Song playbacks induce pS6 in the P30 male auditory forebrain.

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Song playbacks induce pS6 in the P30 male auditory forebrain. Song playbacks induce pS6 in the P30 male auditory forebrain. (A) Fold change in Song/Sil pS6+/S6+ cell density in the CMM (Left) and NCM (Right). Bars (white, males; gray, females) represent the experimental group mean ± SEM. Open circles (○) denote individual birds. *P < 0.005, compared with the sex-matched P23 group; #P < 0.005, compared with the sex-matched P30 group; $P < 0.05 between age-matched sexes (n = 4 birds per Sex and Age/Rearing condition). (B) Baseline (Silence) pS6+/S6+ cell densities in the CMM (Left) and NCM (Right). (C) Representative bright-field images of pS6+ and total S6+ cells in P23, P30, and P30i male auditory forebrain; below each is a higher magnification image of the boxed Inset. The dashed oval indicates the position of Field L. Brightness and contrast were adjusted for figure clarity, but not for analysis. (Scale bars: auditory forebrain, 500 μm; Insets, 250 μm.)‏ Somayeh Ahmadiantehrani, and Sarah E. London PNAS 2017;114:35:9463-9468 ©2017 by National Academy of Sciences