Sperm DNA fragmentation induced by cryopreservation: new insights and effect of a natural extract from Opuntia ficus-indica Mehrdad Meamar, Ph.D., Nassira.

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Presentation transcript:

Sperm DNA fragmentation induced by cryopreservation: new insights and effect of a natural extract from Opuntia ficus-indica Mehrdad Meamar, Ph.D., Nassira Zribi, Ph.D., Marta Cambi, Lara Tamburrino, Ph.D., Sara Marchiani, Ph.D., Erminio Filimberti, Ph.D., Maria Grazia Fino, Annibale Biggeri, M.D., Yves Menezo, Ph.D., Gianni Forti, M.D., Elisabetta Baldi, Ph.D., Monica Muratori, Ph.D. Fertility and Sterility Volume 98, Issue 2, Pages 326-333 (August 2012) DOI: 10.1016/j.fertnstert.2012.05.001 Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Sperm DNA fragmentation (SDF) after cryopreservation in the presence or absence of Opuntia ficus-indica (OFI) extracts and resveratrol. (A) Individual SDF values in the PIbr population before and after cryopreservation in basal conditions (left: control, n = 21) and in the presence of OFI extracts (center: n = 15) and resveratrol (right: n = 15). (B) Scatterplot and linear regression analysis of values before and after cryopreservation in basal conditions with 95% confidence interval. Fertility and Sterility 2012 98, 326-333DOI: (10.1016/j.fertnstert.2012.05.001) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Scatterplots and linear regression between sperm DNA fragmentation (PIdim population) and the sperm motility parameters of amplitude of lateral head displacement (ALH: R = −0.55, P=.03), straightness (STR: R = 0.59, P=.02), beat-cross frequency (BCF: R = 0.66, P=.006), and linearity (LIN: R = 0.55, P=.03), as evaluated by CASA. Fertility and Sterility 2012 98, 326-333DOI: (10.1016/j.fertnstert.2012.05.001) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions