Activation of Gα subunit chimeras with noncognate C termini.

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Activation of Gα subunit chimeras with noncognate C termini. Activation of Gα subunit chimeras with noncognate C termini. (A) Gsi, Gsq, and Gs12 chimeras fully restored cAMP accumulation in response to D1R stimulation (10 μM SKF 81297) in cells lacking endogenous Gαs family subunits (n = 4). (B) Gqs, Gqi, and Gq12 chimeras fully restored calcium release in response to M3R stimulation (100 μM acetylcholine) in cells lacking endogenous Gαq family subunits (n = 6). (C) Gqi but not Gqs and Gq12 chimeras fully restored calcium release in response to H1R stimulation (100 μM histamine) in cells lacking endogenous Gαq subunits (n = 4). Traces are averages of several experiments, and data points represent independent experiments. Pertussis toxin S1 subunit (PTX) was coexpressed as indicated. Najeah Okashah et al. PNAS doi:10.1073/pnas.1905993116 ©2019 by National Academy of Sciences