Treatment of WiT49 cells with NVP-AEW541 induces gene expression changes associated with cell cycle arrest and PI3K/MAPK down-regulation, and sensitizes.

Slides:

Advertisements

Similar presentations

Comparison (θ) of the mean dinucleotide variability distribution for (A) genes with MAE vs. Comparison (θ) of the mean dinucleotide variability distribution.

Advertisements

Comparison (R) of the mean observed to expected dinucleotide distribution for (A) genes with MAE vs. Comparison (R) of the mean observed to expected dinucleotide.

Balancing selection characterizes both MAE genes and cell type-specific genes (expressed in either nsc or asl but not in both). Balancing selection characterizes.

(A) Heat map showing expression changes during in vitro differentiation of nscs to astrocytes. (A) Heat map showing expression changes during in vitro.

Principal component analysis of the GO category composition of all genes in each genome/transcriptome and WGD paralogs. Principal component analysis of.

Immunity-related genes and enhancers are enriched for eQTLs.

High-throughput drug screen and validation to nominate patient-specific therapeutic options. High-throughput drug screen and validation to nominate patient-specific.

Gene-expression changes associated with stunting.

H3K4me3 pattern association with functionally relevant genes and changes over time. H3K4me3 pattern association with functionally relevant genes and changes.

Fig. 4. BET bromodomain inhibition suppresses transactivation of shared transcriptional networks across HF models. BET bromodomain inhibition suppresses.

P53 Mediates Vast Gene Expression Changes That Contribute to Poor Chemotherapeutic Response in a Mouse Model of Breast Cancer Crystal Tonnessen-Murray,

Transcriptional response to mating in whole A. gambiae females.

Host transcriptional profiling distinguishes patients with acute LRTI (LRTI+C+M) from those with noninfectious acute respiratory illness (no-LRTI). Host.

Genome-wide CRISPR screen correctly identifies known regulators of proximal TCR signaling. Genome-wide CRISPR screen correctly identifies known regulators.

RNA-seq analysis of iWAT and eWAT.

Stem/progenitor-specific TF profiling identifies ligands of major pathways. Stem/progenitor-specific TF profiling identifies ligands of major pathways.

Combined LRTI prediction metric integrating pathogen detection and host gene expression. Combined LRTI prediction metric integrating pathogen detection.

HIS-24 regulates expression of infection-inducible genes.

Volume 11, Issue 2, Pages (August 2012)

Volume 7, Issue 4, Pages (April 2005)

Comparison of N. gonorrhoeae gene expression during infection in men and women in vivo. Comparison of N. gonorrhoeae gene expression during infection in.

LincRNAs expressed in specific subpopulations of mESCs and NPCs.

Prescribed forcings. Prescribed forcings. (Top) Volcanic forcing is indicated as global visible optical depth. (Middle) Solar forcing is obtained by scaling.

Patient neural progenitors show a neurodevelopmental phenotype.

Chromatin binding sites shared by pCREB1 and ERα are predominantly cAMP induced. Chromatin binding sites shared by pCREB1 and ERα are predominantly cAMP.

Anastasia Baryshnikova Cell Systems

BET and MEK inhibition synergistically impair cell cycle regulation and activate apoptotic signaling BET and MEK inhibition synergistically impair cell.

JNK signature in breast cancer cells is linked to ECM, stem cell, and wound healing gene networks and is enriched in basal‐like breast cancer JNK signature.

High-throughput combination drug screening to nominate potent drug combinations. High-throughput combination drug screening to nominate potent drug combinations.

Volume 49, Issue 2, Pages (January 2013)

Ivan C. Gerling et al. JCHF 2013;1:

Functional enrichment of differentially expressed genes.

The BRD4 bromodomain is critical for expression of SASP genes.

Boosting Signal-to-Noise in Complex Biology: Prior Knowledge Is Power

Chromosome 8 cDNA microarray gene expression profile of the amplified regions of 8p11–12 in SUM-44, -52, and -225 cells versus MCF10A HME control cells.

Volume 22, Issue 3, Pages (January 2018)

Significant differences in translational efficiencies of DNA damage repair pathway genes between patient clusters. Significant differences in translational.

Hironori Hara et al. BTS 2018;3:

Existence of a nuclear NFATc1–STAT3 complex in pancreatic cancer.

AZA treatment induces a distinct gene-expression pattern in stromal cells. AZA treatment induces a distinct gene-expression pattern in stromal cells. (A-C)

Treatment with NVP-AEW541 shows no efficacy in an s. c

Intestinal differentiation is coupled with global rewiring of gene expression Intestinal differentiation is coupled with global rewiring of gene expression.

Clustering of residues for which PREs unaccounted for by the main state were measured. Clustering of residues for which PREs unaccounted for by the main.

Treatment with DM-α-KG rescues gene expression in the context of chronic mtDNA depletion. Treatment with DM-α-KG rescues gene expression in the context.

Tissue-specific transcriptome responses to fasting.

The up-regulation of Galnt3 by Pi requires activation of the ERK pathway and induction of Egr1 and Etv5. The up-regulation of Galnt3 by Pi requires activation.

Mimic treatment induces eWAT inflammation and hepatic steatosis.

RNA expression data. RNA expression data. Heat maps comparing the normalized log2 of the ratios of Fur−, F90, and F1 signals on the cl20 control signal.

Ingenuity pathway analysis of the genes enriched in both 96-h-induced MMs and human ccRCC. Ingenuity pathway analysis of the genes enriched in both 96-h-induced.

Comparison ofMyc-induced zebrafish liver tumors with different stages of human HCC and seven mouse HCC models. Comparison ofMyc-induced zebrafish liver.

Statistical chart of significantly differentially expressed genes

Genetic maintenance of histone acetylation prevents gene expression changes in the promoters of genes responding to acute mtDNA depletion. Genetic maintenance.

Reanalysis of an existing experiment using ADAGE

PMPs survive STZ cytotoxicity and produce larger colonies with a higher yield of insulin+ progeny. PMPs survive STZ cytotoxicity and produce larger colonies.

Extended analysis of differential expression datasets.

Enrichment of KEGG pathways in microbial genes in different samples.

Transcriptomic changes in strains PAK and PAKpmrB6 in response to polymyxin B (PMB). Transcriptomic changes in strains PAK and PAKpmrB6 in response to.

Volume 9, Issue 5, Pages (November 2011)

Fig. 5 n-HA regulates gene expressions related to tumor suppression, calcium homeostasis, and immune response. n-HA regulates gene expressions related.

Transcriptional and genomic targets of EN1 in TNBC cells.

CREBBP loss-of-function results in gene expression repression signature. CREBBP loss-of-function results in gene expression repression signature. A–D,

Generation of an HPV–human PPI map.

Fig. 3 DUB proteins act at different levels of type I IFN pathways.

Real-time PCR with SYBR Green Master Mix was used to quantify the expression levels of 84 genes ontologically related and regulated by TGF-β /BMP signaling.

Distinct subtypes of CAFs are detected in human PDAC

Panobinostat induces reversible perturbations in PBMC gene expression patterns. Panobinostat induces reversible perturbations in PBMC gene expression patterns.

Driver pathways and key genes in OSCC

Identification and characterization of activated integrin pathway module by signal transduction representation (A) and unsupervised hierarchical clustering.

Transcriptome profiling of PD-L1 antibody–treated macrophages showed inflammatory phenotype, increased survival and proliferation, and decreased apoptosis.

Characteristic gene expression patterns distinguish LCH cells from other immune cells present in LCH lesions. Characteristic gene expression patterns distinguish.

Presentation transcript:

Treatment of WiT49 cells with NVP-AEW541 induces gene expression changes associated with cell cycle arrest and PI3K/MAPK down-regulation, and sensitizes the cells to chemotherapy. Treatment of WiT49 cells with NVP-AEW541 induces gene expression changes associated with cell cycle arrest and PI3K/MAPK down-regulation, and sensitizes the cells to chemotherapy. (A) Heat map demonstrates expression of genes associated with cell cycle progression in WiT49 cells treated with 5× GI50 of NVP-AEW541 for 1, 6, and 24 h. Genes are colored according to global, not relative, expression values (blue, down-regulated; red, up-regulated). Top-ranking gene set enrichment analysis scores for GNF2_CCNA2 [enrichment score = −0.89, P < 0.00001, false discovery rate (FDR) q < 0.00001] GNF2_CKS2 (enrichment score= −0.88, P < 0.00001, FDR q < 0.00001), and GNF2_CENPF (enrichment score = −0.85, P < 0.00001, FDR q < 0.00001) highlights coordinate down-regulation of genes in the expression neighborhood of these cell cycle control genes. (B) Top-ranking canonical pathways identified by Ingenuity Pathway Analysis after treatment of WiT49 cells with NVP-AEW541 were those associated with PI3K/AKT (P < 0.0001) and ERK/MAPK signaling (P < 0.0001). Pathway components are colored by their global gene expression levels (green, down-regulated; red, up-regulated). (C) Median effects analysis of combining NVP-AEW541 with chemotherapeutic agents in WiT49 cells. Highly synergistic interactions are seen for doxorubicin (combination index = 0.43) and topotecan (combination index = 0.39). Aleksandra Bielen et al. PNAS 2012;109:20:E1267-E1276 ©2012 by National Academy of Sciences