Location, morphology and gene expression of skin CD8α+MHC II+ cells.

Slides:

Advertisements

Similar presentations

Last Class Isolation of cells Cell Fraction, Centrifuge Chromatography

Advertisements

Volume 9, Issue 5, Pages (November 2017)

NK cells inhibited the expansion and function of ILC2s in vitro via IFN-γ. NK cells inhibited the expansion and function of ILC2s in vitro via IFN-γ. ILC2s.

Supporting information

Supplemental Figure 5 A B

DHEA(S) and Allo activated transcription factors NF-κB and CREB in serum-deprived PC12 cells. DHEA(S) and Allo activated transcription factors NF-κB and.

Uptake of T-MPs for DC maturation and antigen presentation.

Volume 132, Issue 2, Pages (February 2007)

Targeting lentiviral vector expression to hepatocytes limits transgene-specific immune response and establishes long-term expression of human antihemophilic.

by Dawne M. Page, Valerie Wittamer, Julien Y. Bertrand, Kanako L

Expression and regulation of MD-2s.

Jamaal L. Benjamin, Rhea Sumpter, Beth Levine, Lora V. Hooper

Immunologic responses after the MN-mediated cancer immunotherapy.

Localization of Calcineurin/NFAT in Human Skin and Psoriasis and Inhibition of Calcineurin/NFAT Activation in Human Keratinocytes by Cyclosporin A Wael.

IL-10-Producing Langerhans Cells and Regulatory T Cells Are Responsible for Depressed Contact Hypersensitivity in Grafted Skin Ryutaro Yoshiki, Kenji.

Volume 9, Issue 5, Pages (November 2017)

Inter-α inhibitor proteins maintain neutrophils in a resting state by regulating shape and reducing ROS production by Soe Soe Htwe, Hidenori Wake, Keyue.

Katrin Pauls, Margarete Schön, Robert C

Selection and Identification of Skeletal-Muscle-Targeted RNA Aptamers

Increased movement of GPR55 KO cells in association with epithelial cells. Increased movement of GPR55 KO cells in association with epithelial cells. (A)

Volume 37, Issue 5, Pages (November 2012)

Response of monocytes to hY4 delivery and cytokine levels in CLL serum

CD169+ macrophages mediate Lm translocation to the splenic T cell zones. CD169+ macrophages mediate Lm translocation to the splenic T cell zones. (A) Confocal.

Keratinocyte-Specific Deletion of the Receptor RAGE Modulates the Kinetics of Skin Inflammation In Vivo Julia S. Leibold, Astrid Riehl, Jan Hettinger,

TCR signaling induces nuclear actin polymerization.

TrkB-T1 is upregulated in cerebellum of Pex14ΔC/ΔC BL/ICR mouse at P3.

Epithelial Cells in the Hair Follicle Bulge do not Contribute to Epidermal Regeneration after Glucocorticoid-Induced Cutaneous Atrophy Dmitry V. Chebotaev,

BDNF expression in the cerebellum and brain stem region.

Jean Salamero, Bruno Goud Journal of Investigative Dermatology

Oral Tolerance Can Be Established via Gap Junction Transfer of Fed Antigens from CX3CR1+ Macrophages to CD103+ Dendritic Cells Elisa Mazzini, Lucia Massimiliano,

Volume 3, Issue 2, Pages (February 2006)

Rapamycin inhibits TCR/CD3 engagement-dependent HIF-1α protein expression. Rapamycin inhibits TCR/CD3 engagement-dependent HIF-1α protein expression. Human.

MAP Kinase Abnormalities in Hyperproliferative Cultured Fibroblasts from Psoriatic Skin Stéphanie Dimon-Gadal, Françoise Raynaud, Danièle Evain-Brion,

Molecular Therapy - Methods & Clinical Development

Characterization of immunosorted NCAM1+ subpopulation A

Expression of Activated MEK1 in Differentiating Epidermal Cells Is Sufficient to Generate Hyperproliferative and Inflammatory Skin Lesions Robin M. Hobbs,

Protection from autoimmunity is not due to the expansion of Treg subsets. Protection from autoimmunity is not due to the expansion of Treg subsets. (A.

Transcriptional Repression of miR-34 Family Contributes to p63-Mediated Cell Cycle Progression in Epidermal Cells Dario Antonini, Monia T. Russo, Laura.

Figure 2 Induced deletion of CXCR2 on oligodendrocyte lineage cells after tamoxifen injection in Cxcr2-cKO mice Induced deletion of CXCR2 on oligodendrocyte.

Thilo Jakob, Mark C. Udey Journal of Investigative Dermatology

Cell-Shape Regulation of Smooth Muscle Cell Proliferation

Occurrence of prominin-1-containing particles in the ventricular fluid of the embryonic mouse brain. Occurrence of prominin-1-containing particles in the.

IL-10R-deficient macrophages secrete IL-23, inducing IL-22 secretion by ILC3 and TH17 cells. IL-10R-deficient macrophages secrete IL-23, inducing IL-22.

Fig. 5. Expression of either dFMRP or human FMRP does not induce eIF2α phosphorylation.(A) Analysis of eIF2α phosphorylation upon dFMRP expression. Expression.

Fig. 3. Characterization of unclassified cells (UCs).

PD-L1 expression is maintained on NCMs under inflammatory conditions and PD-L1+NCMs are found in TLOs. PD-L1 expression is maintained on NCMs under inflammatory.

Activation of Keratinocyte Protein Kinase Cζ in Psoriasis Plaques

CD169+ macrophages mediate the transport of bacteria to T cell zones by trans-infecting CD8α+ DCs. CD169+ macrophages mediate the transport of bacteria.

Exo70 is recruited to the plasma membrane at sites of mechanical wounding. Exo70 is recruited to the plasma membrane at sites of mechanical wounding. NRK.

Fig. 3 BIC1 and BIC2 inhibit early photoreactions of cryptochromes.

Quantitative evaluation of isolated Calliphora rhabdoms labelled for Rh3, Rh5 and Rh6. Quantitative evaluation of isolated Calliphora rhabdoms labelled.

Expression of p110γ isoform in macrophages promotes tumor growth and angiogenesis. Expression of p110γ isoform in macrophages promotes tumor growth and.

Characterization of NEX-CRE mice and analysis of Itgb1 expression by flow cytometry. a–n , Z/EG reporter mice carrying a CRE-inducible GFP transgene were.

Fibril-induced NM prions do not evolve from SGs.

Establishment of OL1 cells.

CD25 expression identifies two transcriptionally distinct subsets of very early effector cells. CD25 expression identifies two transcriptionally distinct.

Morphologic changes induced in immortalized human podocytes after treatment with TGF-β1 (10 ng/mL, right) for 3 days when compared with control cells (left),

GABAARs in RFMes GABAergic neurons across groups.

Geldanamycin-induced endocytosis and down-regulation of ErbB2 in cells expressing ErbB2 only. Geldanamycin-induced endocytosis and down-regulation of ErbB2.

OPN mediates maturation from immature DCs to mature DCs

Fig. 2 IRF8 is expressed in CD68+ macrophages after SCI.

Dysregulated NF-κB activation in Il1r8+/+/lpr and Il1r8−/−/lpr mice.

Activation of Vγ9 T cells by three distinct stimuli is CD277 dependent

Expression of B7-H1, B7-DC, and PD-1 on B cells.

Expression of PCNA, K10, and K5 in skin lesions from Stat3+/−:HPV8 and Stat3+/+:HPV8 mice. Expression of PCNA, K10, and K5 in skin lesions from Stat3+/−:HPV8.

Expression of IFN-λs, IFN-λR1, and IL-10R2 in Xenopus tissues after stimulation in vivo with poly(I:C). Expression of IFN-λs, IFN-λR1, and IL-10R2 in Xenopus.

CpG oligodeoxynucleotide (CpG ODN)–induced activation of different types of primary malignant B cells. CpG oligodeoxynucleotide (CpG ODN)–induced activation.

Analysis of absolute CD4+ cytokine+ cell numbers pre- and post-ART.

Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization in 231BR3 cells. Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization.

Microscopic imaging of different C

Presentation transcript:

Location, morphology and gene expression of skin CD8α+MHC II+ cells. Location, morphology and gene expression of skin CD8α+MHC II+ cells. (A) For immunofluorescence analysis, cryostat sections with a thickness of 20 μm were prepared from rainbow trout skin, fixed, and labeled with anti-CD8α (red) and anti–MHC class II (green) Abs, counterstained with Hoechst (blue), and analyzed by confocal microscopy. Arrowheads show double positive cells. Scale bars, 20 μm. (B) For identification of CD8α+MHC II+ cells, total leukocytes from skin were fixed and labeled with anti-CD8α (green) and anti–MHC class II (red) Abs, counterstained with Hoechst (blue), and analyzed by fluorescence microscopy. Scale bars, 5 μm. (C) CD8α+MHC II+ cells from skin were isolated by cell sorting and then incubated onto poly-l-lysine–treated glass slides, fixed, mounted, and analyzed by light microscopy. Scale bar, 5 μm. (D) Myeloid CD8α+MHC II+ cells from skin also were analyzed using a scanning electron microscope. Scale bar, 5 μm. (E) Sorted myeloid skin CD8α+MHC II+ cells were analyzed by real time-PCR. Relative expression of the indicated genes to the endogenous control EF-1α was calculated for each sample, and mean (± SD) values from three independent experiments, including three fish per experiment, were obtained. Aitor G. Granja et al. J Immunol 2015;195:1825-1837 Copyright © 2015 by The American Association of Immunologists, Inc.