Secretion efficiency of recombinant laminin-111, laminin-511, and their EQ mutants. Secretion efficiency of recombinant laminin-111, laminin-511, and their.

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Secretion efficiency of recombinant laminin-111, laminin-511, and their EQ mutants. Secretion efficiency of recombinant laminin-111, laminin-511, and their EQ mutants. SDS–PAGE under nonreducing conditions and subsequent immunoblot analyses of the conditioned media of human 293 cells transiently expressing laminin-111, laminin-511, or their EQ mutants. The anti–laminin-γ1 antibody detected secreted heterotrimeric laminins at around 800 kDa (arrowheads). Asterisks refer to the β1-γ1 dimer band. Conditioned medium of cells transfected with empty vectors (Mock) was also analyzed as a control. Daiji Kiyozumi et al. LSA 2018;1:e201800064 © 2018 Kiyozumi et al.