Lymphocyte lineage trees produced by long-term live cell microscopy.

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Lymphocyte lineage trees produced by long-term live cell microscopy. Lymphocyte lineage trees produced by long-term live cell microscopy. (A) Schematic of the live-cell time-lapse microscopy pipeline. See Movie S1 for annotated example of single-cell lineage tree construction. (B) Representative lineage tree for CpG-stimulated splenic mature B cells. (C) Stacked area plot of total cell count of the tracked cells with the contribution of each generation marked with similar color used in Fig. 1C. (D) Representative lineage tree for CpG-stimulated Follicular B cells. (E) Stacked area plot of total cell count of the tracked cells with the contribution of each generation marked with similar color used in Fig. 1C. (F) Representative lineage tree for CpG-stimulated splenic marginal zone B cells. (G) Stacked area plot of total cell count of the tracked cells with the contribution of each generation marked with similar color used in Fig. 1C. TIFF, tagged image file format. Simon Mitchell et al. PNAS doi:10.1073/pnas.1715639115 ©2018 by National Academy of Sciences