Purity of Lnp and its derivatives.

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Purity of Lnp and its derivatives. Purity of Lnp and its derivatives. (A) SEC of His10-tagged Xenopus Lnp. Shown is the absorbance at 280 nm. (B) As in (A), but for human Lnp. The second peak originates from the detergent Triton X-100 used for the purification of the protein. (C) Purified wild-type and mutant Lnp proteins were analyzed by SDS-PAGE and Coomassie blue staining. Lines in between lanes indicate slices that were merged from different gels. The proteins shown all contain the two TM segments. For purity of the cytosolic fragments, see Wang et al (16). Songyu Wang et al. LSA 2018;1:e201700014 © 2018 Wang et al