The effect of SIRT1 on differentiation of MSCs in vitro. A–D

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The effect of SIRT1 on differentiation of MSCs in vitro. A–D The effect of SIRT1 on differentiation of MSCs in vitro.A–D.Single cell MSC proliferation and differentiation. The effect of SIRT1 on differentiation of MSCs in vitro.A–D.Single cell MSC proliferation and differentiation. Differentiation to osteoblasts on the left and to adipocytes on the right. (A) Cell number per well (n = 12 wells per group), (B) FACS analysis of apoptotic annexin V positive cells per well (n = 6 wells per group), (C) FACS analysis of ALP+ osteoblasts per well (*p = 0.04, t‐test, n = 12 wells per group) on the left and FACS analysis of FABP4+ adipocytes per well (n = 12 wells per group) on the right, (D) FACS analysis of STRO‐1+ mesenchymal progenitor cells per well (*p = 0.03 on the left and p = 0.05 on the right, t‐test, n = 6 wells per group); bars, SD.E.FACS analysis of percentage of MSCs in the bone marrow of old (left, *p = 0.01, t‐test, n = 6 per group) and young (right) mice.F.Alkaline phosphatase (ALP) staining of MSCs (isolated by the plating method) differentiated towards osteoblasts. BFU bone forming units, numbers are mean ± SD; *p = 0.001, t‐test, n = 12 wells per group.G.Alcian blue staining of MSCs differentiated towards adipocytes. CFU/alcian blue+, colony forming units/Alcian blue positive, numbers are mean ± SD; *p = 0.03, t‐test, n = 6 wells per group.H.LipidTOX staining of MSCs differentiated towards adipocytes, *p = 0.05, t‐test, n = 6 wells per group.I.ALP staining of MSCs (isolated by the plating method) after fl/fl and MSCKO bone marrow transplantation (BMT) and differentiated towards osteoblasts. Numbers are mean ± SD; *p = 0.01, t‐test, n = 12 wells per group. Petra Simic et al. EMBO Mol Med. 2013;5:430-440 © as stated in the article, figure or figure legend