effective suppression of neointimal hyperplasia Insu Baek, MS, Cheng Zhe Bai, PhD,

Vincent L. Rowe, MD, Scott L. Stevens, MD, FACS, Tonya T

Sarpogrelate hydrochloride reduced intimal hyperplasia in experimental rabbit vein graft Akio Kodama, MD, Kimihiro Komori, MD, PhD, Keisuke Hattori, MD,

Insulin-like growth factor 1 prevents neuronal cell death and paraplegia in the rabbit model of spinal cord ischemia Yoshihisa Nakao, MD, Hajime Otani,

Paul H. Schoof, MDa, Adriana C

Allograft heart valves: The role of apoptosis-mediated cell loss

Antisense basic fibroblast growth factor gene transfer reduces early intimal thickening in a rabbit femoral artery balloon injury model David G. Neschis,

Survivin expression is up-regulated in vascular injury and identifies a distinct cellular phenotype Hector F. Simosa, MD, Grace Wang, MD, XinXin Sui,

Adventitial delivery of platelet-derived endothelial cell growth factor gene prevented intimal hyperplasia of vein graft Mitsuteru Handa, MD, Wei Li,

Comparison of cell-type-specific vs transmural aortic gene expression in experimental aneurysms Eiketsu Sho, MD, PhD, Mien Sho, MD, Hiroshi Nanjo, MD,

A self-renewing, tissue-engineered vascular graft for arterial reconstruction Kei Torikai, MD, Hajime Ichikawa, MD, PhD, Koichiro Hirakawa, MS, Goro Matsumiya,

The temporal relationship between the development of vein graft intimal hyperplasia and growth factor gene expression John R. Hoch, MD, Vida K. Stark,

In situ tissue regeneration using a novel tissue-engineered, small-caliber vascular graft without cell seeding Takenori Yokota, MD, Hajime Ichikawa,

Marcel Scheinman, MD, Enrico Ascher, MD, Gabriel S

The effects of ginsenoside Rb1 on endothelial damage and ghrelin expression induced by hyperhomocysteine Zhiwei Xu, PhD, Taohua Lan, MD, Weikang Wu,

Perivascular delivery of losartan with surgical fibrin glue prevents neointimal hyperplasia after arterial injury Michael C Moon, MD, Katerina Molnar,

Pathologic features of cryopreserved aortic allograft implanted in the active infection Akitoshi Yamada, MD, Kenji Okada, MD, Rei Takahashi, MD, Yutaka.

Transduction of peptide analogs of the small heat shock–related protein HSP20 inhibits intimal hyperplasia Deron J Tessier, MD, Padmini Komalavilas,

Influence of vein valves in the development of arteriosclerosis in venoarterial grafts in the rabbit Aurelio Chaux, MDa (by invitation), Xin Min Ruan,

Retaining perivascular tissue of human saphenous vein grafts protects against surgical and distension-induced damage and preserves endothelial nitric.

Yehuda G. Wolf, MD, Lars M. Rasmussen, MD, Yoav Sherman, MD, Warner P

Mostafa N. El-Sanadiki, MB, MS, K. Simon Cross, MB, John J

Murray H. Kown, MDa, Atsushi Yamaguchi, MDa, Christina L

Inhibition of N-cadherin retards smooth muscle cell migration and intimal thickening via induction of apoptosis Cressida A. Lyon, PhD, Evgenia Koutsouki,

Michael E. Landis, MDa, Edward C

Boulos Toursarkissian, MD, David Schwartz, MD, PhD, Paul R

Protein kinase C isoform–dependent myocardial protection by ischemic preconditioning and potassium cardioplegia Kejie Lu, MD*, Hajime Otani, MD, Tadashi.

Expression of the peripheral-type benzodiazepine receptor and apoptosis induction in hepatic stellate cells Richard Fischer, Marcus Schmitt, Johannes.

Immunosuppressive treatment of aortic allografts

External stenting reduces early medial and neointimal thickening in a pig model of arteriovenous bypass grafting Gianni D. Angelini, FRCS, Mohammad Bashar.

Blockade of monocyte chemoattractant protein-1 by adenoviral gene transfer inhibits experimental vein graft neointimal formation Hideki Tatewaki, MD,

An external, oversized, porous polyester stent reduces vein graft neointima formation, cholesterol concentration, and vascular cell adhesion molecule.

Michael L. Marin, MD, Ronald E. Gordon, PhD, Frank J

Locally applied cilostazol suppresses neointimal hyperplasia by inhibiting tenascin-C synthesis and smooth muscle cell proliferation in free artery grafts

Neointimal formation at the sites of anastomosis of the internal thoracic artery grafts after coronary artery bypass grafting in human subjects: An immunohistochemical.

William C. Quist, MD, PhD, Frank W. LoGerfo, MD

Presentation transcript:

Potential role of vacuolar H+–adenosine triphosphatase in neointimal formation in cultured human saphenous vein Hajime Otani, MDa, Hideyasu Ohmiya, MDa, Reiji Hattori, MDa, Hirofumi Fujii, MDa, Hideki Ninomiya, MDa, Masakuni Kido, MDa, Hideki Kawaguchi, MDa, Motohiko Osako, MDa, Hiroji Imamura, MDa, Tetsuo Ohta, MDb, Shoji Ohkuma, PhDc The Journal of Thoracic and Cardiovascular Surgery Volume 119, Issue 5, Pages 998-1007 (May 2000) DOI: 10.1016/S0022-5223(00)70095-X Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions

Fig. 1 Transverse sections of human SV segments stained with elastica van Gieson. A , A freshly isolated vein segment before culture. B , A vein segment after 14 days in culture. C , A vein segment after 14 days in culture in the presence of 10 nmol/L of bafilomycin A1. Arrowheads and arrows point to the internal elastic laminae and the external elastic laminae, respectively. (Original magnification, ×40.) The Journal of Thoracic and Cardiovascular Surgery 2000 119, 998-1007DOI: (10.1016/S0022-5223(00)70095-X) Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions

Fig. 2 Effects of BA1 and the structurally related macrolide antibiotics on thickness of the neointima (A) and the media (B). Human SV segments were cultured for 14 days in the absence or the presence of indicated concentrations of BA1, concanamycin A, FK-506, and erythromycin. The filled bars indicate neointimal thickness; the open bars indicate medial thickness. Preculture (n = 12), vehicle (n = 10), 5 nmol/L BA1 (n = 10), 10 nmol/L BA1 (n = 10), 10 nmol/L concanamycin A (n = 8), FK-506 (n = 10), erythromycin (n = 10). Results are expressed as mean ± SD. ND , Not detected. *P < .001; **P < .0001 vs vehicle. The Journal of Thoracic and Cardiovascular Surgery 2000 119, 998-1007DOI: (10.1016/S0022-5223(00)70095-X) Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions

Fig. 3 Intimal surface structure of human SV segments. Scanning electron micrographs (A -C ; original magnification, ×1000) and immunohistochemical staining for von Willebrand factor (D -F ; original magnification, ×400) are representative of 10 vein segments examined under each of the following conditions: A and D , a freshly isolated vein segment before culture; B and E , a vein segment after 14 days in culture; and C and F , a vein segment after 14 days in culture in the presence of 10 nmol/L of BA1. The Journal of Thoracic and Cardiovascular Surgery 2000 119, 998-1007DOI: (10.1016/S0022-5223(00)70095-X) Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions

Fig. 4 Immunohistochemical staining for α-SMA, desmin, and vimentin. Serial sections obtained from the freshly isolated SV segment were immunostained for α-SMA (A), desmin (B), and vimentin (C). Note that vimentin-positive cells are distributed between the medial smooth muscle cell layers (arrows ) and in the adventitia (arrowhead ). Serial sections obtained from the SV segment after 14 days in culture were immunostained for α-SMA (D), desmin (E), and vimentin (F). Serial sections obtained from the SV segment after 14 days in culture in the presence of 10 nmol/L of BA1 were immunostained for α-SMA (G), desmin (H), and vimentin (I). The arrow points to the vimentin-positive cell. m , Media; a , adventitia; i , intima. (Original magnification, ×100.) The Journal of Thoracic and Cardiovascular Surgery 2000 119, 998-1007DOI: (10.1016/S0022-5223(00)70095-X) Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions

Fig. 5 Immunohistochemical staining for BrdU-labeled cells. The SV segment after 10 days in culture was incubated with 50 μmol/L of BrdU for 48 hours in the absence or presence of 10 nmol/L of BA1. A , Incubation with BrdU in the absence of BA1. B , Incubation with BrdU in the presence of BA1. i , Intima; m , media. (Original magnification, ×400.) The Journal of Thoracic and Cardiovascular Surgery 2000 119, 998-1007DOI: (10.1016/S0022-5223(00)70095-X) Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions

Fig. 6 The apoptotic neointimal cells detected with transmission electron microscopy (A and B ) and with TUNEL with the use of light microscopy (C) or confocal laser microscopy (D). The neointimal cell of the vein segment treated with 50 nmol/L of BA1 for 24 hours after 10 days in culture shows chromatin condensation at the periphery of the nucleus associated with cellular shrinkage and formation of vesicles in the presence of intact plasma membrane (A). The neointimal cell shows nuclear fragmentation and disintegration of intracellular organelles after 48 hours of treatment with BA1 (B). (Original magnifications, A and B, ×8000.) C , The vein segment treated with 50 nmol/L of BA1 for 48 hours after 10 days in culture shows that most of the neointimal cells are positive for TUNEL, although few medial cells are positive for these indices of apoptosis. i , Intima; m , media. (Original magnification, ×200.) D , A serial section of the vein segment was doubly immunostained for TUNEL and desmin with fluorescein isothiocyanate–conjugated and tetrarhodamine isothiocyanate–conjugated secondary antibodies, respectively. The immunofluorescence staining was visualized with confocal laser microscopy. Note that the neointimal cells with positive immunofluorescence for TUNEL (arrowhead ) are negative for desmin, although medial cells with negative immunofluorescence for TUNEL are positive for desmin (arrow ). Bar , 10 μm. The Journal of Thoracic and Cardiovascular Surgery 2000 119, 998-1007DOI: (10.1016/S0022-5223(00)70095-X) Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions

Fig. 7 The dose response effect of BA1 on apoptosis. The SV segments after 10 days in culture were treated with 50 nmol/L of BA1 for 48 hours. Double immunofluorescence confocal laser microscopy for identifying TUNEL-positive cells with or without expression of desmin was performed as described. The percentage of TUNEL-positive cells was calculated by dividing the number of TUNEL-positive cells with positive immunofluorescence staining for desmin in the media or the number of those cells with negative immunofluorescence staining for desmin in the neointima by the total number of cells with a respective immunoreactivity pattern for desmin in each layer. Open bars indicate medial cells expressing desmin; filled bars indicate neointimal cells not expressing desmin. Results are expressed as mean ± SD of 7 experiments. *P < .05; **P < .0001, compared with the vehicle (0.1% dimethylsulfoxide) group; †P < .05; ††P < .001, compared with the desmin-positive medial cell group. The Journal of Thoracic and Cardiovascular Surgery 2000 119, 998-1007DOI: (10.1016/S0022-5223(00)70095-X) Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions