C. Abril, E.M. Vilei, I. Brodard, A. Burnens, J. Frey, R. Miserez

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Discovery of insertion element ISCfe1: a new tool for Campylobacter fetus subspecies differentiation C. Abril, E.M. Vilei, I. Brodard, A. Burnens, J. Frey, R. Miserez Clinical Microbiology and Infection Volume 13, Issue 10, Pages 993-1000 (October 2007) DOI: 10.1111/j.1469-0691.2007.01787.x Copyright © 2007 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Genetic map of the ISCfe1 insertion element and specific sites of insertion in: (a) the sodium/hydrogen exchanger protein gene nahE; (b) the putative methyltransferase protein gene metT; and (c) the putative VirB6 protein gene. Arrows dashed vertically and diagonally represent the genes of transposases A and B, respectively, of the insertion element ISCfe1. Inverted repeats flanking ISCfe1 are indicated with small white triangles. Grey arrows indicate genes that are affected by the insertion element ISCfe1. Small black arrows show the localisation of the primers used in the present study. Clinical Microbiology and Infection 2007 13, 993-1000DOI: (10.1111/j.1469-0691.2007.01787.x) Copyright © 2007 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 Detection of the ISCfe1 element. Southern blots of genomic DNA from Campylobacter fetus subsp. venerealis (CFV) and C. fetus subsp. fetus (CFF) strains cut with Bg/II and probed with digoxigenin-11-dUTP (DIG)-labelled ISCfe1. Strains analysed were as follows: (a) lane 1, type strain CCUG 538T; lane 2, CCUG 11287; lane 3, CCUG 7477; lane 4, CCUG 33900; lane 5, CCUG 33936; lane 6, CCUG 33902; lane 7, CCUG 33871; lane 8, CCUG 33872; lane 9, CCUG 24260; lane 10, CCUG 34335; lane 11, NZ 4266/95; lane 12, NZ 4267/95; lane 13, NZ 4268/95; lane 14, NZ 4269/95; lane 15, NZ 4270/95; lane 16, NZ 4271/95; lane 17, NZ 4272/95; lane 18, NZ 4273/95; lane 19, NZ 4274/95; lane 20, CCUG 34394; and lane 21, CCUG 34396; (b) lane 1, type strain NCTC 10354T; lane 2, NZ 4264/95; lane 3, CCUG 34395, lane 4, CCUG 33901; lane 5, CCUG 34111; lane 6, ATCC 25936; lane 7, CCUG 33671; lane 8, CCUG 32676; lane 9, CCUG 30605; lane 10, CCUG 30043; lane 11, K 34-1/91; and lane 12, K 49-2/91. M denotes molecular size standards. Arrows show highly conserved ISCfe1 element-positive bands. The presence of band 1 correlates with the ISCfe1 insertion into the sodium/hydrogen exchanger protein gene nahE. The presence of band 2 correlates with the ISCfe1 insertion into the putative methyltransferase protein gene metT. These two protein genes are present intact in CFF. Clinical Microbiology and Infection 2007 13, 993-1000DOI: (10.1111/j.1469-0691.2007.01787.x) Copyright © 2007 European Society of Clinical Infectious Diseases Terms and Conditions