Volume 10, Issue 3, Pages (March 2017)

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Volume 10, Issue 3, Pages 520-522 (March 2017) A 4-bp Insertion at ZmPLA1 Encoding a Putative Phospholipase A Generates Haploid Induction in Maize Chenxu Liu, Xiang Li, Dexuan Meng, Yu Zhong, Chen Chen, Xin Dong, Xiaowei Xu, Baojian Chen, Wei Li, Liang Li, Xiaolong Tian, Haiming Zhao, Weibin Song, Haishan Luo, Qinghua Zhang, Jinsheng Lai, Weiwei Jin, Jianbing Yan, Shaojiang Chen Molecular Plant Volume 10, Issue 3, Pages 520-522 (March 2017) DOI: 10.1016/j.molp.2017.01.011 Copyright © 2017 The Authors Terms and Conditions

Figure 1 ZmPLA1 (GRMZM2G471240) Mutation Causes Haploid Induction in Maize. (A) Genetic mapping for qhir1. Fine mapping identified a 243-kb region containing three expressed genes, a coding gene GRMZM2G471240 (in red), and two long noncoding RNAs (lncRNA1 and 2; in gray). (B) The expression pattern of GRMZM2G471240 in B73 and B73-inducer line at meiosis and one-nucleus, two-nuclei, and three-nuclei stages of pollen development. (C) The structure of GRMZM2G471240 with 4-bp insertion (at red triangle). The red arrow shows the CRISPR target site in the first exon of GRMZM2G471240. The insertion and deletion sites of three allelic mutations (ZmHIR1-1, ZmHIR1-2, ZmHIR1-3) are shown in the alignment comparison with B73 sequence. (D) The phenotype of wild (receptor line) and three T1 generation (ZmHIR1-1, ZmHIR1-2, ZmHIR1-3) self-cross ears. Endosperm abortion kernels were detected in the three T1 generation knockout ears. (E) The rate of endosperm aborted kernels showed a significant difference between wild-type and T1 generation knockout plants, and also in their hybrid plants with ZD958 and JK968. I, selfing-T1; II, receptor inbred line (wild-type); III, ZD958 × T1; IV, ZD958 × receptor inbred line (wild-type); V, JK968 × T1; VI, JK968 × receptor inbred line (wild-type). **p < 0.01. (F) Field performance of diploid (left) and haploid (right) plants from the progeny of ZD958 pollinated by using T1 knockout plants as male. (G) The anther phenotypes of diploid (left) and haploid (right) plants from the progenies of ZD958 pollinated by T1 knockout plants as male. (H) Flow cytometry results of diploid (left) and haploid (right) DNA (signal intensity values indicated). (I) PCR testing of diploid (left) and haploid plants (right) using polymorphic simple sequence repeat markers. Left-1, JK968 as female; left-2 (diploid), F1 between JK968 and knockout T1 plant; left-3, knockout T1 plant as male. Right-1, JK968 as female; right-2 (haploid), F1 between JK968 and knockout T1 plant; right-3, knockout T1 plant as male. Primer information is provided in Supplemental Table 3. Molecular Plant 2017 10, 520-522DOI: (10.1016/j.molp.2017.01.011) Copyright © 2017 The Authors Terms and Conditions