MAPK14 regulates ROS generation in GS cells.

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MAPK14 regulates ROS generation in GS cells. MAPK14 regulates ROS generation in GS cells. (A) Real-time PCR analysis of Mapk11, Mapk12, Mapk13, and Mapk14 in GS cells (n = 3 cultures). *P < 0.05 (ANOVA). (B) Appearance of GS cells after culturing with VX-745 for 6 d. Scale bar: 100 μm. (C) Cell recovery (n = 9 cultures). GS cells were cultured with VX-745 for 6 d. *P < 0.05 (ANOVA). (D) Flow cytometric analysis of ROS generation 5 d after VX-745 supplementation. (n = 3 cultures). *P < 0.05 (linear regression). (E) Western blot analysis of phosphorylated MAPK14 10 min after hydrogen peroxide (30 μM) supplementation. (F) Western blot analysis of phosphorylated MAPK14 2 d after NAC (0.5 mM) supplementation. (G) Flow cytometric analysis of ROS generation 1 d after transfection with constitutively active Mapk14 (n = 3 cultures; multiplicity of infection [moi] = 4). *P < 0.05 (linear regression). (H) Real-time PCR analysis of Nox1 expression 1 d after transfection with constitutively active Mapk14 (moi = 4; n = 5 cultures). *P < 0.05 (t test). Data information: in (A, C, D, G, and H), data are presented as mean ± SEM. Hiroko Morimoto et al. LSA 2019;2:e201900374 © 2019 Morimoto et al.