Efficient CRISPR mutagenesis in C. glabrata.

Slides:

Advertisements

Similar presentations

Shigella-specific serum IgG and IgA endpoint titers in guinea pigs immunized intranasally with S. flexneri 2a InvaplexNAT or InvaplexAR. Shigella-specific.

Advertisements

2 point perspective.

PCR genotype analysis to determine RNP-mediated knockout efficiency in C. lusitaniae. PCR genotype analysis to determine RNP-mediated knockout efficiency.

High efficiency of gene deletion in all tested genetic backgrounds of A. fumigatus. High efficiency of gene deletion in all tested genetic backgrounds.

CRISPR/Cas targeting of RFP in C. albicans.

MFS1 expression in IPO323 MFS1 replacement mutants.

Design and use of a high-throughput screen to identify tumor cell-induced gene activation events in Salmonella. Design and use of a high-throughput screen.

(A to G) TEM on negatively stained whole spores of C

lgc-35 (L324S) CRISPR-Cas9-RNP genotyping and Mendelian segregation.

DNSP16 mutation attenuates the virulence of a mouse-adapted MERS-CoV strain. dNSP16 mutation attenuates the virulence of a mouse-adapted MERS-CoV strain.

Antimicrobial zone of inhibition.

Spores of C. difficile imaged by TEM and SEM

TEM on spore section of strain ATCC 9714.

Phylogeny of Shiga toxin-encoding phage from SDi/SJo S. sonnei isolates. Phylogeny of Shiga toxin-encoding phage from SDi/SJo S. sonnei isolates. (A) progressiveMauve.

SEM images of S. aureus ATCC cells left untreated (A) or treated with polymyxin B (PMB; 99.5 mg/liter) (B), FADDI-019 (1× MIC) (C), FADDI-019 (4×

Vectors for CRISPR mutagenesis in Candida albicans, Candida glabrata, Naumovozyma castellii, and Saccharomyces cerevisiae. Vectors for CRISPR mutagenesis.

Synthetic perturbation of DE and DV genes.

Gap2 is required for growth on phenylalanine as the sole source of nitrogen. Gap2 is required for growth on phenylalanine as the sole source of nitrogen.

Unified Solo vectors for mutagenesis in C. albicans.

Spores of strain ATCC 9714 imaged by SEM (A), cryo-EM (B), and 3D-SIM (C and D) with spores labeled with lipid dyes DiO and FM 4-64FX (C) or DiO and spore-specific.

Growth of L. monocytogenes strains AL4E, AT3E, and 10403S, derivative cured strain AT3Epc, and conjugation strains 10403SpclpL and 10403SpPL2 displayed.

RAD51 is essential for L. donovani.

The virulence of Candida species in Galleria mellonella larvae at 37°C is species specific. The virulence of Candida species in Galleria mellonella larvae.

Schematic depiction of the effect of different types of Duchenne muscular dystrophy (DMD)-causing mutations on the dystrophin transcript. Schematic depiction.

Cell wall hydrolytic activities of Andhra lysins.

RFP CRISPR mutagenesis as a function of sgRNA delivery.

Comparative analysis of Aeromonas sp.

Mutagenesis through Cas9/sgRNA-induced HDR

Influence of PDR1 hyperactivity and EPA1 on the colonization of the bladder and kidney by C. glabrata in the murine urinary tract infection (UTI) model.

Side-view and apical-view projections of mutant and validation strain biofilms. Side-view and apical-view projections of mutant and validation strain biofilms.

Tagging of the endogenous Py03652 gene with the gfp gene in Plasmodium yoelii. Tagging of the endogenous Py03652 gene with the gfp gene in Plasmodium yoelii.

Southern blot analysis of ΔpksP mutant generated in the Af293 background. Southern blot analysis of ΔpksP mutant generated in the Af293 background. (A)

Stress-protective roles of C. auris Hog1.

DuMPLING oligonucleotide plasmid library design and production

Deletion of the multicopy A2 family genes and increased efficiency through coselection for parasites with CRISPR-Cas9 activity. Deletion of the multicopy.

Generation of heterozygous mutations with the CRISPR-Cas9 system.

A case study of RPCA feature loadings on real data sets; sponge (left; A and C) and sleep apnea (right; B and D). A case study of RPCA feature loadings.

Phenotypic consequences of the most common rpoB mutants.

Average forces for fins with fin rays of different stiffnesses at a flow rate of 90 mm s–1 and beat frequencies of 0.50, 0.65, 1.00, 1.30, and 1.60 Hz.

Growth of Salmonella and E. coli in egg white.

The ribosomal RNA promoter is more efficient than the U6 promoter to drive gRNA expression in Leishmania. The ribosomal RNA promoter is more efficient.

Superspacers shared between viral strains.

Time course analysis of the response in V1 to the M and P stimuli for a single subject. Time course analysis of the response in V1 to the M and P stimuli.

Editing and reconstitution of CPAR2_

Editing of ADE2 in C. metapsilosis.

Susceptibility to hydrogen peroxide.

SusG LES is required for efficient packing into OMVs

SAM transport by Gap4 in S. cerevisiae cells.

Average forces for fins with fin rays of different stiffness at a fin-beat frequency of 1.00 Hz, and flow rates of 0, 90, 180, and 270 mm s–1. Average.

Low pH alters Ras1 localization and its activation state, but these changes are not responsible for the effect of pH on morphology. Low pH alters Ras1.

Stress resistance comparisons.

KEGG cluster of DEGs of S

Sequence comparisons of fungal HOG1 orthologues.

The pCT-tRNA plasmid system for gene editing in C. tropicalis.

MMEJ plays a dominant role in double-strand DNA break repair in L

dCas9-mediated repression of transcription.

Mammalian cell cytotoxicity shown by macrophage response to DM262.

LEfSe comparison analysis between the control and ciprofloxacin or vancomycin-imipenem groups at the end of antibiotic treatment (A or B, respectively)

Interrogation of F. philomiragia and H

Genotype analysis of transient CRISPR system transformants.

Transient CRISPR-Cas9 system.

Distribution of selected traits across the >4,000 strains in the most recent version of the database, including cell shape (A), spore formation (B), motility.

Production of ade2Δ/ade2Δ mutants by the Candida CRISPR system.

Prediction of cross-resistance among rifampin-resistant isolates.

Mixed head-to-head competitions.

Proteus mirabilis can utilize d-serine as a sole source of carbon and nitrogen. Proteus mirabilis can utilize d-serine as a sole source of carbon and nitrogen.

Effect on the recall response of Nrp1 deletion before infection.

Knock-in of the rpl42-P56Q mutation using the split-ura4 system.

Confirmation of spliced RNA in cells transfected with a wild-type or Pol(−) MR766 ZIKV plasmid. Confirmation of spliced RNA in cells transfected with a.

Presentation transcript:

Efficient CRISPR mutagenesis in C. glabrata. Efficient CRISPR mutagenesis in C. glabrata. Strain BG2 was transformed with pV1326 (top left), pV1329 (top middle), pV1329 with a stop-codon repair template (top right), pV1435 (bottom middle), or pV1435 with a stop-codon repair template (bottom right). The yellow box (bottom left) presents a magnified view of a pV1329 with stop-codon repair transformation plate (top right). Similar results were seen with strain CLIB138. Vectors depict CaCas9 promoter differences between the two plasmids. Valmik K. Vyas et al. mSphere 2018; doi:10.1128/mSphere.00154-18