Knockdown efficiency of different shRNAs.

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Knockdown efficiency of different shRNAs. Knockdown efficiency of different shRNAs. (A–C) qRT-PCR showing the mRNA expression of CANP1 (A), CAPN7 (B), and CTSL (C) in HEK293 cells after transfection of shRNA targeting CAPN1, CAPN7, and CTSL, respectively. Two shRNAs were used for each gene, which all resulted in significant knockdown of the gene expression. Data were presented as mean ± SEM, n = 3. **P < 0.01, ***P < 0.001, as compared with the shRNA-Con group by two-tailed t test. Yiran Wang et al. LSA 2019;2:e201800268 © 2019 Wang et al.