V-SVZ cultures express transcription factors expected for region of origin. V-SVZ cultures express transcription factors expected for region of origin.

Slides:

Advertisements

Similar presentations

Sami Boudkkazi, Aline Brechet, Jochen Schwenk, Bernd Fakler Neuron

Advertisements

Modeling human tumor angiogenesis in a three-dimensional culture system by Giorgio Seano, Giulia Chiaverina, Paolo Armando Gagliardi, Laura di Blasio,

STAU1/2 and YBX1 recruit poly-GR/PR into large cytoplasmic granules.

NPM1 drives poly-GR into the nucleolus of primary neurons.

Poly-GR and poly-PR co-aggregate with ribosomal proteins in C9orf72 patients. Poly-GR and poly-PR co-aggregate with ribosomal proteins in C9orf72 patients.

Roger B. Deal, Steven Henikoff Developmental Cell

Characterization of DPR proteins in primary rat neurons and HEK293 cells. Characterization of DPR proteins in primary rat neurons and HEK293 cells. GFP,

Chd5 deficiency in NSCs leads to premature activation.

Expression of FUT9 supports tumor development

Fig. 4. Specific versus nonspecific NP accumulation.

Volume 10, Issue 4, Pages (April 2018)

Validation of astrocyte and microglial proteomic markers that increase across the ALS‐FTD spectrum Validation of astrocyte and microglial proteomic markers.

CD169+ macrophages play a critical role in mediating innate immune cell reorganization. CD169+ macrophages play a critical role in mediating innate immune.

Perioperative assessment of regional ventilation during changing body positions and ventilation conditions by electrical impedance tomography A. Ukere,

Selection and Identification of Skeletal-Muscle-Targeted RNA Aptamers

Nucleolar poly-GR/PR alter nucleolar organization and inhibit translation. Nucleolar poly-GR/PR alter nucleolar organization and inhibit translation. GFP,

Candidates with a PVM localization.

IL-27–eGFP is highly expressed by cDC1 cells and Ly6Chi monocytes after vaccine adjuvant administration. IL-27–eGFP is highly expressed by cDC1 cells and.

PD-1 expression on HCC-infiltrating B cells and its clinical significance. PD-1 expression on HCC-infiltrating B cells and its clinical significance. A–H,

Fig. 5. pKL cells abrogate the autoimmune response in vitro.

Abdul Q. Sheikh, Janet K. Lighthouse, Daniel M. Greif Cell Reports

CD169+ macrophages mediate Lm translocation to the splenic T cell zones. CD169+ macrophages mediate Lm translocation to the splenic T cell zones. (A) Confocal.

TrkB-T1 is upregulated in cerebellum of Pex14ΔC/ΔC BL/ICR mouse at P3.

Isabelle Plaisance et al. BTS 2016;j.jacbts

Axonal swelling and impairment of dendritic development in Purkinje cells from Pex14ΔC/ΔC BL/ICR mouse upon treatment with BDNF. Axonal swelling and impairment.

Marker identification and quantification by StrataQuest, and confocal analysis of EV-containing tissue sections. Marker identification and quantification.

Mice with Wt1 inactivation display altered locomotion.

BDNF expression in the cerebellum and brain stem region.

A. A. The two independent mutations identified in the structural gene of pqn-82 are shown above the gene. The genomic coordinates for the altered base.

KIF18A sNL variants do not accumulate on K-fibers in the center of the spindle. KIF18A sNL variants do not accumulate on K-fibers in the center of the.

Volume 16, Issue 5, Pages (August 2016)

Validation of astrocyte and microglial proteomic markers that increase across the ALS‐FTD spectrum Validation of astrocyte and microglial proteomic markers.

B cells infiltrate mouse and human pancreatic neoplasia and promote growth of KrasG12D-PDEC in vivo. B cells infiltrate mouse and human pancreatic neoplasia.

Localization of PBANKA_ and PBANKA_ during blood and liver stage development. Localization of PBANKA_ and PBANKA_ during blood.

ILK knockdown decreases mTOR signaling in PKD kidneys.

Quantification of MHC-I, β2m, and T-cell subsets.

Nuclear DNA signal is altered in Chd5-deficient NSCs.

Ab-dependent synaptic loss and neuritic dystrophies in adult APP/PSEN1 and APP/PSEN1/APOEnull mice. Ab-dependent synaptic loss and neuritic dystrophies.

p-S6 S240/244 is observed across the V-SVZ lineage.

Transcription factors defining dorsal or ventral identity are differentially expressed in dorsal and ventral hamartomas in TSC. Transcription factors defining.

No correlation between the distance of the duplicated alleles and the transcription levels on each of the alleles. No correlation between the distance.

Altered oligodendrocyte progenitor cell (OPC) marker protein expression in two white matter regions of ShhΔFP/ΔFP and SmoΔNT/ΔNT embryos at E15.5. Altered.

Variation in the Dorsal Gradient Distribution Is a Source for Modified Scaling of Germ Layers in Drosophila Juan Sebastian Chahda, Rui Sousa-Neves, Claudia Mieko.

MET/β-catenin complexes during synapse development.

Chd5 deficiency leads to compromised expression of the repressive histone mark H3K27me3 and up-regulation of ribosomal protein genes. Chd5 deficiency leads.

Volume 9, Pages (November 2018)

Socs1 KD tumors exhibit a more T-cell–inflamed phenotype and increased T-cell activation. Socs1 KD tumors exhibit a more T-cell–inflamed phenotype and.

Volume 9, Issue 6, Pages (December 2014)

Ventral mouse V-SVZ cells exhibit higher mTORC1 activity than dorsal cells and are responsive to rapamycin. Ventral mouse V-SVZ cells exhibit higher mTORC1.

Fluorescence flow cytometry shows no difference in total protein levels but reveals a slight difference in cell size. Fluorescence flow cytometry shows.

Fgfr3;4 mutant lungs display an increase in Mfap5, Igf1 and Fbn2 expression. Fgfr3;4 mutant lungs display an increase in Mfap5,Igf1and Fbn2 expression.

Sustained PGC-1α loss of function triggers mesenchymal transition.

The TAP population exhibits the highest mTORC1 signaling differences between dorsal and ventral regions. The TAP population exhibits the highest mTORC1.

Global cell proliferation replenishes epidermal cells.

Chd5-deficient NSCs generate excessive astrocytes at the expense of neurons. Chd5-deficient NSCs generate excessive astrocytes at the expense of neurons.

Effect of M-cadherin RNAi on apoptosis in confluent C2C12 myoblasts.

Volume 9, Issue 6, Pages (December 2014)

PTZ-induced c-Fos and Arc proteins in hippocampal dentate gyrus

S-Affs colocalize with SUMO in mammalian cells.

Optogenetic analysis of mPFC neurons.

Fig. 1. Matrix stiffness sensitizes myofibroblasts to apoptosis induced by inhibition of their mechanotransduction pathways. Matrix stiffness sensitizes.

Long noncoding RNAs are distributed throughout the genome and are characteristically distinct from annotated mRNAs. Long noncoding RNAs are distributed.

TAMs directly contribute to tumor hypoxia.

The CCR5+ population of SUM-159 cells is enriched with tumor-initiating cells. The CCR5+ population of SUM-159 cells is enriched with tumor-initiating.

IFN treatment of human midgestation villous explants induces syncytial knot formation. IFN treatment of human midgestation villous explants induces syncytial.

Fig. 5 Reconstitution of the dormant state using fetal ovaries.

Integrated analysis of gene expression and copy number alterations.

Sami Boudkkazi, Aline Brechet, Jochen Schwenk, Bernd Fakler Neuron

Apical junctions restrict RNA virus infection of 3-D-cultured HBMEC

Spontaneous PD in DRB1*04:01 mice.

Presentation transcript:

V-SVZ cultures express transcription factors expected for region of origin. V-SVZ cultures express transcription factors expected for region of origin. (A) Cartoon schematic of culture preparation. (B) Representative confocal images of P2 V-SVZ cultures stained for DAPI (blue), NKX2.1 (red), and PAX6 (green) protein. PAX6 and NKX2.1 staining are shown in grayscale to the right. Quantification of positive nuclei in dorsal and ventral cultures is displayed as box and whisker plots to the right; bars, median + 25th and 75th percentile ± SD. Scale bars, 50 μm. n = 6 mice, 3 ROIs per mouse. Paired t tests, P < 0.001 (Pax6), P = 0.007 (Nkx2.1). (C) Graph showing transcript abundance for the transcription factors NKX2.1, NKX6.2, and PAX6 from P2 V-SVZ cultures. Ventral ΔCT is subtracted from Dorsal ΔCT; therefore, transcripts higher in dorsal samples are above 0 and transcripts higher in ventral samples are below 0. N = 5 mice, CT values measured in triplicate, normalized to Ubc. Paired t tests dorsal versus ventral, P = 0.0043 (NKX2.1), P = 0.0028 (NKX6.2), P = 0.0260 (PAX6). Gabrielle V Rushing et al. LSA 2019;2:e201800218 © 2019 Rushing et al.