UniFrac analysis at high time resolution.

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UniFrac analysis at high time resolution. UniFrac analysis at high time resolution. These data are from six healthy volunteers (labeled HTR1–HTR6) in the age range of 17–30 y separate from the main text study, sampled at days −5, −3, 0, 1, 4, 7, 9, and 11 relative to 2012 seasonal trivalent inactivated influenza vaccine administration. BCR libraries were prepared and sequenced using a previously published protocol (11) very similar to the protocol used for the main text cohort. Participants HTR1, HTR2, HTR3, HTR4, HTR5, and HTR6 were aged 18, 28, 25, 24, 19, and 28 y, respectively, and were female, male, male, male, female, and male, respectively. (A) Heat map of UniFrac values between participants and time points, clustered using complete linkage. (B) Within-participant longitudinal UniFrac distances as a function of time interval. Day 7 marks peak vaccine response. Five of 6 participants showed an increase in UniFrac from the smallest time interval to the largest. Charles F. A. de Bourcy et al. PNAS 2017;114:5:1105-1110 ©2017 by National Academy of Sciences