Glucose uptake and expression of thermogenesis markers are increased in the BAT of HFD-fed NprcAKO mice. Glucose uptake and expression of thermogenesis.

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Glucose uptake and expression of thermogenesis markers are increased in the BAT of HFD-fed NprcAKO mice. Glucose uptake and expression of thermogenesis markers are increased in the BAT of HFD-fed NprcAKO mice. (A) Glucose infusion rate of NprcAKO (n = 10) and Nprcfl/fl (n = 8) mice during a hyperinsulinemic-euglycemic clamp experiment performed at 12 weeks of HFD feeding. (B and C) Rates of glucose disposal (Rd) (B) and endogenous glucose appearance (EndoRa) (C) in NprcAKO (n = 10) and Nprcfl/fl (n = 8) mice under basal and clamp states. (D) Rate of glucose uptake (Rg) in the BAT, iWAT, gWAT, gastrocnemius (GA), superficial vastus lateralis (SVL), soleus (SO), heart, and brain of NprcAKO (n = 10) and Nprcfl/fl (n = 8) mice at the end of the clamp experiment. (E) Western blot analysis of thermogenic and mitochondrial proteins in the BAT of NprcAKO and Nprcfl/fl mice after 12 weeks on HFD. Blots are representative of three independent experiments. COX4, cytochrome c oxidase subunit 4; CYTOC, cytochrome c; NDUFS4, NADH (reduced form of nicotinamide adenine dinucleotide) dehydrogenase ubiquinone iron-sulfur protein 4; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. (F) Immunostaining and quantification of UCP1 in the BAT of NprcAKO (n = 2) and Nprcfl/fl (n = 2) mice after 12 weeks on HFD, as described in Materials and Methods. Scale bar, 200 μm. (G) qRT-PCR for the expression of genes coding thermogenic, mitochondrial, fatty acid oxidation (FAOx) markers, and BAT-derived adipokines in the BAT of NprcAKO (n = 14) and Nprcfl/fl (n = 7) mice after 12 weeks on HFD. Data were relative to 36B4. *P < 0.05; **P < 0.01; ***P < 0.001. P values of glucose infusion rate were obtained by one-tailed Student’s t test. P values of Rd, Ra, Rg, and relative mRNA expression were calculated using unpaired two-tailed Student’s t tests. Wei Wu et al., Sci. Signal. 2017;10:eaam6870 ©2017 by American Association for the Advancement of Science