Structural rearrangements of ARF1 SBS upon hStau1 binding. Structural rearrangements of ARF1 SBS upon hStau1 binding. (A) Superposition of ARF1 SBS in its unbound state (red) and in complex with hStau1 (black), in the same orientations as in Fig 4A. Base pairs in the major groove interacting with helix α2 (region 3) of each dsRBD are indicated. (B)ARF1 SBS structural parameters calculated with w3DNA (Zheng et al, 2009). Twist: helical twist angle between consecutive C1′-C1′ vectors. Rise: helical rise by projection of the vector connecting consecutive C1′-C1′ middle points onto the helical axis. Minor and major groove widths: direct P–P distances, not accounting for Van der Waals radii of the phosphate groups. Graphical illustrations of the parameters are reproduced from http://x3dna.org (Lu & Olson, 2003). Differences between ARF1complex and ARF1apo values are calculated in the last column for each considered parameter; and lower values in ARF1complex are highlighted in red and higher values in blue (related to Fig 4). Daniela Lazzaretti et al. LSA 2018;1:e201800187 © 2018 Crown copyright. The government of Australia, Canada, or the UK ("the Crown") owns the copyright interests of authors who are government employees. The Crown Copyright is not transferable.