Single Base Instability Is Promoted in Vulvar Lichen Sclerosus Ronald A. Tapp, Jingtao Feng, J. Wesley Jones, J. Andrew Carlson, Vincent L. Wilson  Journal of Investigative Dermatology  Volume 127, Issue 11, Pages 2563-2576 (November 2007) DOI: 10.1038/sj.jid.5700889 Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 TP53 and Ki-67 labeling in normal and LS-affected genital skin sections. Top three panels illustrate results for histologically normal genital skin, which showed a weak, dispersed pattern of TP53 nuclear immunoreactivity (top middle panel). In contrast, most samples of LS showed circumscribed patches of strong, dispersed, TP53 nuclear labeling (middle panel), or well demarcated compact patches of strong nuclear labeling (bottom middle panel), the latter of which are termed epidermal TP53 clones (Ling et al., 2001). Ki-67 labeling, which measures the growth fraction (number or cells in G1, S, G2, and M of the cell-cycle), was similar in all cases (right panels), indicating that aberrant TP53 protein expression did not impact on keratinocyte proliferation. This latter finding of lack of correlation between TP53 and Ki-67 labeling has also been described for sun-damaged skin harboring epidermal TP53 clones (Tabata et al., 1999). (All original magnifications at × 200. Left – hematoxylin and eosin stained section; middle panel – TP53 immunolabeling; right- Ki-67 immunolabeling). Journal of Investigative Dermatology 2007 127, 2563-2576DOI: (10.1038/sj.jid.5700889) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions