Production of Mapk14 and Mapk7 KO cells.

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Production of Mapk14 and Mapk7 KO cells. Production of Mapk14 and Mapk7 KO cells. (A)Mapk14 conditional KO mice used in the experiment. Exon 2 of the Mapk14 gene was deleted by CRE-mediated recombination (moi = 2). The indicated primers were used for PCR analysis. (B) Deletion efficiency of the target gene at the time of transplantation by real-time PCR of genomic DNA (n = 4 testes). Exon 12 was used as a control. *P < 0.05 (t test). (C)Mapk7 conditional KO mice used in the experiment. Exon 4 of the Mapk7 gene was deleted by CRE-mediated recombination (moi = 2). (D) Deletion efficiency of the target gene at the time of transplantation by real-time PCR of genomic DNA (n = 12 cultures). Exon 1 was used as a control. *P < 0.05 (t test). Hiroko Morimoto et al. LSA 2019;2:e201900374 © 2019 Morimoto et al.