Basophil and eosinophil accumulation and mast cell degranulation in the nasal mucosa of patients with hay fever after local allergen provocation Alex.

Slides:

Advertisements

Similar presentations

Assessment of inflammation in noninfectious chronic maxillary sinusitis Pascal Demoly, MDa, Louis Crampette, MDb, Michel Mondain, MDb, Alison M. Campbell,

Advertisements

Intradermal grass pollen immunotherapy increases TH2 and IgE responses and worsens respiratory allergic symptoms Anna Slovick, MRCS, Abdel Douiri, PhD,

Allergen-induced expression of IL-25 and IL-25 receptor in atopic asthmatic airways and late-phase cutaneous responses Chris J. Corrigan, MD, PhD, Wei.

F.Estelle R. Simons, MDa, Bruce M. Prenner, MDb, Albert Finn, MDc

Response to cyclosporin and low-dose methylprednisolone in aggressive systemic mastocytosis Motohiro Kurosawa, MD, PhD, PharmDa, Hiroo Amano, MD, PhDb,

Gene expression of vascular endothelial growth factor and its receptors and angiogenesis in bronchial asthma Makoto Hoshino, MDa, Yutaka Nakamura, MDa,

Ryan H. Dougherty, MD, Sukhvinder S

Allergic Rhinitis and Its Impact on Asthma

Calcitonin gene-related peptide– and vascular endothelial growth factor–positive inflammatory cells in late-phase allergic skin reactions in atopic subjects

Heterogeneity of mast cells in mastocytosis and inhibitory effect of ketotifen and ranitidine on indolent systemic mastocytosis Motohiro Kurosawa, MD,

Hong Wei Chu, MDa, Monica Kraft, MDa, James E. Krause, PhDb, Michael D

Rapid recruitment of CD14+ monocytes in experimentally induced allergic rhinitis in human subjects Ibon Eguíluz-Gracia, MD, Anthony Bosco, PhD, Ralph.

The effect of omalizumab on nasal allergic inflammation

Comparison of once-daily ebastine 20 mg, ebastine 10 mg, loratadine 10 mg, and placebo in the treatment of seasonal allergic rhinitis Paul H. Ratner,

Dose-dependent effects of capsaicin nasal challenge: In vivo evidence of human airway neurogenic inflammation Alvin M. Sanico, MDa, Satsuki Atsuta, MDa,

Allergen binding to specific IgE in the nasal mucosa of allergic patients Alex KleinJan, BSca, Tom Godthelp, MDa, Albert van Toornenenbergen, PhDb, Wytske.

Youngil I. Koh, MD a, Inseon S

Grass pollen immunotherapy inhibits allergen-induced infiltration of CD4+ T lymphocytes and eosinophils in the nasal mucosa and increases the number of.

Pathophysiology of severe asthma

Inflammation of small airways in asthma

Allergen-induced expression of IL-25 and IL-25 receptor in atopic asthmatic airways and late-phase cutaneous responses Chris J. Corrigan, MD, PhD, Wei.

Comparison of the efficacy of budesonide and fluticasone propionate aqueous nasal spray for once daily treatment of perennial allergic rhinitis James.

Comparison of a nasal glucocorticoid, antileukotriene, and a combination of antileukotriene and antihistamine in the treatment of seasonal allergic rhinitis

Intradermal grass pollen immunotherapy increases TH2 and IgE responses and worsens respiratory allergic symptoms Anna Slovick, MRCS, Abdel Douiri, PhD,

Cellular and mediator responses twenty-four hours after local endobronchial allergen challenge of asthmatic airways Anthony J. Frew, MD, Johanne St-Pierre,

Jesús Merayo-Lloves, MD, Tong Z. Zhao, MD, James E. Dutt, BA, C

Volume 59, Issue 4, Pages (April 2001)

Peanut-induced intestinal allergy is mediated through a mast cell–IgE–FcεRI–IL-13 pathway Meiqin Wang, MD, PhD, Katsuyuki Takeda, MD, PhD, Yoshiki Shiraishi,

Distinguishing severe asthma phenotypes

Evidence for expression of eosinophil-associated IL-12 messenger RNA and immunoreactivity in bronchial asthma Esra Nutku, MDa, Abdelilah Soussi Gounni,

Fluticasone propionate aqueous nasal spray reduces inflammatory cells in unchallenged allergic nasal mucosa: Effects of single allergen challenge Adriaan.

The role of the mast cell in the pathophysiology of asthma

Local allergic reaction in food-hypersensitive adults despite a lack of systemic food- specific IgE Xiao Ping Lin, MD a, Jenny Magnusson, BSc a,b, Staffan.

Basophils and eosinophils in allergic rhinitis

Barbara Foster, MSa, Lawrence B

CC chemokine receptors CCR1 and CCR4 are expressed on airway mast cells in allergic asthma Kawa Amin, PhD, Christer Janson, MD, PhD, Ilkka Harvima, MD,

Inflammatory cell and epithelial characteristics of perennial allergic and nonallergic rhinitis with a symptom history of 1 to 3 years’ duration Kawa.

Subjective and objective assessments in patients with seasonal allergic rhinitis: Effects of therapy with mometasone furoate nasal spray Eli O. Meltzer,

Nasal eosinophilia and IL-5 mRNA expression in seasonal allergic rhinitis induced by natural allergen exposure: Effect of topical corticosteroids Keisuke.

F.Estelle R. Simons, MDa, Bruce M. Prenner, MDb, Albert Finn, MDc

Classification of anti-FcϵRI and anti-IgE autoantibodies in chronic idiopathic urticaria and correlation with disease severity Ruth A. Sabroe, MDa, Edda.

Corticosteroid-induced apoptosis in mouse airway epithelium: Effect in normal airways and after allergen-induced airway inflammation Delbert R. Dorscheid,

Double-blind placebo-controlled evaluation of sublingual-swallow immunotherapy with standardized Parietaria judaica extract in children with allergic.

TH1/TH2 cytokines and inflammatory cells in skin biopsy specimens from patients with chronic idiopathic urticaria: Comparison with the allergen-induced.

Assessment of inflammation in noninfectious chronic maxillary sinusitis Pascal Demoly, MDa, Louis Crampette, MDb, Michel Mondain, MDb, Alison M. Campbell,

Loratadine reduces allergen-induced mucosal output of α2-macroglobulin and tryptase in allergic rhinitis Lennart Greiff, MD, PhDa, Carl G.A. Persson,

Ligation of IgE receptors causes an anaphylactic response and neutrophil infiltration but does not induce eosinophilic inflammation in mice Masayuki.

Nasal polyposis: Immunohistochemistry and bioelectrical findings (a hypothesis for the development of nasal polyps) Joel M. Bernstein, MD, PhDa, Janet.

Seasonal intestinal inflammation in patients with birch pollen allergy

Nasal inflammatory mediators and specific IgE production after nasal challenge with grass pollen in local allergic rhinitis Carmen Rondón, MD, PhD, Javier.

Esophageal eosinophilia caused by milk proteins: From suspicion to evidence based on 2 case reports Soledad Terrados Cepeda, MD, Dario Antolin-Amerigo,

Nasal allergen provocation induces adhesion molecule expression and tissue eosinophilia in upper and lower airways Gert-Jan Braunstahl, MDa, b, Shelley.

Exposure to cow’s milk during the first 3 months of life is associated with increased levels of IgG subclass antibodies to β-lactoglobulin to 8 years

Rame A. Taha, MDa, Eleanor M. Minshall, PhDa, Donald Y. M

Monocyte chemotactic proteins in allergen-induced inflammation in the nasal mucosa: Effect of topical corticosteroids Pota Christodoulopoulos, BSca,

Local expression of ϵ germline gene transcripts and RNA for the ϵ heavy chain of IgE in the bronchial mucosa in atopic and nonatopic asthma Sun Ying,

Jonathan M. Corne, MRCPa, Catherine H. Linaker, BNa, Peter H

Cytokine profile in minor salivary glands from patients with bronchial asthma Anne Tsicopoulos, MDa, b, Anne Janin, MDc, Hikmat Akoum, PhDa, Catherine.

IL-4– and IL-5–positive T lymphocytes, eosinophils, and mast cells in allergen-induced late-phase cutaneous reactions in atopic subjects Luis T. Barata,

Clinical and immunologic variables in skin of patients with atopic eczema and either positive or negative atopy patch test reactions Elisabeth G. Langeveld-Wildschut,

IL-9 and c-Kit+ mast cells in allergic rhinitis during seasonal allergen exposure: Effect of immunotherapy Kayhan T. Nouri-Aria, PhD, FRCPath, Charles.

Allergic fungal sinusitis: An immunohistologic analysis

Alex KleinJan, PhD, Monique Willart, BSc, Leonie S

Successful treatment of eosinophilic gastroenteritis with suplatast tosilate Toshihiro Shirai, MD, Dai Hashimoto, MD, Kenichiro Suzuki, MD, Satoru Osawa,

Mechanism of topical glucocorticoid treatment of hay fever: IL-5 and eosinophil activation during natural allergen exposure are suppressed, but IL-4,

Dendritic cells in nasal mucosa of subjects with different allergic sensitizations Susanne M. Reinartz, MD, Joost van Tongeren, MD, Danielle van Egmond,

Concomitant montelukast and loratadine as treatment for seasonal allergic rhinitis: A randomized, placebo-controlled clinical trial Eli O. Meltzer, MDa,

Mast cell–associated alveolar inflammation in patients with atopic uncontrolled asthma Cecilia K. Andersson, PhD, Anders Bergqvist, MSc, Michiko Mori,

Mast cells infiltrate the esophageal smooth muscle in patients with eosinophilic esophagitis, express TGF-β1, and increase esophageal smooth muscle contraction

Advances in mechanisms of allergic disease in 2016

Presentation transcript:

Basophil and eosinophil accumulation and mast cell degranulation in the nasal mucosa of patients with hay fever after local allergen provocation Alex KleinJan, BSca, Alan R. McEuen, PhDb, Mariska D. Dijkstra, MDa, Mark G. Buckley, PhDb, Andrew F. Walls, PhDb, Wytske J. Fokkens, MD, PhDa Journal of Allergy and Clinical Immunology Volume 106, Issue 4, Pages 677-686 (October 2000) DOI: 10.1067/mai.2000.109621 Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 1 Total symptom score before and after allergen provocation. After both provocations (15 and 30 minutes), symptoms including sneezing, rhinorrhea, itching, and nasal blockage were recorded. Patients were also asked to rate their symptoms on a 4-point (0-3) scale during the day after provocation after 3, 6, 9, 12, and 24 hours. Journal of Allergy and Clinical Immunology 2000 106, 677-686DOI: (10.1067/mai.2000.109621) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 2 Photomicrograph of BB1-positive cells (red) in the epithelium and in the lamina propria of a nasal mucosal biopsy section obtained from an allergic patient before (A) and 24 hours after (B) allergen provocation. For tryptase, specimens are shown from before (C) and 24 hours after (D) allergen provocation, and for chymase, specimens are shown before (E) and 24 hours after (F) allergen provocation (counterstained with Gill’s hematoxylin; magnification ×1000). Journal of Allergy and Clinical Immunology 2000 106, 677-686DOI: (10.1067/mai.2000.109621) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 3 Numbers (in cells per square millimeter) of basophils (A and B ), tryptase-positive cells (C and D ), and chymase-positive cells (E and F ) in the epithelium (A , C , and E ) and lamina propria (B , D , and F ) in nasal mucosa biopsy specimens. Nasal mucosa biopsy specimens were taken before provocation, 1 hour after provocation, 24 hours after provocation, and 1 week after provocation. Allergen challenge (arrows) was performed with 1000 and 10,000 BU grass pollen. Box plots represent the 25th-75th percentile, the range (whiskers) , and the median value. Journal of Allergy and Clinical Immunology 2000 106, 677-686DOI: (10.1067/mai.2000.109621) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 4 Mast cells. Representative sections of double staining showing tryptase (red), chymase (blue), and tryptase-chymase double-positive cells (red and blue) indicate 3 types of mast cells: tryptase-positive (MCT) , tryptase-chymase positive (MCTC) , and chymase-positive (MCC) . a, Before provocation; b, after provocation (counterstaining with methyl-green; magnification ×1000). Journal of Allergy and Clinical Immunology 2000 106, 677-686DOI: (10.1067/mai.2000.109621) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 5 The numbers (A , C , and E) and proportion (B , D , and F) of tryptase-positive mast cell (MCT) phenotype (A and B) , chymase-positive mast cell (MCC) phenotype (C , D) , and tryptase-chymase double-positive mast cell (MCTC) phenotype (E and F). Journal of Allergy and Clinical Immunology 2000 106, 677-686DOI: (10.1067/mai.2000.109621) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 6 Numbers (in cells per square millimeter) of eosinophils (A and B ) and IgE-positive cells (C and D ) in the epithelium (A and C ) and lamina propria (B and D ) in nasal mucosa biopsy specimens. Nasal mucosa biopsy specimens were taken before provocation, 1 hour after provocation, 24 hours after provocation, and 1 week after provocation. Allergen challenge (arrows) was performed with 1000 and 10,000 BU grass pollen. Box plots represent the 25th-75th percentile, the range (whiskers) , and the median value. Journal of Allergy and Clinical Immunology 2000 106, 677-686DOI: (10.1067/mai.2000.109621) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 7 The inflammation of mast cells, eosinophils, and basophils related to the total inflammation of these cells in the lamina propria over time. Journal of Allergy and Clinical Immunology 2000 106, 677-686DOI: (10.1067/mai.2000.109621) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 8 Tryptase-positive basophils. Double staining showed tryptase (red) and BB1 (blue) double-positive cells (counterstained methyl-green; magnification ×1000). Double staining with CD18, tryptase, and BB1 indicates that BB1-positive cells are positive for tryptase. Journal of Allergy and Clinical Immunology 2000 106, 677-686DOI: (10.1067/mai.2000.109621) Copyright © 2000 Mosby, Inc. Terms and Conditions