Fertility and Sterility

Slides:

Advertisements

Similar presentations

Binding of recombinant human proacrosin/acrosin to zona pellucida (ZP) glycoproteins. I. Studies with recombinant human ZPA, ZPB, and ZPC Laura I. Furlong,

Advertisements

Calcium requirements for human sperm function in vitro

Identification and validation of candidate biomarkers involved in human ovarian autoimmunity Purvi V. Mande, Firuza R. Parikh, Indira Hinduja, Kusum.

Identification and validation of candidate biomarkers involved in human ovarian autoimmunity Purvi V. Mande, Firuza R. Parikh, Indira Hinduja, Kusum.

Effects of testosterone, dihydrotestosterone, and 17β-estradiol on human ovarian tissue survival in culture Marjut Otala, M.Sc., Sirpa Mäkinen, M.Sc.,

Wheat α-amylase inhibitor: A second route of allergic sensitization

Christopher L. Kepley, PhD, Bridget S. Wilson, PhD, Janet M

Identification of a novel sperm motility–stimulating protein from caprine serum: its characterization and functional significance Sudipta Saha, Ph.D.,

Ruben D. Motrich, M. Sc. , Andres A. Ponce, Ph. D. , Virginia E

Ana Maria Cuervo, Heinz Hildebrand, Ernst M. Bomhard, J. Fred Dice

Requirement of heat shock protein 90 in mesangial cell mitogenesis

Fertility and Sterility

Resistin decreases insulin-like growth factor I–induced steroid production and insulin- like growth factor I receptor signaling in human granulosa cells

Yihan Wang, Michael A. Shia, Thomas G. Christensen, Steven C. Borkan

Anti-ACTL7a antibodies: a cause of infertility

Volume 58, Issue 5, Pages (November 2000)

Members of the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway are present and active in human sperm Osmond J D’Cruz,

Hsien-An Pan, M. D. , Ph. D. , Yeu-Chin Lee, M. Sc. , Yen-Ni Teng, Ph

Rod outer segment membrane guanylate cyclase type 1 (ROS-GC1) calcium-modulated transduction system in the sperm Anna Jankowska, Ph.D., Beata Burczyńska,

Allergy to eggplant (Solanum melongena)

Hendri H. Pas, Guus J. Kloosterhuis, Miranda Nijenhuis, Marcelus C. J

Fas ligand+ fallopian tube epithelium induces apoptosis in both Fas receptor+ T lymphocytes and endometrial cells Sebastian E. Illanes, M.D., Kevin Maisey,

Marta Gianzo, J. D. , Iraia Muñoa-Hoyos, J. D

Binding of recombinant human proacrosin/acrosin to zona pellucida (ZP) glycoproteins. I. Studies with recombinant human ZPA, ZPB, and ZPC Laura I. Furlong,

Direct effect of macrophage migration inhibitory factor on sperm function: possible involvement in endometriosis-associated infertility Cédric Carli,

Characterization of lactoferrin receptor on human spermatozoa

Jana Peknicova, Ph. D. , Marie Pexidrova, M. Sc. , Alena Kubatova, M

Phospholipase C zeta undergoes dynamic changes in its pattern of localization in sperm during capacitation and the acrosome reaction Claire Young, B.Sc.,

Presence of the brain proteins cerebral cavernous malformation-2 and cerebral cavernous malformation-3 in rat testes and their potential role in experimental.

Changing P2X receptor localization on maturing sperm in the epididymides of mice, hamsters, rats, and humans: a preliminary study Frederick C.L. Banks,

Expression of human oocyte-specific linker histone protein and its incorporation into sperm chromatin during fertilization Yuri Mizusawa, M.D., Naoaki.

Binding of human secretory leukocyte protease inhibitor in uterine cervical mucus to immunoglobulins: pathophysiology in immunologic infertility and local.

Immunization with a DNA vaccine of testis-specific sodium-hydrogen exchanger by oral feeding or nasal instillation reduces fertility in female mice Tao.

Very low-density lipoprotein stimulates the expression of monocyte chemoattractant protein-1 in mesangial cells Edward G. Lynn, Yaw L. Siow, Dr Karmin.

Wenming Xu, Ph. D. , Xiaohu Zhang, Ph. D. , Wenying Chen, M. Sc

Nieves María Gabrielli, M. Sc. , María Florencia Veiga, Ph. D

Donna R Session, M. D. , Michael P Fautsch, Ph. D

Christophe Audouard, M.S., Elisabeth Christians, Ph.D.

Experimental immunological infertility effect of anti-GAPDH-2 antibodies on the fertility of female mice Yong Wang, Ph.D., Ning Zhang, Ph.D., Xiaodong.

Sperm content of postacrosomal WW binding protein is related to fertilization outcomes in patients undergoing assisted reproductive technology Mahmoud.

Mireille Doussau, A. I. , Andrea Lasserre, Ph. D

Recombinant human zona pellucida protein C produced in Chinese hamster ovary cells binds to human spermatozoa and inhibits sperm–zona pellucida interaction

Involvement of αvβ5 Integrin in the Establishment of Autocrine TGF-β Signaling in Dermal Fibroblasts Derived from Localized Scleroderma Yoshihide Asano,

Weon-Young Son, Ching-Tack Han, Ph. D

Mary C Mahony, Ph. D. , Dave J Swanlund, M. S. , Michelle Billeter, B

Systemic allergic reaction to coconut (Cocos nucifera) in 2 subjects with hypersensitivity to tree nut and demonstration of cross-reactivity to legumin-like.

Morphologic and functional analysis of sperm and testes in Aquaporin 7 knockout mice Eisei Sohara, M.D., Ph.D., Otoya Ueda, M.Sc., Takanori Tachibe, A.S.,

Evaluation of diagnostic testis biopsy and the repetition of testicular sperm extraction surgeries in infertility patients Alayman Hussein, M.B.B.C.H.,

Volume 56, Issue 5, Pages (November 1999)

Regulated upon activation normal T-cell expressed and secreted originating from the epididymis differentially associates with viable and defective spermatozoa

Immunologic behavior of human cysteine-rich secretory protein 1 (hCRISP1) in primates: prospects for immunocontraception Diego A. Ellerman, Ph.D., Débora.

Beer anaphylaxis Journal of Allergy and Clinical Immunology

Lama A. Youssef, B Pharm, Bridget S. Wilson, PhD, Janet M. Oliver, PhD

Characterization of D1Pas1, a mouse autosomal homologue of the human AZFa region DBY, as a nuclear protein in spermatogenic cells Donna R Session, M.D.,

Lin Mu, Ph. D. , Wei Zheng, Ph. D. , M. D. , Liang Wang, Ph. D

John Starr, MD, John H. Pruett, PhD, John W. Yunginger, MD, Gerald J

Antiacrosin antibodies and infertility. II

Insulin growth factors may be implicated in human sperm capacitation

Allergy to human seminal fluid: Cross-reactivity with dog dander

Human sperm cells express CD44

Hsien-An Pan, M. D. , Yue-Shan Lin, M. D. , Ko-Hung Lee, M. D

Characterization of D1Pas1, a mouse autosomal homologue of the human AZFa region DBY, as a nuclear protein in spermatogenic cells Donna R Session, M.D.,

Adverse reaction to lupine-fortified pasta

Daniela Zuccarello, Ph. D. , Andrea Garolla, Ph. D

Effect of incubating human sperm at room temperature on capacitation-related events Clara I. Marı́n-Briggiler, Ph.D., Jorge G. Tezón, Ph.D., Patricia.

Fertility and Sterility

Nerea Subirán, J. D. , Ekaitz Agirregoitia, J. D. , Asier Valdivia, Ph

Calcium requirements for human sperm function in vitro

William R. Boone, Ph. D. , Jeffrey M. Jones, Ph. D. , Sander S

Anti-human proacrosin antibody inhibits the zona pellucida (ZP)–induced acrosome reaction of ZP-bound spermatozoa Carolina Veaute, Ph.D., De Yi Liu,

Presentation transcript:

Fertility and Sterility Identification of human sperm proteins involved in the interaction with homologous zona pellucida Andrea Lasserre, Ph.D., Fernanda González-Echeverría, M.Sc., Carolina Moules, M.Sc., Jorge G Tezón, Ph.D., Patricia V Miranda, Ph.D., Mónica H Vazquez-Levin, Ph.D. Fertility and Sterility Volume 79, Pages 1606-1615 (June 2003) DOI: 10.1016/S0015-0282(03)00251-6

FIGURE 1 HSE proteins and immunoreactivity of anti-HSE. (A) High salt extract (HSE) components were separated in 8% SDS-PAGE and stained with CBB. Samples were reduced by adding 5% 2-β-mercaptoethanol before electrophoresis. MWM: molecular weight markers (kd). This experiment was performed at least five times obtaining similar results. A typical experiment is shown. (B) Protein extracts were subjected to 8% SDS-PAGE, followed by Western immunoblotting with anti-HSE. This experiment was Performed at least five times obtaining similar results. A typical experiment is shown. (Lane a) High salt extract (HSE) proteins. (Lane b) Proteins from human epididymis. (Lane c) Cauda epididymal plasma (CEP) proteins. (Lane d) Proteins from human testis. (Lane e) Seminal plasma proteins. Lasserre. Human sperm proteins and zona pellucida interaction. Fertil Steril 2003. Fertility and Sterility 2003 79, 1606-1615DOI: (10.1016/S0015-0282(03)00251-6)

FIGURE 2 Immunolocalization of p49 and p66 in spermatozoa. Immunocytochemical analysis of ejaculated (a, c, e) and 4-hour capacitated (b, d, f) spermatozoa stained with anti-HSE (a, b), anti-p66 (c, d), and anti-p49 (e, f) antibodies. Staining performed with preimmune serum (g: bright-field, h: immunofluorescence) (magnification, ×400). Lasserre. Human sperm proteins and zona pellucida interaction. Fertil Steril 2003. Fertility and Sterility 2003 79, 1606-1615DOI: (10.1016/S0015-0282(03)00251-6)

FIGURE 3 Immunoreactivity of anti-p49 and anti-p66 antibodies for proteins from the male reproductive tract. Protein extracts from the male reproductive tract were subjected to 8% SDS-PAGE followed by Western immunoblotting with anti-p66 (A) and anti-p49 (B) antibodies. (Lane a) High salt extract (HSE) proteins. (Lane b) Proteins from human epididymis. (Lane c) Cauda epididymal plasma (CEP) proteins. (Lane d) Proteins from human testis. (Lane e) Seminal plasma proteins. Lasserre. Human sperm proteins and zona pellucida interaction. Fertil Steril 2003. Fertility and Sterility 2003 79, 1606-1615DOI: (10.1016/S0015-0282(03)00251-6)

FIGURE 4 Immunolocalization of p66 and p49 in ejaculated and 4-hour capacitated spermatozoa. Assessment of sperm staining patterns with anti-p66 and anti-p49 antibodies was done in at least 200 spermatozoa from four semen samples. Lasserre. Human sperm proteins and zona pellucida interaction. Fertil Steril 2003. Fertility and Sterility 2003 79, 1606-1615DOI: (10.1016/S0015-0282(03)00251-6)

FIGURE 5 Effect of anti-p66 and anti-p49 antibodies on sperm–ZP interaction. Spermatozoa were preincubated with 100 μg/mL of a mixture of anti-p49 + anti-p66 antibodies or preimmune serum (control) and washed, followed by incubation with homologous ZP using the HZA. In all cases, spermatozoa tightly bound to the outer face of each HZ were counted. Results show the number of spermatozoa bound to each HZ (n = 6) (mean ± SD). Lasserre. Human sperm proteins and zona pellucida interaction. Fertil Steril 2003. Fertility and Sterility 2003 79, 1606-1615DOI: (10.1016/S0015-0282(03)00251-6)

FIGURE 6 Effect of sperm proteins p66 and p49 and HSA on sperm–ZP interaction. Spermatozoa were incubated with homologous ZP in the HZA in the presence of 10 μg/mL of sperm proteins p66 (A) or p49 (B). In all cases, spermatozoa tightly bound to the outer face of each HZ were counted. Results show the number of spermatozoa bound to each HZ (n = 5) (mean ± SD). Lasserre. Human sperm proteins and zona pellucida interaction. Fertil Steril 2003. Fertility and Sterility 2003 79, 1606-1615DOI: (10.1016/S0015-0282(03)00251-6)

FIGURE 7 Immunolocalization of p66 in human epididymis and testis. Tissue sections from distal caput (a, b, c), corpus (d, e, f) and cauda (g, h, i) epididymis, and testis (j, k, l) were immunostained with anti-p66 (a, d, g, j) and preimmune serum (b, e, h, k). Sections stained with hematoxylin (c, f, i, l) are also included (magnification, ×100). Lasserre. Human sperm proteins and zona pellucida interaction. Fertil Steril 2003. Fertility and Sterility 2003 79, 1606-1615DOI: (10.1016/S0015-0282(03)00251-6)