Differential regulation of cytokine expression after allergen exposure of sensitized rats by cyclosporin A and corticosteroids: Relationship to bronchial.

Slides:

Advertisements

Similar presentations

Dual effects of vitamin D–induced alteration of TH1/TH2 cytokine expression Victor Matheu, MD, Ove Bäck, MD, PhD, Emma Mondoc, BSc, Shohreh Issazadeh-Navikas,

Advertisements

Sejal Saglani, MD, Stephen Lui, PhD, Nicola Ullmann, MD, Gaynor A

Regulation of TH2 responses by the pulmonary Clara cell secretory 10-kd protein Chih-Hsing Hung, MD, Li-Chen Chen, MD, Zhongjian Zhang, PhD, Bhabadeb.

Different expression of cytokine and membrane molecules by circulating lymphocytes on acute mental stress in patients with atopic dermatitis in comparison.

Doxycycline reduces airway inflammation and hyperresponsiveness in a murine model of toluene diisocyanate–induced asthma Kyung S. Lee, MS, Sun M. Jin,

Diesel exhaust particulate induces airway hyperresponsiveness in a murine model: Essential role of GM-CSF Ken Ohta, MD, PhD, Naomi Yamashita, MD, PhD,

The effects of upper respiratory infection on T-cell proliferation and steroid sensitivity of asthmatics Elcio O. Vianna, MD, PhD, Jay Westcott, PhD,

Christine M. Braun, BA, Shau-Ku Huang, PhD, Gregory G

Local administration of antisense phosphorothioate oligonucleotides to the c-kit ligand, stem cell factor, suppresses airway inflammation and IL-4 production.

Effect of interleukin-5 and granulocyte-macrophage colony stimulating factor on in vitro eosinophil function: Comparison with airway eosinophils Julie.

Expression of IL-4 receptor α on smooth muscle cells is not necessary for development of experimental allergic asthma Frank Kirstein, PhD, William G.C.

Frank Kirstein, PhD, Natalie E

Nazanin Farhadi, MSc, Laura Lambert, BA, Chiara Triulzi, PhD, Peter J

IL-33 dysregulates regulatory T cells and impairs established immunologic tolerance in the lungs Chien-Chang Chen, PhD, Takao Kobayashi, PhD, Koji Iijima,

Restoration of T-box–containing protein expressed in T cells protects against allergen- induced asthma Jung Won Park, MD, Hyun Jung Min, MS, Jung Ho Sohn,

Antigen-specific effector CD8 T cells regulate allergic responses via IFN-γ and dendritic cell function Yafang Tang, BSc, Shou Ping Guan, BSc, Benson.

Β-Glucan exacerbates allergic asthma independent of fungal sensitization and promotes steroid-resistant TH2/TH17 responses Zhonghua Zhang, MD, Jocelyn.

Responsiveness to respiratory syncytial virus in neonates is mediated through thymic stromal lymphopoietin and OX40 ligand Junyan Han, PhD, Azzeddine.

Grass pollen immunotherapy inhibits allergen-induced infiltration of CD4+ T lymphocytes and eosinophils in the nasal mucosa and increases the number of.

Dendritic cells and alveolar macrophages mediate IL-13–induced airway inflammation and chemokine production Margaret Crapster-Pregont, BS, Janice Yeo,

Surfactant protein D inhibits TNF-α production by macrophages and dendritic cells in mice László Hortobágyi, MS, Sonja Kierstein, PhD, Kateryna Krytska,

Histamine in the immune regulation of allergic inflammation

Prime role of IL-17A in neutrophilia and airway smooth muscle contraction in a house dust mite–induced allergic asthma model Julie Chesné, PhD, Faouzi.

Frank Kirstein, PhD, Natalie E

Requirements for allergen-induced airway inflammation and hyperreactivity in CD4- deficient and CD4-sufficient HLA-DQ transgenic mice Svetlana P. Chapoval,

Cristiana Stellato, MD, PhDa, Jun Atsuta, MD, PhDb, Carol A

Cellular and mediator responses twenty-four hours after local endobronchial allergen challenge of asthmatic airways Anthony J. Frew, MD, Johanne St-Pierre,

MicroRNA-9 regulates steroid-resistant airway hyperresponsiveness by reducing protein phosphatase 2A activity Jing Jing Li, MS, Hock L. Tay, PhD, Steven.

Takao Kobayashi, PhD, Koji Iijima, PhD, Alexander L

Corticosteroid-resistant asthma is associated with classical antimicrobial activation of airway macrophages Elena Goleva, PhD, Pia J. Hauk, MD, Clifton.

Exaggerated IL-17 response to epicutaneous sensitization mediates airway inflammation in the absence of IL-4 and IL-13 Rui He, MD, PhD, Hye Young Kim,

Janus kinase 1/3 signaling pathways are key initiators of TH2 differentiation and lung allergic responses Shigeru Ashino, PhD, Katsuyuki Takeda, MD,

Involvement of ion channels in human eosinophil respiratory burst

IL-11 expression is increased in severe asthma: Association with epithelial cells and eosinophils Eleanor Minshall, PhDa, Jamila Chakir, PhDb, Michel.

Mukesh Kumar, PhDa, Aruna K. Behera, PhDa, Jianan Hu, MDa, Richard F

Cell surface characterization of T lymphocytes and allergen-specific T cell clones: Correlation of CD26 expression with T H1 subsets Martin Willheim,

Latent adenoviral infection modifies the steroid response in allergic lung inflammation Katsuhiko Yamada, MDa, W.Mark Elliott, PhDa, Shizu Hayashi, PhDa,

IL-35 production by inducible costimulator (ICOS)–positive regulatory T cells reverses established IL-17–dependent allergic airways disease Gregory S.

Corticosteroid-induced apoptosis in mouse airway epithelium: Effect in normal airways and after allergen-induced airway inflammation Delbert R. Dorscheid,

Genetic susceptibility to food allergy is linked to differential TH2-TH1 responses in C3H/HeJ and BALB/c mice Vivian Morafo, PhD*, Kamal Srivastava,

Mast cells regulate IFN-γ expression in the skin and circulating IgE levels in allergen- induced skin inflammation Harri Alenius, PhD, Dhafer Laouini,

Effect of rush immunotherapy on airway inflammation and airway hyperresponsiveness after bronchoprovocation with allergen in asthma Yasurou Kohno, MDa,

Adoptively transferred late allergic response is inhibited by IL-4, but not IL-5, antisense oligonucleotide Sophie Molet, PhD, David Ramos-Barbón, MD,

IL-10–treated dendritic cells decrease airway hyperresponsiveness and airway inflammation in mice Toshiyuki Koya, MD, PhD, Hiroyuki Matsuda, MD, PhD,

TH1/TH2 cytokines and inflammatory cells in skin biopsy specimens from patients with chronic idiopathic urticaria: Comparison with the allergen-induced.

Antibody to very late activation antigen 4 prevents interleukin-5–induced airway hyperresponsiveness and eosinophil infiltration in the airways of guinea.

Fractional analysis of sequential induced sputum samples during sputum induction: Evidence that different lung compartments are sampled at different time.

Potential role of interleukin-1 in allergen-induced late asthmatic reactions in guinea pigs: Suppressive effect of interleukin-1 receptor antagonist on.

Inhibition of human allergic T-cell responses by IL-10–treated dendritic cells: Differences from hydrocortisone-treated dendritic cells Iris Bellinghausen,

Ajay B. Antony, HSa, Robert S. Tepper, MDb, Kamal A. Mohammed, PhDa

Duy Pham, PhD, Sarita Sehra, PhD, Xin Sun, PhD, Mark H. Kaplan, PhD

Effects of diesel exhaust on allergic airway inflammation in mice

Comparison of effects of topical levocabastine and nedocromil sodium on the early response in a conjunctival provocation test with allergen Catherine.

13-Hydroxy-linoleic acid induces airway hyperresponsiveness to histamine and methacholine in guinea pigs in vivo Paul A.J. Henricks, PhDa, Ferdi Engels,

Inhibition of allergic airways inflammation and airway hyperresponsiveness in mice by dexamethasone: Role of eosinophils, IL-5, eotaxin, and IL-13 Seok-Yong.

Changes in sputum counts and airway hyperresponsiveness after budesonide: Monitoring anti-inflammatory response on the basis of surrogate markers of airway.

Suplatast tosilate inhibits goblet-cell metaplasia of airway epithelium in sensitized mice Jae Jeong Shim, MD, Karim Dabbagh, PhD, Kiyoshi Takeyama, MD,

Nedocromil sodium and airway inflammation in vivo and in vitro

Monocyte chemotactic proteins in allergen-induced inflammation in the nasal mucosa: Effect of topical corticosteroids Pota Christodoulopoulos, BSca,

Zhenying Nie, PhD, Cole S. Nelson, PhD, David B. Jacoby, MD, Allison D

Matrix metalloproteinase inhibitor regulates inflammatory cell migration by reducing ICAM-1 and VCAM-1 expression in a murine model of toluene diisocyanate-induced.

Abnormalities of cell and mediator levels in bronchoalveolar lavage fluid of patients with mild asthma Sally E. Wenzel, MD Journal of Allergy and Clinical.

Effect of continuing or finishing high-level sports on airway inflammation, bronchial hyperresponsiveness, and asthma: A 5-year prospective follow-up.

Dexamethasone suppresses gene expression and production of IL-13 by human mast cell line and lung mast cells Toshiaki Fushimi, MD, Hiroshi Okayama, MD,

IL-4– and IL-5–positive T lymphocytes, eosinophils, and mast cells in allergen-induced late-phase cutaneous reactions in atopic subjects Luis T. Barata,

Salmeterol attenuates chemotactic responses in rhinovirus-induced exacerbation of allergic airways disease by modulating protein phosphatase 2A Luke.

Reciprocal regulation of cultured human mast cell cytokine production by IL-4 and IFN-γ Hiroshi Tachimoto, MD, PhDa, Motohiro Ebisawa, MD, PhDa,b, Tomohide.

Mechanism of topical glucocorticoid treatment of hay fever: IL-5 and eosinophil activation during natural allergen exposure are suppressed, but IL-4,

Annette M. Colavita, MD, Annette T. Hastie, PhD, Ali I

Local treatment with IL-12 is an effective inhibitor of airway hyperresponsiveness and lung eosinophilia after airway challenge in sensitized mice Jürgen.

Presentation transcript:

Differential regulation of cytokine expression after allergen exposure of sensitized rats by cyclosporin A and corticosteroids: Relationship to bronchial hyperresponsiveness Tung-Jung Huang, MDab, Robert Newton, PhDa, El-Bdaoui Haddad, PhDa, K.Fan Chung, MDa Journal of Allergy and Clinical Immunology Volume 104, Issue 3, Pages 644-652 (September 1999) DOI: 10.1016/S0091-6749(99)70337-4 Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 1 Bronchial responsiveness in CsA study (A) and dexamethasone study (B). Left, Mean percent increase in RL to increasing concentrations of ACh for different groups of rats. A, CsA study: NO, nonsensitized and OA exposed, n = 9, open circles; SS, OA sensitized and sodium chloride solution exposed, n = 7, open squares; SOO, sensitized, olive oil treated, and 1% OA exposed, n = 9, solid squares; and SOC, sensitized, CsA treated and OA exposed, n = 10, solid diamonds. B, Dexamethasone study: SS, OA sensitized and sodium chloride solution exposed, n = 7, open squares; SO, sensitized, sodium chloride solution treated, and 1% OA exposed, n = 7, solid squares; and SOD, sensitized, dexamethasone treated, and OA exposed, n = 7, open diamonds. Concentration-response curves are significantly shifted leftward for groups SOO, SOC, and SO. Right, Mean –logPC200 , which is negative logarithm of provocative concentration of ACh needed to increase baseline lung resistance by 200%, for groups of rats as detailed above. Dexamethasone, but not CsA, inhibited allergen-induced increase in –logPC200 . Asterisk, P < .05 as groups SOO and SOC compared with SS in A or SO compared with SS and SOD in B; two asterisks, P < .001 as SOO compared with NO and SS. Data shown as mean ± SEM. Journal of Allergy and Clinical Immunology 1999 104, 644-652DOI: (10.1016/S0091-6749(99)70337-4) Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 2 Mean cell numbers of total cell, macrophage (Mac), eosinophil (Eos), lymphocyte (Lym), and neutrophil (Neu) in BAL fluid in CsA study (A) and dexamethasone study (B) for groups of rats as specified in Fig 1. CsA treatment suppressed increase in eosinophil and lymphocyte counts induced by allergen exposure of sensitized rats in BAL fluid but had no effect on neutrophil influx. Dexamethasone also suppressed induced increases in eosinophil, lymphocyte, and neutrophil influx. A, Asterisk, P < .005 compared with NO and SS groups; two asterisks, P < .001 compared with SOC group; pound sign, P < .04 compared with NO group. B, Asterisk, P < .05 compared with other groups; two asterisks, P < .02 compared with groups SS and SOD; pound sign, P < .04 compared with group SS. Data shown as mean ± SEM. Journal of Allergy and Clinical Immunology 1999 104, 644-652DOI: (10.1016/S0091-6749(99)70337-4) Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 3 Mean eosinophil (left) and T-lymphocyte subsets (CD2+, CD4+, and CD8+, right ) counts in airway submucosa expressed as square millimeter in CsA study (A) and dexamethasone study (B) with groups as detailed in Fig 1. There was significant increase in eosinophils in airway submucosa after allergen challenge of sensitized rats, which was suppressed by pretreatment with CsA and dexamethasone. Allergen challenge led to significant increase in total number of T lymphocytes expressing pan T-cell marker CD2+ and also CD8+ T-lymphocytes. Although CsA did not significantly reduce CD2+ T cells, there was significant inhibition of increase in CD4+ T cells and CD8+ T cells induced by allergen exposure. In contrast, dexamethasone significantly inhibited increase in all 3 subsets of T lymphocytes. Asterisk, P < .05 compared with other groups; two asterisks, P < .03 compared with group SOO or SO; pound sign, P < .03 compared with NO and SS groups in CsA study. Data shown as mean ± SEM. Journal of Allergy and Clinical Immunology 1999 104, 644-652DOI: (10.1016/S0091-6749(99)70337-4) Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 4 Mean IL-2, IL-4, IL-5, IL-10, and IFN-γ mRNA expression in rat lung expressed as ratio to GAPDH mRNA, as determined by RT-PCR, followed by Southern blot analysis. Expression was obtained as radioactivity count of dot-blot analysis of PCR products. Representative bands are shown on left (2 rats from each group), and mean mRNA expression with SEM is shown on right. Groups of rats were as for Fig 1. There was significant increase in expression of IL-2, IL-4, IL-5, and IFN-γ after allergen exposure of sensitized rats (groups SOO and SO), an effect that was inhibited by CsA. Dexamethasone suppressed expression of IL-2, IL-4, and IL-5 mRNA but increased IL-10 expression, together with nonsignificant further increase in IFN-γ mRNA expression. Asterisk, P < .05 compared with other groups. Journal of Allergy and Clinical Immunology 1999 104, 644-652DOI: (10.1016/S0091-6749(99)70337-4) Copyright © 1999 Mosby, Inc. Terms and Conditions