Supraphysiological levels of GDF11 cause atrophy of striated muscle in vivo Supraphysiological levels of GDF11 cause atrophy of striated muscle in vivo.

Slides:

Advertisements

Similar presentations

Hyp mice show hypertension and increased NCC expression and phosphorylation A–ESerum intact Fgf23 concentration (A, n = 8–9, Student's t‐test, *P =

Advertisements

Effects of inhibiting inflammatory cell recruitment on fracture healing Treatment with CCR2 antagonist, INCB3344, led to impaired fracture healing compared.

Impact of triple combination of gemcitabine‐based chemoirradiation with anti‐PD‐L1 in the KPC tumor allograft Impact of triple combination of gemcitabine‐based.

Morphology of white adipose tissue and muscle in S1/3 KO mice of different ages Morphology of white adipose tissue and muscle in S1/3 KO mice of different.

Loss of Hdac3 impairs Ar signaling in mouse prostate tissues and has no effect on apoptosis Loss of Hdac3 impairs Ar signaling in mouse prostate tissues.

Metabolic effects of TUG‐891 are reduced or absent in GPR120‐deficient mice Metabolic effects of TUG‐891 are reduced or absent in GPR120‐deficient mice.

Volume 18, Issue 3, Pages (September 2013)

Volume 11, Issue 8, Pages (May 2015)

PERK activator decreases pathological tau species in vivo

PbA‐specific CD4 T‐cell responses are maximal at day 6 post‐pRBC infection PbA‐specific CD4 T‐cell responses are maximal at day 6 post‐pRBC infection Proportion.

Treatment with p38γ/p38δ inhibitor shows antifungal effects in vivo

[68Ga]Pentixafor PET imaging of MM xenografts Real‐time PCR analysis of cxcr4 transcript expression levels in HeLa (negative control) and in MM cell lines.

Reverse cholesterol transport is enhanced after systemic miR‐33 silencing.C57BL/6 mice (n = 7) were infused i.v. with 5 mpk scrambled or anti‐miR‐33 oligonucleotides.

TFE3 regulates glucose homeostasis

Expression levels of the transgenic DIA1(R1204X) mutant in the inner ear of TG mice Expression levels of the transgenic DIA1(R1204X) mutant in the inner.

AAV8‐hAAT‐FGF21 treatment improves liver fibrosis

Supplementary Figure I

Differential accumulation of substrates in vivo

Metabolic profile of Tfe3 KO mice fed a HFD

Metabolic profile of Tfe3 KO mice

Phenogenetic networks of genes linked to Alzheimer's and Parkinson's disease reveal concordant phenotypes Phenogenetic networks of genes linked to Alzheimer's.

Clinical testing of calcineurin inhibitors cyclosporine A and tacrolimus in autoimmune disorders Clinical testing of calcineurin inhibitors cyclosporine.

TFE3 prevents diet‐induced obesity and metabolic syndrome

Impaired glucose tolerance in adult S1/3 KO mice on a normal chow

Negligible T22‐GFP‐H6‐FdU biodistribution or toxicity on non‐tumor tissues Negligible T22‐GFP‐H6‐FdU biodistribution or toxicity on non‐tumor tissues AUndetectable.

KLK7 is involved in the Aβ degradation activity

Expression of PD‐1 on PBMC and malignant exudate T cells

SEMA3C silencing inhibits CRPC cell growth and induces apoptosis

Combination of OTX‐015 and PD does not induce overt toxicity

PERK activator treatment protects neurite network

Dietary administration of DHEA reduces jet‐lag

Molecular and clinical characterization of AAV2/8‐TBG‐h

Molecular and morphological analysis of Pitrm1+/− mouse brain

Rescuing mGPDH deficiency improves skeletal muscle regeneration during obesity and diabetes Rescuing mGPDH deficiency improves skeletal muscle regeneration.

Diversity of methods utilized to model neurological diseases using patient‐derived iPSCs Diversity of methods utilized to model neurological diseases using.

Impaired ECM protein synthesis in infarcted area of S100a4cre+/− × Fstl1flox/flox mice Impaired ECM protein synthesis in infarcted area of S100a4cre+/−

Treatment with AAV8‐hAAT‐FGF21 improves insulin sensitivity and glucose tolerance Treatment with AAV8‐hAAT‐FGF21 improves insulin sensitivity and glucose.

Effects of in vivo Nutlin‐3 treatment on CSC content of Numb− and Numb+ tumors Effects of in vivo Nutlin‐3 treatment on CSC content of Numb− and Numb+

Volume 82, Issue 4, Pages (August 2012)

TGF‐β1 treatment induces the chromatin binding of Smad2/3/4 in 4T1 cells TGF‐β1 treatment induces the chromatin binding of Smad2/3/4 in 4T1 cells A, BWestern.

The preventive role of TR in HFD‐treated mice

IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice ARepresentative.

Potential relevance of SV‐eQTLs in adaptive or maladaptive pathways in heart failure Potential relevance of SV‐eQTLs in adaptive or maladaptive pathways.

LV.IDS and LV.IDS.ApoEII improve brain‐specific IDS activity and express supra‐physiological levels of active IDS in peripheral organs LV.IDS and LV.IDS.ApoEII.

mGPDH is not essential to muscle development

PGC‐1α‐independent regulation of the slow‐twitch muscle fiber program by miR‐499 PGC‐1α‐independent regulation of the slow‐twitch muscle fiber program.

Volume 18, Issue 1, Pages (January 2010)

GPR120 deficiency alters the expression of genes involved in glucose and lipid metabolism in BAT GPR120 deficiency alters the expression of genes involved.

C266S laforin's effect on LD‐associated general defect in autophagy

I.c.v.‐injected AβOs induce increased food intake, hypothalamic expression of orexigenic neuropeptides but no hypothalamic cell degeneration AAccumulated.

Therapeutic role of TR in HFD‐established diabetic mice

Rescue of motor neurons from death The ventral root of the fifth lumbar segment (L5). Rescue of motor neurons from death The ventral root of the fifth.

Treatment with AZD5363 reduces the proportion of proliferating vascular endothelial cells and following RT, the influx of CD11b+ bone marrow‐derived cells.

Tissue‐specific differences in the levels of CoQ9, CoQ10, and DMQ9, and in the DMQ9/CoQ9 ratio after β‐RA treatment in Coq9R239X mice Tissue‐specific differences.

FGFR1 and TGFβ signaling activity in smooth muscle cells in a mouse atherosclerosis model FGFR1 and TGFβ signaling activity in smooth muscle cells in a.

GDF11 induces myotube atrophy in vitro

CNP520 treatment of male C57/BL6 mice for 8 weeks did not cause hair depigmentation CNP520 treatment of male C57/BL6 mice for 8 weeks did not cause hair.

CAR T‐cell generation in human PBMC‐transplanted mice

Mice with AS160/TBC1D4-Thr649Ala Knockin Mutation Are Glucose Intolerant with Reduced Insulin Sensitivity and Altered GLUT4 Trafficking Shuai Chen, David.

Limiting neutrophilic inflammation during active phase (ZT13) reduces MI damage Limiting neutrophilic inflammation during active phase (ZT13) reduces MI.

KlbHET mice exhibit altered estrous cycle and subfertility

SEMA3C regulates prostate cancer cell growth

Disturbance of mTOR signaling in vivo in GAA‐KO mice

Immobilization decreases the global rates of protein synthesis and muscle mass, but activates mTOR signaling and cap-dependent translation. Immobilization.

Impaired Mitochondrial Fat Oxidation Induces FGF21 in Muscle

Volume 23, Issue 8, Pages (August 2015)

Quan Jin, Chunping Qiao, Jianbin Li, Juan Li, Xiao Xiao

AKT thr308 phosphorylation related to total AKT2 protein in SOL muscle (m. soleus) (A) and EDL muscle (m. extensor digitorum longus) (D) and AKT ser472.

Fig. 1. GDF15 is up-regulated with obesity, and AAV-GDF15 improves metabolic parameters in DIO mice. GDF15 is up-regulated with obesity, and AAV-GDF15.

Axial T1-weighted MRI of the thigh (A) and lower leg (B) for case 1 shows fatty infiltration of the quadriceps and adductor muscles (L2, 3 and 4 myotomes;

Presentation transcript:

Supraphysiological levels of GDF11 cause atrophy of striated muscle in vivo Supraphysiological levels of GDF11 cause atrophy of striated muscle in vivo ATwelve‐week‐old male C57BL/6 mice (n = 3) were injected i.p. with PBS (control) or 1 × 1012 gc of a liver‐specific GDF11 packaged in AAV2/8 (AAV8.GDF11), and were euthanized seven days after treatment.B–DImmunoblotting of IgG‐reduced samples (see Materials and Methods) reveals an increase in GDF11 content in AAV8.GDF11‐treated liver (B), serum (C), and quadriceps (D), as detected by the R&D Systems GDF11 mouse mAb under reducing (50 mM DTT) conditions. The identifiable bands of full‐length GDF11 and monomeric GDF11 in the immunoblots are labeled, while an ambiguous 25 kDa band is marked with a star (*). GAPDH immunoblotting is shown to demonstrate equal loading among lanes.E, FSeven days after, AAV8.GDF11 treatment resulted in substantial losses in body weight (E), and muscle mass of the soleus (Sol), extensor digitorum longus (EDL), tibialis anterior (TA), gastrocnemius (Gas), quadriceps (Quad), and heart (F). Values depicted are mean ± SEM. Statistical analysis was performed using two‐tailed Student's t‐test with effect size presented as Cohen's d (d). David W Hammers et al. EMBO Mol Med. 2017;emmm.201607231 © as stated in the article, figure or figure legend