A comparative study of two extraction methods routinely used for DNA recovery from simulated post coital samples  T. Mudariki, H. Pallikarana-Tirumala,

Slides:



Advertisements
Similar presentations
The most efficient STR loci in forensic genetics in population of central Poland R. Jacewicz, M. Jedrzejczyk, J. Berent Forensic Science International:
Advertisements

A comparison of AmpFlSTR Identifiler™ Kit versus AmpFlSTR Identifiler Plus™ Kit in challenging bone samples by using normal and increased PCR cycle number 
Quantifiler® Trio DNA validation and usefulness in casework samples
Collection protocols for the recovery of biological samples
A comparison of AmpFlSTR Identifiler™ Kit versus AmpFlSTR Identifiler Plus™ Kit in challenging bone samples by using normal and increased PCR cycle number 
Exploring the relative DNA contribution of first and second object’s users on mock touch DNA mixtures  F. Oldoni, V. Castella, D. Hall  Forensic Science.
A. Verzeletti, V. Cortellini, S. Cisana, S. Pretto, N. Cerri, F
Genetic profiling from challenging samples: Direct PCR of touch DNA
S. Alimat, S. Hadi, W. Goodwin 
Quantifiler® Trio DNA validation and usefulness in casework samples
S. Severini, M. Lancia, S. Massetti, A. Coletti, L. Carlini, E
R. Alghafri, W. Goodwin, S. Hadi 
M. Scherer, B. Alsdorf, M. Breitbach, K. Matthaei, A. Schnur, N
Stefania Turrina, Stefano Caratti, Domenico De Leo 
A priori probabilities in Y23 mixture analysis: Non-contributor experiments using simulated powerplex® Y23 Y-STR mixtures  D. Moore, T. Clayton, J. Thomson 
Performance of the RapidHIT™200
A. Barbaro, P. Cormaci, G. Falcone 
Successful STR and SNP typing of FTA Card samples with low amounts of DNA after DNA extraction using a Qiagen BioRobot® EZ1 Workstation  Eszter Rockenbauer,
Wei Deng, Meili Lv, Li Wang, Peng Bai, Weibo Liang, Lin Zhang 
Probabilistic modelling for DNA mixture analysis
C. Davies, J. Thomson, F. Kennedy 
The effect of increased cycle numbers using the Quantifiler® Duo DNA Quantification Kit (AB) on the detection of minute amounts of male DNA in mixtures.
The effects of different adhesive tapes and of Hemastix test strips on DNA recovery using magnetic silica beads  Stefan Kutranov, Holly Cullis  Forensic.
DNA typing of trace DNA recovered from different areas of sandals found at a homicide crime scene investigation: A comparative study  Samuel T.G. Ferreira,
Establishment of Italian national DNA database and the central laboratory: Some aspects  R. Biondo, F. De Stefano  Forensic Science International: Genetics.
U. Ricci, I. Carboni, S. Iozzi, A. Gozzini, F. Torricelli 
Seung-Bum Hong, Yangseop Kim, Jung-Hyun Park 
R. Alghafri, S. Alhammadi, K. Amiri 
Population data for SE33 locus in United Arab Emirates Arab population
Christina Valgren, Sara Wester, Oskar Hansson 
S. Iyavoo, A. Wolejko, D. Furmanczyk, R. Graham, R. Myers, T. Haizel 
A rape case with multiple unknown perpetrators
Automated extraction of DNA from clothing
The development of visual and chemical methods for predicting the likelihood of obtaining a DNA profile from degraded bone samples  M. Al-enizi, S. Hadi,
Combining DNA evidence for greater match information
J. Hedman, L. Albinsson, L. Norén, R. Ansell 
A. Verzeletti, V. Cortellini, S. Cisana, S. Pretto, N. Cerri, F
Body fluid mixtures: Resolution using forensic microRNA analysis
Timothy J. Verdon, Kaye N. Ballantyne, R. John Mitchell, Roland A. H
Is the stamp son of the acoustic prothesis?
Efficient DNA extraction from hair shafts
Denilce R. Sumita, Martin R. Whittle 
Evaluating DNA profiles using peak heights, allowing for dropin, dropout and stutters  Roberto Puch-Solis  Forensic Science International: Genetics Supplement.
Joanne L. Simons, Amelia Gamblin, Laura Arnold, Sue C. Vintiner 
Multiplex DNA amplification and barcoding in a single reaction for 454 Roche sequencing: A comprehensive study on the control region of the mitochondrial.
Validation study of endogenous reference genes for normalization of quantitative real time PCR data in post mortem skin tissue  I. Gomes, K. Becker, M.A.
A review of low template STR analysis in casework using the DNA SenCE post-PCR purification technique  T. Gross, J. Thomson, S. Kutranov  Forensic Science.
The study of single nucleotide polymorphisms (SNPs) in Arab populations—A tool for the analysis of degraded DNA  S.H. Sanqoor, S. Hadi, W. Goodwin  Forensic.
A comparison of AmpFlSTR Identifiler™ Kit versus AmpFlSTR Identifiler Plus™ Kit in challenging bone samples by using normal and increased PCR cycle number 
Distribution of Y chromosome haplogroup Q in Greenlanders
Introducing a latent variable approach for finding populations in a forensic DNA database  Maarten Kruijver  Forensic Science International: Genetics.
Determination of siblings: A special case report from Halle
C.J. Bruijning-van Dongen, K. Slooten, W. Burgers, W. Wiegerinck 
On the exact distribution of the numbers of alleles in DNA mixtures
Direct STR typing from bullet casings
Testing the behavior of GlobalFiler® PCR amplification kit with degraded and/or inhibited biological samples  V. Bogas, M. Carvalho, F. Corte-Real, M.J.
M. Hara, T. Masuda, A. Takada, T. Miyazaki, H. Suzuki, A. Kido, K
Semen detection: A retrospective overview from 2010
Application of less primer method to commercial kits
DNA persistance in soft tissues exposed to extreme environments
S. Iyavoo, S. Hadi, W. Goodwin 
Simulated approach to estimate the number and combination of known/unknown contributors in mixed DNA samples using 15 short tandem repeat loci  Sho Manabe,
Effects of the most common methods for the enhancement of latent fingerprints on DNA extraction from forensic samples  S. Gino, M. Omedei  Forensic Science.
M. Lurdes Pontes, M. Fátima Pinheiro 
Evaluation of the Differex™ System
Improved performance for forensic casework: Extraction and isolation updates for the Maxwell® 16 instrument  M. Lindner, P.V. Mandrekar, J. Bessetti,
Development of a 24-plex Y chromosomal STR loci typing system
First application of the Investigator DIPplex indels typing kit for the analysis of ancient DNA samples  C. Hollard, F. Mendisco, C. Keyser, E. Crubézy,
Discrepancies between forensic DNA databases
A.M. Ölçen, G. Filoğlu, H. Altunçul, S. Erdem, Ö. Bülbül 
Presentation transcript:

A comparative study of two extraction methods routinely used for DNA recovery from simulated post coital samples  T. Mudariki, H. Pallikarana-Tirumala, L. Ives, S. Hadi, W. Goodwin  Forensic Science International: Genetics Supplement Series  Volume 4, Issue 1, Pages e194-e195 (January 2013) DOI: 10.1016/j.fsigss.2013.10.100 Copyright © 2013 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Comparison of DNA concentrations from samples extracted using Differex™ and QIAamp® measured using Nanodrop 2000. Very similar results were obtained when using Quantifiler®. Forensic Science International: Genetics Supplement Series 2013 4, e194-e195DOI: (10.1016/j.fsigss.2013.10.100) Copyright © 2013 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 Percentage of profiles falling into different classifications. Samples extracted with QIAamp® and Differex™ and profiled using AmpFℓSTR® SGM Plus® and PowerPlex® ESI 17. Forensic Science International: Genetics Supplement Series 2013 4, e194-e195DOI: (10.1016/j.fsigss.2013.10.100) Copyright © 2013 Elsevier Ireland Ltd Terms and Conditions