Round spermatid injection

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Presentation transcript:

Round spermatid injection Sherman J Silber, M.D., Larry Johnson, Ph.D., Greta Verheyen, M.D., Andre Van Steirteghem, Ph.D. Fertility and Sterility Volume 73, Issue 5, Pages 897-900 (May 2000) DOI: 10.1016/S0015-0282(00)00488-X

Figure 1 A tubule with normal spermatogenesis in a testicular specimen that is otherwise Sertoli cell only. Each Sertoli cell nucleus at the base of the tubule is the same “cell” as the “round cell” in the wet testicular sperm extraction preparation (stain, hematoxylin and eosin; original magnification, ×400). Silber. Round spermatid injection. Fertil Steril 2000. Fertility and Sterility 2000 73, 897-900DOI: (10.1016/S0015-0282(00)00488-X)

Figure 2 Sertoli cell only, viewed using Hoffman optics (wet prep.). Arrows indicate Sertoli cell nuclei (original magnification, ×400). Silber. Round spermatid injection. Fertil Steril 2000. Fertility and Sterility 2000 73, 897-900DOI: (10.1016/S0015-0282(00)00488-X)

Figure 3 Stages of spermiogenesis: stage 1 (1a, b, and c), stage 2 (2a and b), and stage 3 (elongated spermatid) (3) (11). Silber. Round spermatid injection. Fertil Steril 2000. Fertility and Sterility 2000 73, 897-900DOI: (10.1016/S0015-0282(00)00488-X)

Figure 4 Dispersed, live, human testicular cells as observed by Nomarski optics. Sertoli cell nuclei (SC) are nonspherical, although some appear spherical. Sertoli cell cytoplasm is granular in appearance (open arrow). Germ cells with spherical nuclei include primary spermatocytes (PS) and round spermatids (RS). Testicular sperm (TS) are detached from other cells. Occasionally, degenerating germ cells (primary spermatocytes; DP) are observed in culture. Bar, 10 μm. (From Johnson L, Texas A & M University.) Silber. Round spermatid injection. Fertil Steril 2000. Fertility and Sterility 2000 73, 897-900DOI: (10.1016/S0015-0282(00)00488-X)