Volume 56, Issue 1, Pages (July 1999)

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Presentation transcript:

Volume 56, Issue 1, Pages 338-343 (July 1999) An automated technique for the simultaneous determination of cations in nanoliter volumes Christopher J. Stocking, Jonathan M. Slater, Robert Unwin, Stephen Walter, Elizabeth Folkerd Kidney International Volume 56, Issue 1, Pages 338-343 (July 1999) DOI: 10.1046/j.1523-1755.1999.00525.x Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 1 Schematic diagram of the capillary electrophoresis system. Kidney International 1999 56, 338-343DOI: (10.1046/j.1523-1755.1999.00525.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 2 Calibration curve for sodium. The solid line represents the regression and the dotted lines represent the 95% confidence interval (Y = -1.23418 + 0.916838x; r2 = 1.000). Kidney International 1999 56, 338-343DOI: (10.1046/j.1523-1755.1999.00525.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 3 Calibration curve for potassium. The solid line represents the regression and the dotted lines represent the 95% confidence interval (Y = -8.6E-02 + 1.02006x; r2 = 0.998). Kidney International 1999 56, 338-343DOI: (10.1046/j.1523-1755.1999.00525.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 4 Electropherogram of cations in standard material, (1) ammonium, (2) potassium, (3) sodium, (4) calcium, (5) magnesium, (6) barium, and (7) lithium. Capillary: ConCap 50 μm i.d. 53 cm length. Kidney International 1999 56, 338-343DOI: (10.1046/j.1523-1755.1999.00525.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 5 Electropherogram of cations in a sample from the distal tubule. (1) ammonium, (2) potassium, (3) sodium, and (4) calcium. Capillary: ConCap 50 μm i.d. 40 cm length. Kidney International 1999 56, 338-343DOI: (10.1046/j.1523-1755.1999.00525.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 6 Comparison of sodium values with those obtained by atomic absorption (AA). The solid line represents the regression (Y = -1.58724 + 1.01219x; r2 = 0.974). Kidney International 1999 56, 338-343DOI: (10.1046/j.1523-1755.1999.00525.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 7 Comparison of potassium values with those obtained by atomic absorption (AA). The solid line represents the regression (Y = -0.326357 + 0.978315x; r2 = 0.963). Kidney International 1999 56, 338-343DOI: (10.1046/j.1523-1755.1999.00525.x) Copyright © 1999 International Society of Nephrology Terms and Conditions