Intermittent hydrostatic pressure inhibits matrix metalloproteinase and pro-inflammatory mediator release from human osteoarthritic chondrocytes in vitro

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Intermittent hydrostatic pressure inhibits matrix metalloproteinase and pro-inflammatory mediator release from human osteoarthritic chondrocytes in vitro Michael C.D. Trindade, M.S., Jun-ichi Shida, M.D., Ph.D., Takashi Ikenoue, M.D., Ph.D., Mel S. Lee, M.D., Eric Y. Lin, M.D., Burt Yaszay, M.D., Scott Yerby, Ph.D., Stuart B. Goodman, M.D., Ph.D., David J. Schurman, M.D., R.Lane Smith, Ph.D. Osteoarthritis and Cartilage Volume 12, Issue 9, Pages 729-735 (September 2004) DOI: 10.1016/j.joca.2004.05.008

Fig. 1 Schematic representation of the system for application of intermittent hydrostatic pressure. A frequency of 1Hz is maintained by a computer controller interfaced to an MTS 858 servo-hydraulic loading frame (MTS Systems Corporation, Eden Prairie, MN). Temperature of the loaded and unloaded chondrocyte cultures was controlled at 37°C in a circulating water bath. Osteoarthritis and Cartilage 2004 12, 729-735DOI: (10.1016/j.joca.2004.05.008)

Fig. 2 Effects of intermittent hydrostatic pressure (IHP) on human osteoarthritic chondrocyte matrix metalloproteinase-2 release into culture medium. Each data plot represents the mean results for six separate cell preparations in which each experimental condition was tested in triplicate. * Denotes P<0.05 vs. control cultures (CTL) maintained in the absence of IHP for the same time period tested. Osteoarthritis and Cartilage 2004 12, 729-735DOI: (10.1016/j.joca.2004.05.008)

Fig. 3 Zymographic analysis of matrix metalloproteinase release into culture medium. Lanes 1–3 represent samples for six (6C), twelve (12C) and twenty-four (24C) hour control cultures maintained in the absence of IHP. Lanes 4–6 represent cultures maintained in the presence of IHP for six (6I), twelve (12I) and twenty-four (24I) hours. MMP-2 (72kDa gelatinase) and MMP-9 (92kDa gelatinase) migrate in denaturing gel systems at 68/62kDa and 84/82kDa, respectively, when preactivated with APMA. Osteoarthritis and Cartilage 2004 12, 729-735DOI: (10.1016/j.joca.2004.05.008)

Fig. 4 Effects of intermittent hydrostatic pressure (IHP) on human osteoarthritic chondrocyte TIMP-1 release into culture medium. Each data plot represents the mean results for six separate cell preparations in which each experimental condition was tested in triplicate. Osteoarthritis and Cartilage 2004 12, 729-735DOI: (10.1016/j.joca.2004.05.008)

Fig. 5 Effects of intermittent hydrostatic pressure (IHP) on human osteoarthritic chondrocyte interleukin-6 release into culture medium. Each data plot represents the mean results for six separate cell preparations in which each experimental condition was tested in triplicate. * Denotes P<0.05 vs. control cultures (CTL) maintained in the absence of IHP for the same time period tested. Osteoarthritis and Cartilage 2004 12, 729-735DOI: (10.1016/j.joca.2004.05.008)

Fig. 6 Effects of intermittent hydrostatic pressure (IHP) on human osteoarthritic chondrocyte monocyte chemoattractant protein-1 release into culture medium. Each data plot represents the mean results for six separate cell preparations in which each experimental condition was tested in triplicate. * Denotes P<0.05 vs. control cultures (CTL) maintained in the absence of IHP for the same time period tested. Osteoarthritis and Cartilage 2004 12, 729-735DOI: (10.1016/j.joca.2004.05.008)