Genotoxicity assessment of mouse oocytes by comet assay before vitrification and after warming with three vitrification protocols  Anais Berthelot-Ricou, M.D., Jeanne Perrin, M.D., Ph.D., Carole di Giorgio, Ph.D., Michel de Meo, Ph.D., Alain Botta, M.D., Pharm.D., Ph.D., Blandine Courbiere, M.D., Ph.D.  Fertility and Sterility  Volume 100, Issue 3, Pages 882-888 (September 2013) DOI: 10.1016/j.fertnstert.2013.05.025 Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Alkaline comet assay on mouse oocyte after exposure to equilibration and vitrification solutions according to protocols 1, 2, and 3. Olive tail moment (OTM): product of the percentage of the DNA in the tail and the distance between the center of the head and the tail. Neg Cont: negative control oocyte group in M2 medium at ambient temperature just after hyaluronidase exposure. Pos Cont: positive control oocyte group incubated in a 250-μmol/L H2O2 solution for 5 minutes at 4°C in the dark. P1, P2, and P3: oocyte groups exposed to equilibration and vitrification solutions according to protocols 1, 2, and 3. Fertility and Sterility 2013 100, 882-888DOI: (10.1016/j.fertnstert.2013.05.025) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Alkaline comet assay on mouse oocyte after vitrification, liquid nitrogen storage, and thawing according to protocols 1, 2, and 3. Olive tail moment (OTM): product of the percentage of the DNA in the tail and the distance between the center of the head and the tail. Neg Cont: negative control oocyte group incubated in M16 medium at 37°C and 5% CO2 for 3 hours just after hyaluronidase exposure. Pos Cont: positive control oocyte group incubated in a 250-μM H2O2 solution for 5 minutes at 4°C in the dark. P1, P2, and P3: oocytes vitrified with protocols 1, 2 and 3 and rehydrated with the corresponding thawing kits according to each manufacturer’s instructions. Fertility and Sterility 2013 100, 882-888DOI: (10.1016/j.fertnstert.2013.05.025) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions